Proliferative behaviour of an oestrogen sensitive rat mammary tumour: evidence for a paracrine interaction between tumour and stroma

ABSTRACT An oestrogen-sensitive rat mammary tumour (OES HR1) has been grown in normal female rats and in female and male rats supplemented with oestrone. In some rats, after the tumour was

established, both exogenous and endogenous sources of oestrogen were removed--a treatment which inhibited further growth of the tumour. The proliferative characteristics of the tumours were

measured by injecting the rats with deoxybromouridine (BrdU) 4 h before removing the tumour. Extracted nuclei were reacted with anti-BrdU and the labelling index and DNA content measured by

flow cytometry. A correlation between the number of (diploid) host cells present and the number of (aneuploid) tumour cells in S-phase of the cell cycle was observed. This result suggests

that there are paracrine interactions between tumour and host cells. We also observed that, on oestrogen ablation, the labelling index was significantly reduced while the percentage of cells

in S-phase changed far less. The demonstration that there are cells in S-phase which are not proliferating highlights a possible problem with the measurement of proliferation in human

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our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS ESTROGEN-INDUCED IMMUNE CHANGES WITHIN THE NORMAL MAMMARY GLAND Article Open access 08 November 2022 ELEVATED EDAR

SIGNALLING PROMOTES MAMMARY GLAND TUMOURIGENESIS WITH SQUAMOUS METAPLASIA Article Open access 16 December 2021 DEVELOPING OVINE MAMMARY TERMINAL DUCT LOBULAR UNITS HAVE A DYNAMIC MUCOSAL AND

STROMAL IMMUNE MICROENVIRONMENT Article Open access 20 August 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Section of Cell and Experimental Pathology, Institute of Cancer Research,

Sutton, Surrey, UK MG Ormerod Authors * MG Ormerod View author publications You can also search for this author inPubMed Google Scholar * JC Titley View author publications You can also

search for this author inPubMed Google Scholar * TAD Smith View author publications You can also search for this author inPubMed Google Scholar * AL Tombs View author publications You can

also search for this author inPubMed Google Scholar * S Eccles View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Ormerod, M., Titley, J., Smith, T. _et al._ Proliferative behaviour of an oestrogen sensitive rat mammary tumour: evidence for a paracrine

interaction between tumour and stroma. _Br J Cancer_ 67, 107–111 (1993). https://doi.org/10.1038/bjc.1993.18 Download citation * Issue Date: 01 January 1993 * DOI:

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