Mitochondrial mutations in acute leukaemia

Access through your institution Buy or subscribe TO THE EDITOR Mutations in the mitochondrial genome have been reported in various forms of carcinoma. For acute leukaemia, however, there is

only one definitive report1 of their presence in one patient with acute lymphoblastic leukaemia (ALL), together with another report2 on alteration of restriction fragment length

polymorphisms in ALL cells, which could be interpreted as being due to acquired mutations. We therefore sought mitochondrial mutations in 22 patients with acute myeloid leukaemia (AML) (11

males, 11 females, ages 16–69) and 26 patients with ALL (14 males, 12 females, 23 children, three adults). In all, 15 of the ALL patients were specifically selected because they had

relapsed. All patients were studied by sequencing the mitochondrial D loop from nucleotides 16111–190 and in 37 patients; the same region was also studied by denaturing gradient gel

electrophoresis (DGGE). Mutations were found at diagnosis by sequencing in eight of the 22 patients (36%) with AML and 15 of the 26 patients (58%) with ALL. The difference in frequency

between AML and ALL was not significant (_P_=0.09, Fisher's exact test). Aberrant bands resulting from mutation were detected by DGGE with a sensitivity of 93% and a specificity of 98%.

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Department of Haematology and Genetic Pathology, Flinders University and Flinders Medical Centre, Bedford Park, South Australia, Australia S A Grist, X-J Lu & A A Morley * HuaXi

Hospital, Sichuan University, Cheng Du, Sichuan, China X-J Lu Authors * S A Grist View author publications You can also search for this author inPubMed Google Scholar * X-J Lu View author

publications You can also search for this author inPubMed Google Scholar * A A Morley View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING

AUTHOR Correspondence to A A Morley. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Grist, S., Lu, XJ. & Morley, A. Mitochondrial mutations in acute

leukaemia. _Leukemia_ 18, 1313–1316 (2004). https://doi.org/10.1038/sj.leu.2403380 Download citation * Received: 16 November 2003 * Accepted: 16 March 2004 * Published: 06 May 2004 * Issue

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