Analysis of blood-induced anopheles gambiae midgut proteins and sexual stage plasmodium falciparum interaction reveals mosquito genes important for malaria transmission

ABSTRACT _Plasmodium_ invasion of mosquito midguts is a mandatory step for malaria transmission. The roles of mosquito midgut proteins and parasite interaction during malaria transmission

are not clear. This study aims to identify mosquito midgut proteins that interact with and affect _P. falciparum_ invasion. Based on gene expression profiles and protein sequences, 76

mosquito secretory proteins that are highly expressed in midguts and up-regulated by blood meals were chosen for analysis. About 61 candidate genes were successfully cloned from _Anopheles

gambiae_ and expressed in insect cells. ELISA analysis showed that 25 of the insect cell-expressed recombinant mosquito proteins interacted with the _P. falciparum_-infected cell lysates.

Indirect immunofluorescence assays confirmed 17 of them interacted with sexual stage parasites significantly stronger than asexual stage parasites. Knockdown assays found that seven

candidate genes significantly changed mosquitoes' susceptibility to _P. falciparum_. Four of them (AGAP006268, AGAP002848, AGAP006972, and AGAP002851) played a protective function

against parasite invasion, and the other three (AGAP008138, FREP1, and HPX15) facilitated _P. falciparum_ transmission to mosquitoes. Notably, AGAP008138 is a unique gene that only exists in

_Anopheline_ mosquitoes. These gene products are ideal targets to block malaria transmission. SIMILAR CONTENT BEING VIEWED BY OTHERS IDENTIFICATION AND PRELIMINARY CHARACTERIZATION OF

_PLASMODIUM FALCIPARUM_ PROTEINS SECRETED UPON GAMETE FORMATION Article Open access 10 June 2022 MOLECULAR INTERACTIONS BETWEEN PARASITE AND MOSQUITO DURING MIDGUT INVASION AS TARGETS TO

BLOCK MALARIA TRANSMISSION Article Open access 29 November 2021 A SINGLE-CELL ATLAS OF _PLASMODIUM FALCIPARUM_ TRANSMISSION THROUGH THE MOSQUITO Article Open access 27 May 2021 INTRODUCTION

Malaria causes approximately half a million deaths globally each year. Most of the deaths occur in sub-Saharan Africa, and around 95% of deaths are children under the age of five. Although

the death rate has dropped by 54% between 2000 and 2013 due to anti-malaria drugs, insecticide-treated nets, and indoor insecticide spraying1, malaria control and elimination still face

several challenges. At present, vector control strategies are not as effective as it was because of the spread of insecticide-resistant mosquitoes2. The control of malaria also faces the

challenge of the resistant _Plasmodium falciparum_ strains. Therefore, the malaria eradication campaign needs new strategies. Transmission intervention by vaccines, compounds, or transgenic

mosquitoes, is a promising approach. However, valid molecular targets for interventions are required. The malaria causative pathogen, a _Plasmodium_ parasite infection in a mosquito, is

complicated. Unlike most other pathogens, parasites in host blood are mainly asexual stage parasites in red blood cells, and some develop sexual stage parasites called gametocytes, and only

gametocytes can infect mosquitoes3. Once a female mosquito takes a meal of infectious blood, gametocytes in blood develop into ookinetes through fertilization in mosquito midguts during the

first hours after the ingestion of infectious blood4. The blood meal also stimulates the mosquito midguts to secrete proteins and other materials to form a peritrophic matrix (PM) that wraps

the blood bolus. PM is visible 4 h after a blood meal5. A sexual stage parasite attaches PM and penetrate two physical barriers, the PM and epithelium, to reach the basal lamina, where it

develops into an oocyst. During this infection process, the molecular interaction between mosquito midgut proteins and sexual stage parasites, e.g., gametocytes or ookinetes, directly

affects malaria transmission. For instance, alanyl aminopeptidase N 1 (AnAPN1)6,7, annexin protein8, and fibrinogen-related protein 1 (FREP1)9 bind to the sexual stage of _Plasmodium_

parasites and facilitate malaria transmission. Antibodies (Ab) against these mosquito midgut proteins prevent _Plasmodium_ transmission to _Anopheles_10,11. These transmission-blocking

targets share some common biochemical features, such as higher abundance in the midgut than in other tissues, the induction of expression immediately after a blood meal12, and direct access

to large molecules such as Ab in human blood. Based on these features, we chose mosquito genes that are up-regulated three hours after a blood meal, and their products have signal peptides.

