Efficient epitope mapping by bacteriophage λ surface display

ABSTRACT A bacteriophage λ surface expression system, λfoo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their

efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase I to make random epitope libraries. The libraries were screened by affinity selection using a microtiter plate

coated with monoclonal antibodies. Direct DNA sequencing of the selected clones defined two distinct epitope sites consisting of nine and 11 amino-acid residues. Affinity selection of

random peptide libraries recovered a number of sequences that were similar to each other but distinct from the galectin-3 sequence. These results demonstrate that a single affinity selection

of epitope libraries with antibodies is able to define an epitope determinant as small as nine residues long and is more efficient in epitope mapping than random peptide libraries. Access

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sequences of the M13mp18 and pUC19 vectors. _Gene_ 33: 103–119. Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * Y. Gi Mikawa Present address: The

Molecular Sciences Institute, 9894 Genesee Ave., 3rd Floor, La Jolla, CA, 92037 * Riaz I. Zuberi & Fu-Tong Liu Present address: La Jolla Institute for Allergy and Immunology, 10355

Science Center Dr., San Diego, CA, 92121 AUTHORS AND AFFILIATIONS * Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA, 92037 Ichiro

Kuwabara, Hiroko Maruyama & Ichi N. Maruyama * Departments of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Rd., La Jolla, CA, 92037 Riaz

I. Zuberi & Fu-Tong Liu Authors * Ichiro Kuwabara View author publications You can also search for this author inPubMed Google Scholar * Hiroko Maruyama View author publications You can

also search for this author inPubMed Google Scholar * Y. Gi Mikawa View author publications You can also search for this author inPubMed Google Scholar * Riaz I. Zuberi View author

publications You can also search for this author inPubMed Google Scholar * Fu-Tong Liu View author publications You can also search for this author inPubMed Google Scholar * Ichi N. Maruyama

View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Ichi N. Maruyama. RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Kuwabara, I., Maruyama, H., Mikawa, Y. _et al._ Efficient epitope mapping by bacteriophage λ surface display. _Nat Biotechnol_ 15, 74–78 (1997).

https://doi.org/10.1038/nbt0197-74 Download citation * Received: 30 September 1996 * Accepted: 07 November 1996 * Issue Date: 01 January 1997 * DOI: https://doi.org/10.1038/nbt0197-74 SHARE

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