High strength and low swelling composite hydrogels from gelatin and delignified wood

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High strength and low swelling composite hydrogels from gelatin and delignified wood"


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ABSTRACT A delignified wood template with hydrophilic characteristics and high porosity was obtained by removal of lignin. Gelatin was infiltrated into the delignified wood and further


crosslinked with a natural crosslinker genipin to form hydrogels. The composite hydrogels showed high mechanical strength under compression and low swelling in physiological condition. The


effect of genipin concentrations (1, 50 and 100 mM) on structure and properties of the composite hydrogels were studied. A porous honeycomb structure with tunable pore size and porosity was


observed in the freeze-dried composite hydrogels. High elastic modulus of 11.82 ± 1.51 MPa and high compressive yield stress of 689.3 ± 34.9 kPa were achieved for the composite hydrogel with


a water content as high as 81%. The equilibrium water uptake of the freeze-dried hydrogel in phosphate buffered saline at 37 °C was as low as 407.5%. These enables the delignified wood


structure an excellent template in composite hydrogel preparation by using infiltration and in-situ synthesis, particularly when high mechanical strength and stiffness are desired. SIMILAR


CONTENT BEING VIEWED BY OTHERS SIMULTANEOUS/DIRECT CHEMOMECHANICAL DENSIFICATION AND DOWNSIZING OF WEAK PAULOWNIA WOOD TO PRODUCE A STRONG, UNIDIRECTIONAL, ALL-WOODEN NANOCOMPOSITE Article


02 March 2023 WOOD XEROGEL FOR FABRICATION OF HIGH-PERFORMANCE TRANSPARENT WOOD Article Open access 17 May 2023 FACILE FABRICATION OF A NOVEL SELF-HEALING AND FLAME-RETARDANT HYDROGEL/MXENE


COATING FOR WOOD Article Open access 01 February 2023 INTRODUCTION Recently, utilizing the inherent nanocellulose fibril orientation, anisotropic tissue organization and cellular structure


in wood, synthetic polymers and inorganic materials have been introduced into delignified wood to generate composites with transparency, electrical conductivity, and novel functions for


applications in load-bearing materials, environmental remediation, actuators, solar steam generation, etc.1,2,3,4. Wood-based hydrogels have also been synthesized by filling the micro


channels of delignified wood with the polyacrylamide (PAM) hydrogel precursor followed by free-radical polymerization5. The wood/PAM hydrogel demonstrated remarkably enhanced fracture


tensile strength and modulus as compared to the PAM hydrogel owing to the skeletons of natural aligned cellulose nanofibers (CNFs) and the strong interfacial hydrogen bonding in the


hydrogel. This top-down approach opened up a new way to synthesize strong hydrogels exploiting the advantages of the cellular structure with highly aligned CNFs bundles in the cell walls in


delignified wood, which provides not only mechanical support but also liquid conduction through the natural aligned micro and nano channels. Gelatin is a mixture of peptide hydrolysed from


collagen and has been widely used in food, pharmaceutical and cosmetic industries as a stabilizing ingredient since it is non-toxic, biocompatible, and biodegradable. In tissue engineering


and biomedicine, gelatin-based hydrogels with porous network structures can provide an ideal environment for cells attachment and promote their colonization, migration and


proliferation6,7,8. The gelation of mammalian and warm-water fish gelatin in aqueous solution occurs at temperature below 25 °C due to physical folding of the polypeptide backbone, i.e. the


single-strand to triple-helix transition of gelatin chains. The gelling temperature of cold-water fish gelatin is even lower, at around 10 °C9,10. This gelation process is reversible as the


temperature increases to 37 °C. To avoid the dissolution of gelatin at the physiological temperatures, chemical or enzymatic crosslinking is usually employed11,12,13. Conventional synthetic


crosslinking reagents such as glutaraldehyde, diisocyanates, and carbodiimides, as well as genipin, a natural crosslinker with low acute toxicity, have been used for gelatin crosslinking.


However, the poor mechanical performance and high swelling ratio of gelatin largely limit the application of gelatin hydrogels. The strategies to substantially improve mechanical properties


of hydrogel include hybridization with nanomaterials or incorporation of multiple components with different crosslinking mechanisms involving chemical bonding and physical entanglement14.