Then, we examined the interaction between the candidate mosquito proteins and parasites, particularly gametocytes or ookinetes. Finally, we determined the functional relationship between

candidate mosquito proteins and _Plasmodium_ transmission to mosquitoes. Five novel proteins related to malarial transmission were discovered. These genes and their products are promising

targets for transmission-blocking vaccines, drugs, or transgenic mosquitoes for malaria control. RESULTS SELECTION OF _AN. GAMBIAE_ PROTEINS THAT POTENTIALLY AFFECT _P. FALCIPARUM_ INVASION

OF MIDGUTS Blood feeding stimulates the midguts to secrete materials to form PM, which surrounds the ingested blood, protecting mosquitoes from potential pathogens in the bloodmeal. The PM

is visible in the midgut lumen 4 h after bloodmeal and disappears 24 h after the bloodmeal5. The first few hours after ingestion are keys for gametogenesis, ookinete formation, and

transmission4. Also, gene expression data at 3 h post bloodmeal are publicly available. In order to find mosquito proteins that affect malaria transmission to mosquitoes in midguts, we used

four criteria to select candidates: 1) the proteins have signal peptides; 2) the genes express higher in the midgut than in any other tissues (> 1.2-fold); 3) the genes are up-regulated 3

 h after a blood meal (> 1.2-fold), and 4) the expression of the genes return to the regular level (naïve mosquitoes) 24 h after a blood meal. Using the four criteria, we selected the

proteins containing signal peptides from our previous annotation13 and the vectorbase (https://www.vectorbase.org)14. We used published oligo-array data15, which is also available online at

https://vectorbase.org/download/anopheles-gambiaeexpr-statsvb-2019-06txtgz, to determine gene expression. About 76 genes (Table S1) that satisfy the four criteria were chosen as candidates

for further analysis. Three previously identified midgut proteins, including FREP1 (AGAP007031), AnAPN1 (AGAP004809), and HPX15 (AGAP013327), are presented in our predicted candidate list

(Table S1). These 76 candidate proteins belong to three main functional groups: protection, digestion, and unknown (Fig. 1). About 44.7% of the candidate proteins were expected to protect

mosquitoes from infection, which included 15 immune-related proteins (20%), 16 structural proteins (21%), and 3 signal-transduction proteins (Fig. 1). Among these immune-related proteins,

HPX15 has been reported to promote malaria transmission16. The immune-related proteins also included nine pattern recognition molecules, e.g., peptidoglycan recognition protein, three

leucine-rich-repeat (LRR) proteins, and five immunoglobin E-set containing proteins. In terms of the 16 midgut structural proteins, most are involved in the formation of PM, such as

chitin-binding proteins, cadherins, and keratin-associate proteins. FREP1, a PM structure protein, has been reported to mediate malaria transmission by interacting with ookinetes9. Moreover,

a blood meal triggers midgut cells to secrete enzymes to digest blood12. Therefore, a substantial portion (48.7%) of candidate proteins are involved in the digestion process (Fig. 1), which

matches the primary function of the insect midgut17. Among them, the most common enzymes were peptidases (n = 14), including ten trypsin-like peptidases, two aminopeptidases, and three

carboxypeptidases. CANDIDATE MOSQUITO MIDGUT PROTEINS THAT INTERACT WITH _P. FALCIPARUM_ To determine these mosquito proteins that could bind to _Plasmodium_ parasites, we cloned and

expressed the candidate proteins with a baculovirus system. The amplified gene coding regions were cloned into bacmid and expressed in High Five cells using a serum-free medium to produce

the recombinant proteins. FREP1 and HPX15 were expressed in the same system as internal controls. Seven genes, including AnAPN1, were not cloned due to incorrect annotation or other reasons.

Eight genes were removed from further study because of their low expression. The remaining sixty-one genes (Fig. 2A) were expressed at the ELISA-detectable level (Fig. 2B). About 23% of the

candidate proteins were highly expressed (> 50 μg/106 cells), about 30% (18/61) were moderately expressed (> 10 μg/106 cells), and the rest were expressed at a detectable level (Fig. 

