Monkey mutants with TALENs | Nature Methods

You have full access to this article via your institution. Download PDF Liu, H. _et al_. _Cell Stem Cell_ 14, 323–328 (2014). Models of human disease in nonhuman primates are of high

interest in biomedicine, and targeted genome-editing technologies offer a way to realize this goal. In recent work, Liu _et al_. demonstrated the use of transcription activator–like effector

nucleases (TALENs) to mutate the _MECP2_ gene in rhesus and cynomolgus macaques and thus to generate a primate model of Rett syndrome, an X-linked neurodevelopmental disease. The

researchers used three TALEN pairs targeting the gene at different positions and delivered the nucleases to one-cell zygotes as plasmid DNA. They were able to efficiently modify the gene in

embryos and did not detect any off-target effects. After implantation, male fetuses carrying _MECP2_ mutations were miscarried, as in humans. Female mutant monkeys were carried to term and,

at the time of publication, had not yet presented a phenotype. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Monkey mutants with TALENs. _Nat Methods_

11, 476 (2014). https://doi.org/10.1038/nmeth.2947 Download citation * Published: 29 April 2014 * Issue Date: May 2014 * DOI: https://doi.org/10.1038/nmeth.2947 SHARE THIS ARTICLE Anyone you

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