The structural basis of blebbistatin inhibition and specificity for myosin ii

ABSTRACT Molecular motors play a central role in cytoskeletal-mediated cellular processes and thus present an excellent target for cellular control by pharmacological agents. Yet very few

such compounds have been found. We report here the structure of blebbistatin, which inhibits specific myosin isoforms, bound to the motor domain of _Dictyostelium discoideum_ myosin II. This

reveals the structural basis for its specificity and provides insight into the development of new agents. Access through your institution Buy or subscribe This is a preview of subscription

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helpful discussions and C. Grutzmacher for technical assistance. This work was supported by a fellowship to J.S.A. from the Canadian Institutes of Health Research (64606) and a research

grant to I.R. from the US National Institutes of Health (AR35186). Use of the LS-CAT beamline at Argonne National Laboratory Advanced Photon Source was supported by the US Department of

Energy, Office of Energy Research, under contract no. W-31-109-ENG-38. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biochemistry, University of Wisconsin, 433 Babcock Drive,

Madison, 53706, Wisconsin, USA John S Allingham, Robert Smith & Ivan Rayment Authors * John S Allingham View author publications You can also search for this author inPubMed Google

Scholar * Robert Smith View author publications You can also search for this author inPubMed Google Scholar * Ivan Rayment View author publications You can also search for this author

inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Ivan Rayment. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY

INFORMATION SUPPLEMENTARY FIG. 1 Stereo view of electron density for blebbistatin bound to _Dictyostelium discoideum_ myosin S1dC. (PDF 625 kb) SUPPLEMENTARY FIG. 2 Stereo view of apo and

blebbistatin-bound forms of S1dC. (PDF 710 kb) SUPPLEMENTARY FIG. 3 Inhibition of S1dC by blebbistatin. (PDF 57 kb) SUPPLEMENTARY TABLE 1 Data collection and refinement statistics for the

structural analysis. (PDF 71 kb) SUPPLEMENTARY METHODS (PDF 39 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Allingham, J., Smith, R. &

Rayment, I. The structural basis of blebbistatin inhibition and specificity for myosin II. _Nat Struct Mol Biol_ 12, 378–379 (2005). https://doi.org/10.1038/nsmb908 Download citation *

Received: 21 December 2004 * Accepted: 01 February 2005 * Published: 06 March 2005 * Issue Date: 01 April 2005 * DOI: https://doi.org/10.1038/nsmb908 SHARE THIS ARTICLE Anyone you share the

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