2B). Each of the recombinant protein had a 6 × His tag at its C-terminal that would be probed with anti-His monoclonal Ab. With these 61 insect cell-expressed recombinant proteins, we

examined their interaction with _P. falciparum_. The _P. falciparum_-infected cell culture contains cells in various stages: uninfected, asexual stage, and sexual stage parasites

(gametocytes and ookinetes). ELISA assays are more sensitive and easier than other methods. Thus, it was used as the screening approach to find candidate proteins that interacted with _P.

falciparum_. The 15-day cultured _P. falciparum_ that contained more than 10% gametocytes were collected by centrifugation and lysed. The lysate was used to coat the plates, followed by

blocking with BSA. The recombinant proteins were diluted, and 0.2 pmol of each recombinant protein was added to each well (Fig. 2A). The retained recombinant proteins in wells were detected

by anti-His monoclonal Ab. Recombinant FREP1 (Fig. 2C, A8) and heat-inactivated FREP1 (Fig. 2C, F8) were used as the positive and controls, respectively. The results (Table S2) showed that

A405 of the 25 proteins were over triple of the negative control (Fig. 2C, Table 1). Out of these 25 proteins, 11 proteins (44%, including FREP1) showed > tenfold of binding signals of

the negative control, and 9 proteins (36%) showed medium binding signs (≥ fivefold of the negative control;). It is worth noting that the Histidine tag does not bind to _P. falciparum_

lysate based on our previous publication9. Indeed, the heat-inactivated 6xHis FREP1 did not show any interaction signal (Fig. 2C, F8). This experiment was conducted twice, and the results

were consistent. The function of the 25 _P. falciparum_-binding proteins was summarized in Table 1. Ten out of 25 parasite-binding proteins are involved in digestion, including five

serine-type peptidases (AGAP004900, AGAP008176, AGAP005686, AGAP001245, and AGAP011920). Ten out of 25 parasite-binding proteins were related to protection, including four niemann-Pick Type

C-2 (NPC-2) proteins and three fibrinogen-related proteins (AGAP011371, FREP1, and AGAP004918). Five of 25 parasite-binding proteins were unknown. FUNCTIONAL ANALYSES OF THE CANDIDATE _AN.

GAMBIAE_ MIDGUT GENES ON _P. FALCIPARUM_ INFECTION IN MOSQUITO MIDGUTS BY RNAI For the _P. falciparum_-binding midgut proteins, we examined their effects on _P. falciparum_ infection in

mosquitoes. Because FREP112 and HPX1518 were previously examined with knockdown assays, we focused on the remaining 23 candidate genes. Gene-specific dsRNA was injected into the mosquito

hemolymph to knock down the expression of a candidate gene. Then we infected the treated mosquitoes with the cultured _P. falciparum_. Seven days after the infection, mosquitoes were

dissected to count the oocysts in the mosquito midguts. GFP dsRNA was used as a control. The number of oocysts in each mosquito of the experimental group was compared to that of the control

group by a Mann–Whitney-Wilcoxon test. The results are summarized in Table 2 and shown in Fig. 3A. Out of 23 genes, five (AGAP006268, AGAP002848, AGAP006972, AGAP008138, and AGAP002851)

showed significant effects on _P. falciparum_ transmission to mosquitoes, e.g., the number of oocysts was significantly different between the experimental group and the control group (_p_ 

< 0.05, significance score > 3). If injecting a specific dsRNA into a mosquito did not change the mosquito phenotypes of infection for some reason, such as low knockdown efficiency, we

would not analyze it in this report. To determine the knockdown efficiency, we examined the expression level of the candidate genes in the treated mosquitoes using quantitative RT-PCR 48 h

after injecting dsRNA. The results confirmed that the expression of five candidate genes was down-regulated by gene-specific dsRNA (Fig. 3B). The expression of AGAP008138 is about 40% of the

control, while only 5% of AGAP002848 mRNA was left after dsRNA injection compared to the control. Notably, knocking down either one of AGAP006268 (peritrophin), AGAP002848 (NPC-2),

AGAP006972 (keratin-associated protein 16–1), or AGAP002851 (NPC-2) significantly (_p_ < 0.05) increased the number of _P. falciparum_ oocysts in mosquito midguts compared to the control

with 1.6-folds, 2.5-fold, 4.5-fold, and 1.9-fold changes, respectively (Fig. 3C), indicating that these four proteins inhibited malaria infection in mosquitoes. However, knockdown of

AGAP008138 (uncharacterized) expression significantly decreased the number of _P. falciparum_ oocysts in _An. gambiae_, from 9.7 to 3.9 per midgut (Fig. 3C), suggesting that the AGAP008138

gene product facilitated _Plasmodium_ invasion in mosquitoes. The RNAi experiments of the five candidate genes were conducted at least twice, and the results were reproducible (Fig. 3C).