Various matrixes, either organic or inorganic, have been hybridized with gelatin to improve its mechanical and thermal performance15,16,17,18,19. For example, crosslinked porous


gelatin/chitosan scaffolds with controllable mechanical properties for load bearing soft tissues have been prepared using genipin20. Formation of interpenetrating network (IPN) and semi-IPN


structures in gelatin/chitosan hydrogels could also enhance the mechanical properties. Gelatin was modified with methacrylic anhydride and covalently crosslinked by ultraviolet light


radiation, while chitosan was crosslinked by hydrophobic interactions at high pH19. Gelatin/alginate hydrogels with IPN structures by chemical crosslinking of gelatin with genipin or


carbodiimide together with ionic crosslinking of alginate with calcium or zinc ions have also been prepared. The hydrogel was further reinforced with cellulose nanocrystals (CNCs) and


demonstrated good mechanical properties comparable to natural cartilage21,22. Dialdehyde CNCs prepared by sodium periodate oxidation was able to crosslink gelatin to form hydrogel with


improved mechanical properties and thermal stability23. CNFs have also been dispersed into crosslinked gelatin/chitosan matrix to fabricate 3D porous scaffolds by freeze-drying24. The


scaffolds demonstrated high porosity and viscoelasticity when immersed in phosphate buffered saline (PBS). Bacterial cellulose (BC)/gelatin composite hydrogels crosslinked with


_N_-(3-dimethy-laminopropyl)-_N_′-ethylcarbodiimide hydrochloride have also showed significant enhancement in mechanical strength and stiffness owing to the natural ultrafine-fibre network


structures of BC25,26,27. The fracture stress and elastic modulus of BC/gelatin hydrogel under compression were reported to be 3.7 MPa and 1.7 MPa with water content of 82.8 wt%,


respectively27. The interfacial interaction and bonding between cellulose and gelatin and the formation of CNF network structure are important to the mechanical properties of gelatin/CNFs


hydrogels. To further increase their mechanical properties while maintaining high water content (> 80 wt%), the formation of oriented structure of aligned CNFs in the hydrogel is highly


desirable. However, this is rather challenging and requires shear-induced alignment when using CNFs by bottom-up assembly approaches28,29. In this work, delignified wood with inherently


aligned CNFs and native porous cell wall structure is used as mechanical reinforcement and structural confinement for gelatin hydrogels, aiming to enhance the compressive mechanical


properties of gelatin hydrogel as well as decreasing its swelling under physiological condition. Composite hydrogels from delignified wood and gelatin have been prepared by infiltration of


gelatin followed by chemical crosslinking with genipin at different concentrations and characterized in terms of structural features (porosity and pore size), molecular configuration,


mechanical properties and swelling behaviour. As illustrated in Fig. 1, lignin was removed from wood structure and the cellular structure with inherent CNFs alignment in the cell wall was


preserved. Gelatin was infiltrated in the channels of the delignified wood and crosslinked by genipin. The microstructure of the delignified wood/gelatin composite hydrogels was


characterized by optical microscopy and field emission scanning electron microscopy (FE-SEM). The compressive mechanical properties and the swelling behaviour of the composite hydrogels were


studied. RESULTS AND DISCUSSION STRUCTURE OF THE COMPOSITE HYDROGEL Balsa wood was delignified by using 1 wt% sodium chlorite at 80 °C. The main functional substance in sodium chlorite


delignification is chlorine dioxide, which has been widely used for disinfection, sterilization in food and medical application owing to its low toxicity. The delignified wood was washed


intensively with deionized water before the infiltration of gelatin. Delignified wood showed a white colour (Fig. 2a) as most of lignin and chromophores have been removed from the wood


structure. This was further confirmed by the disappearing of lignin-attributed peaks30 at 1596 cm−1 (symmetric ring stretching), 1504 cm−1 (asymmetric ring stretching) and 1464 cm−1


(asymmetric C–H deformation) in the ATR-FTIR spectrum of delignified wood as compared to the original Balsa wood (Fig. 3). Besides, hemicelluloses remained in the delignified wood as


indicated by the peak at 1730 cm−1 corresponding to the C=O stretching frequency of carbonyl groups in hemicellulose. The crosslinking strategies significantly impact the structure and


properties of gelatin-based hydrogel31,32. The complete gelation of gelatin (37.5 wt%) was achieved within 35 min at 37 °C when 1 wt% (44 mM) genipin was used as a crosslinker33. However,


the complete infiltration of gelatin into porous delignified wood structure takes much longer time. To avoid gelling, gelatin from bovine skin was first infiltrated into the delignified wood


at 50 °C for one week. The composites were then kept overnight at 4 °C, which is below the upper critical solution temperature of gelatin, to allow physical gelation of gelatin.


