Inter- and intraspecific heterochromatin variation detected by restriction endonuclease digestion in two sibling species of the anopheles maculipennis complex

ABSTRACT The sibling species _Anopheles atroparvus_ and _Anopheles labranchiae_ are cytogenetically almost indistinguishable. The chromosome complement (2n = 6) consists of two pairs of

autosomes and two heteromorphic sex chromosomes with largely homologous heterochromatic long arms. Treatment of chromosome preparations with the restriction endonucleases, Alu I, Hae III,

Mbo I, Hpa II, revealed species-specific differences of the sex chromosome banding pattern. These differences involved both amount and location of digested heterochromatin. Heterochromatin

heterogeneity and a high level of intraspecific polymorphism, undetected with standard banding techniques, were observed in both species. Quantitative heterochromatin differences between the

sex chromosomes did not inhibit their pairing and chiasmata formation. The endonuclease Msp I, which cleaves the same target sequence as Hpa II, did not digest heterochromatic as well as

euchromatic regions in both species: inhibition of cleavage by methylation of the target sequence or limited access of the enzyme to the target could be involved in this response. SIMILAR

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Ashburner, M. (eds) _Insect Cytogenetics_, Blackwell Sci. Publ., Oxford, pp. 245–274. Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Istituto di Biologia

Generale, Facoltà di Medicina e Chirurgia, Università di Cagliari, Via Ospedale 119, Cagliari, Sardinia, Italy, 09124 Annalisa Marchi & Roberto Mezzanotte Authors * Annalisa Marchi View

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RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Marchi, A., Mezzanotte, R. Inter- and intraspecific heterochromatin variation detected by restriction

endonuclease digestion in two sibling species of the _Anopheles maculipennis_ complex. _Heredity_ 65, 135–142 (1990). https://doi.org/10.1038/hdy.1990.80 Download citation * Received: 08

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