CONFIRMATION OF THE INSECT CELL-EXPRESSED CANDIDATE PROTEINS BY WESTERN BLOTTING AND THEIR INTERACTION WITH THE SEXUAL STAGE _P. FALCIPARUM_ First, we confirmed a set of insect

cell-expressed candidate proteins, including the five _P. falciparum_-transmission related proteins, which were specifically recognized by anti-His antibody using Western blotting assays.

Samples containing recombinant proteins were separated by 12% SDS-PAGE and analyzed by western blotting assays with anti-His antibodies. Seven recombinant proteins, including the five

recombinant proteins, AGAP006972, AGAP008138, AGAP002581, AGAP002848, and AGAP006268, were specifically detected by anti-His monoclonal antibodies (Fig. 4, lanes 1, 3, 4, 6, and 7,

respectively). The size of AGAP006972 protein with the signal peptide was predicted about 15 kDa, similar to the observed band (Fig. 4, lane 1). The molecular masses of the mature proteins

of AGAP008138 and AGAP002581 were expected to be 55 kDa and 16 kDa, matching the observed bands (Fig. 5, lanes 3 and 4, respectively). The predicted sizes of the precursor and mature

AGAP002428 are 18 kDa and 16 kDa, respectively, matching the observed two bands (Fig. 4, lane 6). The size of the AGAP006268 product with the signal peptide was expected to be 12 kDa,

matching the observed band (Fig. 4, lane 7). The data supported that the recombinant proteins were expressed by the insect cells in full length with or without signal peptides. This data

also demonstrated that the anti-His monoclonal antibody is specific, and the ELISA signals in Fig. 2C corresponded to the bound recombinant proteins. Next, an indirect fluorescence assay

(IFA) was used to examine the interaction between a candidate protein and sexual stage _P. falciparum_ parasites, because only sexual stage parasites are relevant to parasite infection in

mosquito midguts. Following our previous published methods to generate ookinetes9,19, we collected the late-stage cultured _P. falciparum_ cells and suspended them in an ookinete culture

medium and incubated for 16 h at RT. The cell mixture, containing uninfected cells, asexual stage (trophozoites and schizont), and sexual stage parasites (gametocytes and ookinetes), were

deposited onto glass coverslips and fixed with 4% paraformaldehyde. These cells were then incubated sequentially with the insect cell-expressed candidate protein and detected by

fluorenes-labeled antibodies. The unrelated insect cell-expressed protein chloramphenicol acetyltransferase (CAT) with 6 × His tag at its C-terminal was used as a negative control. We also

added anti-His antibody directly immediately after blocking to confirm the anti-His antibodies did not bind to cells as a negative control. Under a fluorescent microscope, the bound

recombinant proteins that were recognized by the anti-His Ab showed a red color, and cell nuclei of parasites appeared blue. Sexual stage and asexual stage parasites were different in shape.

The results (Fig S1) showed 24 candidate proteins bound to sexual and asexual _P. falciparum_ at various levels. After subtracting the background pixel intensity, we found that all of the

ELISA-positive proteins interacted with _P. falciparum_-infected cells (Fig S1A). Out of 24 proteins (FREP1 was excluded), 17 interacted with sexual parasites significantly stronger than

asexual stage parasites (Fig S1C). For the five candidate proteins that are related to parasite transmission (Fig. 5A), we found that they significantly bound more to sexual _P. falciparum_

parasites than to asexual stage parasites (Fig. 5B, _p_ < 0.05). In addition, the interaction signals to sexual stage parasites were also significantly stronger than the negative control

(Fig. 5C, _p_ < 0.05). It is worth noting that both gametocytes and ookinetes are relevant to malaria transmission. Therefore, we did not distinguish one over the other at this time.

Together, our Western blotting data and IFA demonstrated that the five candidate proteins specifically interacted with _Plasmodium_ sexual stage parasites (gametocytes and/or ookinetes).

DISCUSSION It is essential for _Plasmodium_ parasites to infect mosquitoes to complete a malaria transmission cycle. After a blood meal, mosquito midgut epithelial cells secrete materials

such as proteins, chitin to form PM, which wraps blood bolus inside. Thus, parasites must overcome the PM first to infect mosquitoes. Only the molecules inside or within PM can contact large

molecules such as Ab in the host blood9. To identify some midgut proteins that are important for malaria transmission, we focused on proteins that were highly expressed and secreted in

midguts and up-regulated three hours post blood meal. We selected these genes as our candidates for further analysis. Consistent with our expectations, functional analysis in our results

