Anti-microbial efficacy of l-glutaminase (ec 3. 5. 1. 2) against multidrug-resistant pseudomonas aeruginosa infection

ABSTRACT The aims of this study were isolation-purification and characterization of l-glutaminase from _L. gasseri_ BRLHM clinical isolates and investigation of its efficiency as an

antimicrobial agent against multidrug-resistant _P. aeruginosa_. The MICs of l-glutaminase and gentamicin reference were evaluated by the well-diffusion method. The biofilm on the IUD

contraceptive was visualized using atomic force microscopy (AFM) image analyses. The purified l-glutaminase possessed significant antimicrobial activity against _P. aeruginosa_ isolates (_p_

 < 0.05), and the antibiofilm formation activity of the purified l-glutaminase was stronger than the antibiofilm activity of the referral standard drug, gentamicin (_P_ < 0.05), which

were checked by the inhibition of the biofilm formation on the IUD contraceptive device. Investigations indicated that l-glutaminase may have a crucial role in future clinical applications.

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thank the Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq; and the Head and staff members of the Division of Biotechnology, Department of Applied Sciences,

University of Technology, and Dean College of Pharmacy, and Head Department of Medicinal Chemistry, College of Pharmacy, Qassim University, Saudi Arabia for support and encouragement during

the course of the study. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq Likaa H. Mahdi & Rajwa H. Essa

* Division of Biotechnology, Department of Applied Sciences, University of Technology, Baghdad, Iraq Buthenia A. Hasoon & Ghassan M. Sulaiman * Department of Medicinal Chemistry and

Pharmacognosy, College of Pharmacy, Qassim University, Qassim, 51452, Saudi Arabia Hamdoon A. Mohammed * Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar

University, Cairo, Egypt Hamdoon A. Mohammed * Department of LASER and Optoelectronic Engineering, University of Technology, Baghdad, Iraq Kareem H. Jawad * Department of Dentistry, Bilad

Alrafidain University College, Diyala, 32001, Iraq Ali G. Al-Dulimi * Department of Chemistry, College of Science, University of Misan, Maysan, 62001, Iraq Salim Albukhaty * College of

Medicine, University of Warith Al-Anbiyaa, Karbala, Iraq Salim Albukhaty * Manav Rachna International Institute of Research and Study (MRIIRS), Faridabad, HR, 121 001, India Riaz Khan

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can also search for this author inPubMed Google Scholar * Kareem H. Jawad View author publications You can also search for this author inPubMed Google Scholar * Ali G. Al-Dulimi View author

publications You can also search for this author inPubMed Google Scholar * Rajwa H. Essa View author publications You can also search for this author inPubMed Google Scholar * Salim

Albukhaty View author publications You can also search for this author inPubMed Google Scholar * Riaz Khan View author publications You can also search for this author inPubMed Google

Scholar CORRESPONDING AUTHORS Correspondence to Ghassan M. Sulaiman, Hamdoon A. Mohammed or Riaz Khan. ETHICS DECLARATIONS CONFLICT OF INTEREST The authors declare no competing interests.

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Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Mahdi, L.H., Hasoon, B.A., Sulaiman, G.M. _et al._ Anti-microbial efficacy of l-glutaminase (EC 3.5.1.2) against

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