New leukemia-killing compounds discovered

NEW STUDY HIGHLIGHTS POTENTIAL OF MITOCHONDRIA-TARGETED CHEMOTHERAPIES Scientists have discovered promising new drugs that work in concert with other drugs to deliver a deadly one-two punch

to leukemia. The researchers are from Rice University and the University of Texas MD Anderson Cancer Center. Although the potential drugs are still years away from being tested in cancer

patients, a study published recently in the journal _Leukemia_ underscores their potential and the innovative methods that led to their discovery. Previous studies by the research teams of

Rice biochemist Natasha Kirienko and MD Anderson physician-scientist Marina Konopleva screened around 45,000 small-molecule compounds to find a few that targeted mitochondria. In the new

research, they chose eight of the most promising compounds and identified between five and 30 closely related analogs for each. Then they conducted tens of thousands of tests to

systematically determine how toxic each analog was to leukemia cells, both when administered individually or in combination with existing chemotherapy drugs like doxorubicin. “One of the big

challenges was to establish optimal conditions and doses for testing on both cancer cells and healthy cells,” said study lead author Svetlana Panina, a researcher at the University of Texas

at Austin who conducted the research during her postdoctoral studies at Rice. “The results from our previously published cytotoxicity assay were helpful, but very little is known about

these small-molecule compounds. None of them had been thoroughly described in other studies, and we had to essentially start from scratch to determine how much to use, what they do in cells,

everything. All the doses and treatment conditions had to be adjusted by multiple preliminary experiments.” MITOPHAGY AS A KEY MECHANISM AGAINST LEUKEMIA Kirienko’s lab had shown in prior

work that the eight compounds targeted energy-producing machinery inside cells called mitochondria. Dozens to thousands of mitochondria are at work every minute in every living cell, and

like all machines, they wear out with use. The eight compounds induce mitophagy, the housekeeping routine cells use to decommission and recycle mitochondria that are past their prime. Cells

can temporarily forgo mitophagy to get an emergency energy boost during times of extreme stress. In fact, cancer is notorious for hijacking these sorts of programs to fuel pathological

growth. Previous research, for instance, has shown leukemia cells have far more damaged mitochondria than healthy cells and are also more sensitive to mitochondrial damage than healthy

cells. Kirienko and Konopleva hypothesized that mitophagy-inducing drugs might weaken leukemia cells and make them more susceptible to chemotherapy. “We hypothesized that if they activate

mitophagy, they may be particularly toxic to leukemia cells,” said Kirienko, the corresponding author of the new study. “And indeed, we found that six of the eight small-molecule compounds

were deadly to leukemia cells. We then wanted to study them more in depth. So we looked at closely related molecules, and we looked at combinations.” SYNERGY BETWEEN NEW COMPOUNDS AND

CHEMOTHERAPY When two or more drugs are given in combination, scientists can also administer them individually and compare the effectiveness of each regimen. “There is a number called

synergy coefficient that quantifies interactions between drugs,” Kirienko said. “If the coefficient is negative, the drugs are antagonistic and work against one another. Zero means no

effect, and positive numbers indicate positive interactions. Anything above 10 is considered synergistic.” For example, one currently prescribed drug combination for leukemia — doxorubicin

and cytarabine — has a synergy coefficient of 13, Kirienko said. The team’s experiments showed several mitophagy-inducing compounds were significantly more synergistic with doxorubicin. The

most synergistic, a compound called PS127B, had a coefficient of 29. “The point of synergy is that there are concentrations, or dosages, where a single drug doesn’t kill,” Kirienko said.

“There is no death of healthy cells or cancer cells. But administering those same concentrations in combination can kill a considerable amount of cancer cells and still not affect healthy

cells.” The research team began by testing the toxicity of its mitophagy-inducing compounds and combinations against acute myeloid leukemia (AML) cells, the most commonly diagnosed form of

the disease. Then they tested the six most effective AML-killing compounds against other forms of leukemia and found five were also effective at killing acute lymphoblastic leukemia (ALL)

cells and chronic myelogenous leukemia (CML) cells. Control studies found all the mitophagy-inducing drugs caused far less harm to healthy cells. PROMISING RESULTS FROM PDX MODELS IN MICE In

their final experiments, the scientists tested one of the most effective mitochondria-targeting compounds, PS127E, using a cutting-edge technique called a patient-derived xenograft (PDX)

model. In PDX, also referred to as a “mouse clinical trial,” mice are implanted with cancer cells from a leukemia patient. Once the cells grow, the mouse is exposed to a drug or combination

of drugs as a closer-than-cells test of the treatment’s effect. PDX tests on one compound, PS127E, showed it was effective at killing AML cells in mice. “Although this is very promising,

we’re still some distance from having a new treatment we can use in the clinic,” Kirienko said. “We still have a lot to discover. For example, we need to better understand how the drugs work

in cells. We need to refine the dose we think would be best, and perhaps most importantly, we need to test on a wide variety of AML cancers. AML has a lot of variations, and we need to know

which patients are most likely to benefit from this treatment and which are not. Only after we’ve done that work, which may take a few years, would we be able to start testing in humans.”

Reference: “Novel mitochondria-targeting compounds selectively kill human leukemia cells” by Svetlana B. Panina, Jingqi Pei, Natalia Baran, Elissa Tjahjono, Shraddha Patel, Gheath Alatrash,

Sergej Konoplev, Leonid A. Stolbov, Vladimir V. Poroikov, Marina Konopleva and Natalia V. Kirienko, 7 June 2022, _Leukemia_. DOI: 10.1038/s41375-022-01614-0 Additional study co-authors

include Jingqi Pei and Elissa Tjahjono of Rice, Natalia Baran, Shraddha Patel, Gheath Alatrash and Sergej Konoplev of MD Anderson, and Leonid Stolbov and Vladimir Poroikov of the Institute

of Biomedical Chemistry in Moscow. The research was supported by the Cancer Prevention and Research Institute of Texas (RR150044), the National Institutes of Health (R35GM129294,

R01CA231364, P50CA100632) and the Russian Federation Fundamental Research Program (1220301001705). NEVER MISS A BREAKTHROUGH: JOIN THE SCITECHDAILY NEWSLETTER.