revealed that most of these candidate proteins were involved in PM formation, microbe recognition, and digestion. We successfully PCR-cloned and insect cell-expressed > 80% of the

candidates. Nearly half (41%) of candidate proteins bound to the cultured _P. falciparum_ parasite lysate. The detailed analysis found two known proteins, FREP1 and HPX15, bound to

parasites, which was consistent with the previous report9,16. They participated in _Plasmodium_ transmission to mosquitoes. Seven parasite-binding proteins were peptidases or proteases that

should bind to the substrate proteins. Four NPC-2 proteins bound to _P. falciparum_ lysates. Two NPC-2 proteins (AGAP002848 and AGAP002851) were immunoglobulin-like secreted proteins,

containing MD-2-related lipid-recognition (ML) domain. ML domain is conserved from fungi to plants and animals20. NPC-2 proteins play important roles in lipid metabolism and immune signaling

pathway21. NPC-2 proteins were named because its identification is related to Niemann-Pick disease type C2. In invertebrates, evidence shows that NPC-2 proteins may be involved in innate

immune signaling pathways, such as the immune deficiency (Imd) pathway. Three Drosophila NPC2 proteins can bind the bacterial cell wall components of peptidoglycan (PG) and lipoteichoic acid

(LTA) to serve as a pathogen recognition receptor (PRR) to initiate the innate immune responses22,23,24. The 25 proteins that bound to the _Plasmodium_-infected cell lysate also included

six unknown proteins. Indeed, all the 25 candidate proteins were confirmed to bind to _P. falciparum_-infected cells by IFA assays. About 68% of these proteins showed significantly higher

interaction signals with sexual stage parasites than asexual stage parasites. Since both gametocytes and ookinetes are related to malaria transmission, we did not distinguish them in this

report. Among the 25 parasite-binding proteins, 28% (n = 7) significantly affected parasite transmission when the gene expression was knocked down. Two (HPX15 and FREP1) were previously

known, and five genes had not been reported as related to _Plasmodium_ transmission. By silencing any of AGAP006268 (peritrophin), AGAP006972, AGAP002848 (NPC-2 related), or AGAP002851

(NPC-2 related), the number of _P. falciparum_ oocysts in mosquito midguts significantly increased compared to that of the control, suggesting they protected mosquitoes from infection.

Specifically, AGAP006268 is a structural protein that is secreted by _Anopheles_ midgut epithelial cells after a blood meal to form PM. It contains a chitin-binding domain at its

N-terminus25, which allows peritrophin to bind to chitin fibers and form the structural basis of PM26. Reducing peritrophin expression might destroy the integrity of PM, and facilitates

parasite penetration of PM. The function of AGAP006972 is unknown. There are several repeats in the 96-amino acid AGAP006972 peptide, including eight GGY repeats and three GGF. The two NPC-2

proteins (AGAP002848 and AGAP002851) may serve as a PRR to trigger the innate immune response. Different from the other four genes, but similar to FREP1, knocking down the expression of

AGAP008138 significantly reduced the number of _P. falciparum_ oocysts in the mosquito midguts. The mature AGAP008138 has 492 amino acids in length. However, AGAP008138 does not have any

known motifs. Its expression was induced 7.5-fold 3 h post-blood meal and decreased close to or less than naive mosquitoes after 24 h. IFA showed that it bound to ookinetes. Notably,

AGAP008138 is a unique gene, existing only in the _Anopheles_ species and not in _Aedes_ or _Culex,_ which is consistent with the fact that only _Anopheles_ mosquitoes transmit malaria.

Therefore, AGAP008138 might be an ideal target for malaria transmission-blocking vaccine development, although the detailed mechanisms of these mosquito genes on parasite transmission need

further investigation. This study identified a set of new midgut proteins that interact with _P. falciparum_ ookinetes and affect malaria transmission. Further investigation of these

proteins will help to elucidate the molecular mechanisms of a _Plasmodium_ invasion in mosquito midguts as well as the innate immunity in mosquitoes. MATERIALS AND METHODS REAR MOSQUITOES

_An. gambiae_ (G3 strain) eggs were obtained from BEI Resources (https://www.beiresources.org/). The insectary was set at 27 °C, 80% relative humidity, 12-day/night cycles. Larvae were fed

with grounded fish food, and adult mosquitoes were maintained with 8% sucrose solution. CULTURE _P. FALCIPARUM_ GAMETOCYTES AND OOKINETES _P. falciparum_ (NF54) was obtained from BEI

