Caution in the use of liquid propane for freezing biological specimens

ABSTRACT ONE of the recent innovations in freezing and drying technique is the use of liquid propane for rapid freezing. This material has explosive possibilities when cooled in liquid

nitrogen that many invostigators do not seem to realize. Liquid nitrogen boils at − 195.8° C, liquid oxygen at − 183.0° C. It is common experience that if a vapour trap is cooled with liquid

nitrogen and open to the atmosphere, oxygen will condense in it. Since propane molts at − 189.9° C, in the range of temperatures between its melting point and the boiling point of oxygen it

is theoretically possible to get a mixture of liquid oxygen and liquid propane. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

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PRESERVATION THROUGH UNIVERSAL PREPARATION OF FRESH-FROZEN TISSUE SAMPLES FOR MULTIMODAL IMAGING WORKFLOWS Article 28 May 2024 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * National Heart

Institute, Bethesda, 14, Maryland JOHN L. STEPHENSON Authors * JOHN L. STEPHENSON View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS

Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE STEPHENSON, J. Caution in the Use of Liquid Propane for freezing Biological Specimens. _Nature_ 174, 235 (1954).

https://doi.org/10.1038/174235b0 Download citation * Issue Date: 31 July 1954 * DOI: https://doi.org/10.1038/174235b0 SHARE THIS ARTICLE Anyone you share the following link with will be able

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