Subsequently, the composite hydrogels were further chemically crosslinked in a genipin solution for 48 h, to assure complete crosslinking of infiltrated gelatin in the composite. The initial


genipin concentration and the compositions of gelatin, delignified wood, and water in the resulting composite hydrogels are summarized in Table 1. The delignified wood/gelatin composite


hydrogels with water content of around 81 wt% were successfully prepared. The water content was lower than those for neat gelatin hydrogel due to the interaction between gelatin and


delignified wood and the structural confinement provided by the wood cell wall structure as evidenced by FTIR analysis and swelling behaviour discussed in latter sections. Nonetheless, a


highly hydrated network (water content > 80 wt%) was maintained in the composite hydrogels, which is of great interests for ultra-stiff/strong hydrogel preparation34. Both gelatin


hydrogel and the delignified wood/gelatin hydrogels showed a dark blue colour (Fig. 2b,c) indicating the successful chemical crosslinking of gelatin by genipin. This characteristic colour is


typically observed for dyeing in food, textile, and tattoo when the amine groups are crosslinked with genipin. Infiltration of gelatin into the lumen of the wood structure was confirmed by


the microscopy observation (Fig. 2d,e). This was further confirmed by FTIR analysis (Fig. 3). The absorption band at 1527 cm-1 in neat gelatin was ascribed to NH bending (amide II). The


shift of the amide II band from 1527 to 1540 cm-1 (Fig. 3b) was observed in delignified wood/gelatin hydrogels, indicating that the presence of delignified wood prevented the formation of


β-sheet structure, resulting in more random coil to α-helix structure during the physical folding of gelatin in cooling process35. The peak at 897 cm-1 (Fig. 3a) is originated from the CH


deformation at C1 position associated with C–O–C stretching of the β-glycosidic linkages in cellulose and hemicellulose36. The intensity of the shoulder peak at 897 cm-1 in the composite


hydrogels decreased with increasing crosslinking density at higher genipin concentrations, which indicating an increased hydrogen bonding between gelatin and delignified wood. Indeed, the


broadening of the OH stretching vibration region centred at 3340 cm-1 (Fig. 3b) and the NH stretching of hydrogen-bonded amide group at 3300 cm-1 was observed for the composite hydrogel with


increasing genipin concentration37. The peaks at 3080 and 2940 cm-1 (Fig. 3a) were attributed to the CH3 stretching and CH2 stretching of amide B structure, respectively38. The relative


intensity between 3080 to 2940 cm-1 (_I__3080_/_I__2940_) of genipin crosslinked delignified wood/gelatin composite was higher than the neat gelatin, indicating stronger entanglement in the


gelatin network. The effect of genipin concentrations on the morphology and pore structure of delignified wood/gelatin hydrogels was further studied by FE-SEM (Fig. 4) using freeze-dried


samples. Their pore size and porosity data are summarized in Table 2. As shown in Fig. 4a, the cross section of delignified balsa wood possessed a honeycomb-like structure which is composed


of hollow fiber cells with diameters between 20 and 50 μm. Nano- and micron-scale pores were observed in both cell walls and cell wall corners (Fig. 4b), and a total porosity of 93.4 ± 0.6%


was obtained. Such porous structure allowed the penetration of gelatin into the cell wall structure and facilitated the interaction of gelatin with cellulose and hemicellulose in delignified


wood. The fixation of gelatin in delignified wood was enabled by chemical crosslinking with genipin. When only 1 mM genipin solution was applied, the composite hydrogel shows similar


macroscopic structure as delignified wood (Fig. 4c). Due to the low initial concentration of genipin, network structure of gelatin did not form in the lumens. Gelatin was mainly adsorbed


onto the surface of lumens and infiltrated into the cell wall and cell wall corners, resulting in a decreased porosity of 83.7 ± 0.2%. Indeed, smooth surface was observed at both cell wall


and cell wall corners (Fig. 4d). When the genipin concentration was increased to 50 mM, gelatin network was formed within lumens and showed a pore size of 6.0 ± 1.2 μm (Fig. 4e,f). As the


concentration of genipin was increased to 100 mM, denser gelatin network was clearly observed in lumens (Fig. 4g,h), and the crosslinked gelatin aerogel exhibits a porous structure with


relatively smaller pore size of 2.3 ± 0.5 μm. Consequently, the total porosity of delignified wood/gelatin hydrogel decreased to 72.7 ± 0.3% and 69.0 ± 0.2% when 50 and 100 mM genipin were


applied, respectively. Beyond short-range crosslinking between amino acids, genipin also undergoes a long-range intermolecular crosslinking which involves self-crosslinking between genipin


monomers. By tuning the degree of polymerization of polygenipin, the pore size of gelatin scaffold certainly can be controlled for different purposes39. COMPRESSIVE MECHANICAL PROPERTIES The


typical compressive stress–strain curves of gelatin, delignified wood, and delignified wood/gelatin hydrogels are shown in Fig. 5 and their mechanical property data are summarized in Table