Resources, and cultured with RPMI-1640 complete medium, containing 4% new O+ human red blood cells, 10% human AB + serum, and 12.5 μg/mL of hypoxanthine in a candle-jar at 37 °C. The culture

started at 0.1% parasitemia, and day 15–17 _P. falciparum_ cultures were used to infect the mosquitoes or to be lysed, as described previously9. To prepare _P. falciparum_ ookinetes, we

transferred 5 mL of day-15 cultured _P. falciparum_ containing ~ 2% stage V gametocytes into a 15 mL centrifuge tube and centrifuged at room temperature at 650 g for 5 min (m). The pellet

was washed with RPMI-1640 three times and then resuspended in 500 µL of sterile ookinete culture medium (RPMI-1640, 20% human serum AB+, 50 µg/mL of hypoxanthine, 2 g/L NaHCO3). The

resuspended cells were transferred into a well of a 12-well plate and incubated at room temperature on a shaker (50 rpm) for 24 h to generate ookinetes. Finally, the cell mixtures of the

ookinetes, gametocytes and the asexual-stage _P. falciparum_ were collected by centrifugation at 650 g for 5 m at RT. SELECTION OF CANDIDATE _AN. GAMBIAE_ MIDGUT PROTEINS We focused on the

proteins that may contact parasites directly in mosquito midgut lumen. PM starts to form inside of the midgut from secretory materials immediately after a blood meal, reaching its thickest

18 h post blood meal. Therefore, we chose the proteins that have signal peptides, and the corresponding genes are immediately up-regulated by a blood meal, return to regular expression at 24

 h after a blood meal, and expressed higher in midguts than in other tissues. The candidate _An. gambiae_ midgut proteins were selected from the databases of ReAnoXcel13, vectorbase14, and

AgExpression15 according to the following four criteria: (1) candidate proteins contain N-terminal signal peptides based on _An. gambiae_ protein databases from previous annotation13 and the

vectorbase (https://www.vectorbase.org)14 ; (2) the expression of candidate genes is > 1.2-fold higher 3 h post bloodmeal comparing to naïve mosquitoes; (3) the expression of candidate

genes should return to the expression level of naïve mosquitoes 24 h post-bloodmeal; and (4) candidate genes are expressed > 1.2-fold higher in midguts than in other tissues. The gene

expression data were obtained from the vectorbase database (https://vectorbase.org/download/anopheles-gambiaeexpr-statsvb-2019-06txtgz) and AgExpression of a previous publication15. The

protein functions were obtained from our previous annotation13 and the uniprot database (https://www.uniprot.org). CLONING AND EXPRESSION OF CANDIDATE GENES IN INSECT CELLS Total RNA was

extracted from adult female mosquito midguts using RNAzol (Sigma-Aldrich, MO). About 20 midguts were dissected from three-day-old female _An._ gambiae and put into a 1.5 mL plastic tube that

contained 200 μl of RNAzol. The tissue was grounded by a micro pestle (Sigma-Aldrich). Eight hundred µL RNAzol was added. After centrifugation (13,000 g for 10 m), RNA in the supernatant

was precipitated by isopropanol (1:1 v/v). The cDNA was synthesized with the Superscript First-Strand Synthesis System (Invitrogen, CA). Gene fragments were amplified by PCR using the DNA

Engine Dyad Thermal Cycler (Bio-Rad, CA) with gene-specific primers (Table S3). DNA fragments were purified through a GeneJet PCR Purification Kit (Thermo Fisher Scientific) and cloned into

modified donor plasmid pFastBac1 that contains a 6 × His tag at the C-terminal of multiple cloning sites. Recombinant plasmids were transformed into competent DH5α cells. Positive

recombinant plasmids were confirmed by sequencing and then transformed into DH10Bac to obtain recombinant Bacmids that showed white colonies on culture plates. The positive white colonies

were picked and confirmed by PCR. One μg of a recombinant Bacmid in 100 μL un-supplemented grace medium (Invitrogen) was mixed with 100 μL of un-supplemented Gibico grace medium (Thermal

Fisher Scientific), containing 5 μl of cellfection II (Thermal Fisher Sci), and incubated for 30 m at RT. The mixture was then added into 1.5 mL of unsupplemented grace medium that contained

