Expression profiling of soft tissue sarcomas

Access through your institution Buy or subscribe Soft tissue sarcomas involve the connective tissues of the limbs, girdle, trunk, head and neck. Despite the numerous subtypes of sarcomas

(synovial sarcoma, fibrosarcoma and malignant fibrous histiocytoma), tumours in a particular subtype behave in a reasonably consistent fashion, allowing predictions of recurrence and

metastasis. Histological and cytogenetic techniques can group most sarcomas; however, the diagnosis can be problematic and must be performed by experts. We are using DNA microarrays to

analyse the expression pattern of genes in soft tissue to identify expression signatures specific to the various sarcoma subtypes. Our DNA expression arrays are based on cDNAs from the

UniGene set of ESTs. PCR products from an initial set of 3740 cDNAs were gridded onto poly-L-lysine-coated glass microscope slides at a density of 378 spots/cm2. Hybridisations were

performed using Cy3- and Cy5-labelled first strand cDNA from cell lines or sarcomas. After washing, the arrays were scanned using a General Scanning Scanarray3000 scanner. The results of

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AUTHORS AND AFFILIATIONS * Molecular Carcinogenesis, Institute of Cancer Research, Sutton, SM2 5NG, UK Karine Maillard, Karen Barker, Keith Aubin, Melanie Edema, Cyril Fisher & Richard

Wooster * Department of Histopathology, Royal Marsden NHS Trust, London, SW3 6JJ, UK Karine Maillard, Karen Barker, Keith Aubin, Melanie Edema, Cyril Fisher & Richard Wooster Authors *

Karine Maillard View author publications You can also search for this author inPubMed Google Scholar * Karen Barker View author publications You can also search for this author inPubMed 

Google Scholar * Keith Aubin View author publications You can also search for this author inPubMed Google Scholar * Melanie Edema View author publications You can also search for this author

inPubMed Google Scholar * Cyril Fisher View author publications You can also search for this author inPubMed Google Scholar * Richard Wooster View author publications You can also search

for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Maillard, K., Barker, K., Aubin, K. _et al._ Expression profiling

of soft tissue sarcomas. _Nat Genet_ 23 (Suppl 3), 61 (1999). https://doi.org/10.1038/14361 Download citation * Issue Date: November 1999 * DOI: https://doi.org/10.1038/14361 SHARE THIS

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