800,000 sf9 cells in a well of a six-well culture plate and incubated for 4 h at 26 °C. The supernatant was replaced with 2 mL of complete race medium with 10% FBS (Invitrogen) and

incubated at 26 ºC for three days to generate recombinant baculovirus viruses. A 100 μL culture supernatant that contained the recombinant virions were added in 2 mL of Express Five SFM

medium (Gibco) supplemented with 20 mM of L-glutamine and 1 million High Five cells in a well of a six-well culture plate. The cells were incubated at 26 ºC for three days to express the

recombinant proteins of interest. The recombinant proteins were quantified with anti-His monoclonal Ab using ELISA plates twice. ANALYSIS OF THE INTERACTION BETWEEN A RECOMBINANT PROTEIN AND

THE _P. FALCIPARUM_ LYSATE USING ELISA Day 15 cultured _P. falciparum_ (containing > 10% stage IV and V gametocytes) were harvested by centrifugation (300 g for 5 m). The cells were

lysed in native cell lysis buffer (Clontech) and ultra-sonication for 2 m on ice, pausing for 50 s (s) every 10 s of sonication. Cell debris was removed by centrifugation (13,000 g for 5 m),

and the protein concentration in the supernatant was measured with the Bradford method. About 50 µL of 3 µg/mL _P. falciparum_ parasite protein was added per well on a 96-well plate and

incubated at 4 ºC overnight. The plate was blocked with 100 µL of 2% BSA for 2 h at room temperature; then, 0.2 pM recombinant candidate proteins in 50 µL PBS were added to each well and

incubated for 1 h. Then, 50 µL of anti-His mouse monoclonal Ab (Sigma, 1:3,000 dilution with blocking buffer) was added to detect the recombinant protein and incubate at RT for 1 h, followed

by incubation with 50 µL of goat anti-mouse IgG-Alkaline Phosphatase conjugate (Sigma, 1:10,000 dilution in blocking buffer ) for 1 h. The plate was washed three times with PBST between

incubations. Finally, 50 µL of _p_-nitrophenyl phosphate (pNPP) (Sigma-Aldrich, MO) was added for development, and the A405 was measured. The insect cell-expressed FREP1 recombinant protein

was incubated for 30 m at 65 °C to inactivate FREP1. The heat-inactivated FREP1 was used as a negative control because it did not bind to the parasites, as reported previously19.

DETERMINATION OF THE EFFECTS OF _AN. GAMBIAE_ GENES ON _P. FALCIPARUM_ TRANSMISSION TO MOSQUITOES USING DSRNA MEDIATED GENE EXPRESSION SILENCING ASSAYS The procedure was similar to the

previous report27. Gene-specific primers that contained a T7 promoter and candidate gene sequences were designed with the E-RNAi web server28. Double-stranded RNAs (dsRNA) were synthesized

with a MEGAscript Kit (Thermo Fisher Scientific, MA). GFP dsRNA was used as a control. About 67 nl of 3 µg/µL dsRNA was injected into 1-day old _An. gambiae_ hemocoel using Nanoject II

(Drummond Scientific, PA), and 100 mosquitoes were injected for each candidate gene. Thirty-six hours after treatment, the treated mosquitoes were fed with 0.2% _P. falciparum_

gametocytaemia. The midguts of the infected mosquitoes were dissected seven days post-infection and stained with 0.1% mercury dibromofluorescein disodium in PBS. Oocysts were counted under a

light microscope. The Mann–Whitney-Wilcoxon test was used to determine the statistical difference of the oocysts between the experimental group and the control. The transcript knockdown

efficiency was confirmed by the quantitative RT-PCR in five of treated mosquitoes that were taken randomly 24 h after infection. GelDoc (UVP, Upland, CA) was used to take photos of the gels.

PCR reactions (to detect specific genes and loading control S7) from one sample were run in the same gel. The bands from a particular gene and S7 were cropped for easier interpretation and

smaller file sizes. DETECTION OF RECOMBINANT PROTEINS WITH A MONOCLONAL ANTI-HIS ANTIBODY WITH WESTERN BLOTTING To maximize the yield of recombinant proteins, we collected the expressed

proteins in both culture medium and cells. The culture cell medium was separated by centrifugation (650 g for 4 m). The supernatant was concentrated 100-fold by Centricon 10 Centrifugal

Filter Device (Millipore, Danvers, MA). The cell pellets were lysed in native cell lysis buffer (Clontech). The cell lysis and concentrated medium were mixed and loaded on 12% SDS-PAGE and

separated by electrophoresis until the Bromophenol blue reached the bottom. The proteins on the gel were then transferred on nitrocellulose membrane. The membrane was blocked with 2% BSA in

PBST, followed by incubation in mouse monoclonal anti-His antibody (Sigma, 1:2000 dilution with blocking buffer) at RT for 1 h, goat anti-mouse IgG-alkaline phosphatase conjugate (Sigma,

1:10,000 dilution in blocking buffer ) for 1 h. The membrane was washed three times with PBST between incubations. Finally, nitroblue tetrazolium (Sigma-Aldrich, MO) was added to develop.

INDIRECT IMMUNOFLUORESCENCE ASSAYS TO DETERMINE MIDGUT PROTEIN-OOKINETE INTERACTIONS The cultured _P. falciparum_ gametocytes with ookinetes were deposited onto premium cover glass slips to

make blood smears. Before the smears were completely dry, the semi-dry smears were fixed in 4% paraformaldehyde in PBS for 30 m at RT to keep the cell membrane intact. Then, the cover glass

slips were sequentially incubated in 1 mL of PBS that contained 10 mM glycine for 20 m and the blocking buffer for 1.5 h at RT. After blocking, cells on a coverslip were incubated with a

candidate recombinant protein (10 μg/mL) in 200 μl of PBS containing 0.2% BSA (blocking buffer) for 1 h, followed by sequential incubation with 4 drops of enhancer (Alexa Fluor ® 594 Goat

Anti-mouse SFX kit, Invitrogen) for 30 m, 200 μl of anti-His monoclonal Ab (1:2,500 dilution in the blocking buffer, 2 μg/mL) for 1 h, and 200 μl of secondary Ab (Alexa Fluor ® 594 Goat

Anti-mouse SFX kit, Invitrogen; 1:1,000 dilution in the blocking buffer) for 30 m. Between each incubation, the slip was washed three times with 1 mL of blocking buffer for 3 m each. In the

end, the coverslip was rinsed in distilled water for 20 s, and then coated with 4,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). About 20 μl of VECTASHIELD anti-fade mounting media

(Vector Laboratories, Burlingame, CA) was added onto the coverslip and mounted onto a slide. After incubation for at least 2 h in the dark, the cells were examined under fluorescence

microscopy (Nikon Eclipse Ti-S fluorescence microscope). An unrelated protein chloramphenicol acetyltransferase was used to replace a candidate protein as a negative control, as demonstrated

previously19. We also added anti-His antibody directly immediately after blocking to confirm the anti-His antibodies did not bind to cells as a negative control. To quantify the binding

proteins, we measured the red pixel intensity values of the parasites at three randomly selected regions using Adobe Photoshop (version 2018, San Jose, CA). All values subtracted the

background value. Then, we calculated the difference of the mean red pixel intensity values between sexual parasites and asexual parasites or the negative control and experimental groups.

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references ACKNOWLEDGEMENTS This study was supported by NIAID 1R01AI125657 and NSF Career Award 1453287, 1742644 to JL. The funders had no role in the study design, data collection, and

analysis, decision to publish, or preparation of the manuscript. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biological Sciences, Biomolecular Sciences Institute, Florida

International University, 11200 SW 8th Street, Miami, FL, 33199, USA Yingjun Cui, Guodong Niu, Vincent L. Li, Xiaohong Wang & Jun Li Authors * Yingjun Cui View author publications You

can also search for this author inPubMed Google Scholar * Guodong Niu View author publications You can also search for this author inPubMed Google Scholar * Vincent L. Li View author

publications You can also search for this author inPubMed Google Scholar * Xiaohong Wang View author publications You can also search for this author inPubMed Google Scholar * Jun Li View

author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS Y.C. cloned and expressed the genes, and conducted the protein-parasite interaction and RNAi

assay. G.N. setup the expression system, expressed some genes and confirmed interaction assays. X.W. and V.L.L. conducted the bioinformatics and statistical data analysis. Y.C., G.N., and

J.L. wrote the paper. V.L.L. participated in the data analysis and proofread the manuscript. J.L. conceived the concept and designed the study. CORRESPONDING AUTHOR Correspondence to Jun Li.

ETHICS DECLARATIONS COMPETING INTERESTS The authors have no competing interests as defined by Nature Research, or other interests that might be perceived to influence the results and/or

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copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Cui, Y., Niu, G., Li, V.L. _et al._ Analysis of

blood-induced _Anopheles gambiae_ midgut proteins and sexual stage _Plasmodium falciparum_ interaction reveals mosquito genes important for malaria transmission. _Sci Rep_ 10, 14316 (2020).

https://doi.org/10.1038/s41598-020-71186-5 Download citation * Received: 19 September 2019 * Accepted: 11 August 2020 * Published: 31 August 2020 * DOI:

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