3. When neat gelatin was chemically crosslinked with genipin, the enhancement in elastic modulus, fracture stress and fracture strain was achieved owing to higher crosslinking density with


increasing concentration of genipin (Table 3). The elastic modulus of gelatin hydrogel increased from 22.5 ± 2.4 kPa to 135.8 ± 23.0 kPa as the genipin concentration increased from 1 to 100 


mM, respectively. The crosslinked gelatin hydrogel showed elastic behaviour and large deformation (compressive strain up to 50%) was induced with low stress load (Fig. 5a). Increasing


crosslinking density resulted in denser polymer network with decreased water content (from 88.2 ± 0.1 to 85.9 ± 0.1 wt%, Table 1), which substantially enhanced the mechanical properties of


neat gelatin hydrogel27. As the genipin concentration increased from 1 to 100 mM, significant increase in fracture strain and fracture stress for neat gelatin hydrogel was observed (Table


3). The impact of crosslinking density on the mechanical properties of gelatin is significant due to its amorphous nature and lack of rigidity27. Relatively high fracture stress (1.01 ± 0.37


 MPa) was achieved at a fracture strain of 75.3 ± 4.9% by using 100 mM genipin. By contrast, the delignified wood hydrogel showed a completely different compressive stress–strain curve with


an initial linear elastic deformation followed by a non-linear plastic yielding and the highest stress (311.0 ± 21.6 kPa) was reached at a compressive strain of 3.2 ± 0.3% (Fig. 5b). Due to


the misalignment of fibres, shear force was generated along with compression, thus tangential displacement of fibres under stress transitioned into a plateau when the strain was between 5


and 90%. Benefit from the crystalline nature of cellulose and oriented organization of cellulose microfibrils along axial direction in the secondary cell wall of wood40, high stiffness was


achieved with an elastic modulus of 21.89 ± 3.76 MPa for the delignified wood hydrogel. When gelatin was infiltrated into delignified wood and crosslinked by genipin, the composite hydrogels


showed similar compressive stress–strain profile as delignified wood, and a decrease in elastic modulus with an enhancement in yield stress and an increase in yield strain were observed


(Table 3). Although the composite hydrogels had much lower water content (higher solid density) as compared to delignified wood (Table1), the elastic modulus of the composite hydrogels


decreased with the increasing genipin concentration as compared to delignified wood. This suggests that the volume fraction of infiltrated gelatin inside the wood cell walls and cell wall


corners were increased with increasing crosslinking of gelatin. Indeed, the interaction between gelatin and delignified wood was also enhanced with increasing genipin concentration as


confirmed by FTIR analysis. When 1 mM genipin was applied, slight increase in both yield stress and yield strain was found for composite hydrogel as compared to delignified wood. As the


concentrations of genipin were increased to 50 and 100 mM, the yield stress of delignified wood/gelatin hydrogel increased two-fold compared to delignified wood to 652.6 ± 49.8 kPa and 689.3


 ± 34.9 kPa, respectively, owing to the dense crosslinking between amino acid chains of gelatin and subsequent formation of hydrogel network within the cell lumens of delignified wood as


revealed in Fig. 4e–h. The network structure inside wood cell lumens contributed to energy dissipation during compression, improving the resistance of composite hydrogel to yield, hence the


yield strain was increased to 9.7 ± 0.2% and 10.2 ± 0.6%, respectively. The fracture strains of delignified wood and the composite hydrogels are all higher than 95%. The compressive stress


of the composite hydrogels at 95% compressive strain was increased significantly with increasing genipin concentration as compared to that for delignified wood. With 100 mM genipin, the


compressive stress at 95% compressive strain was as high as 14.44 ± 2.65 MPa, one to two magnitude higher than that for neat delignified wood and the fracture stress for neat gelatin


crosslinked with 100 mM genipin (Table 3). The elastic modulus of the composite hydrogel (11.82 ± 1.51 MPa) was also two magnitude higher than that for neat gelatin hydrogel at 100 mM


genipin. Such synergistic enhancement in both elastic modulus and compressive stress indicated the mutual toughening effect owing to the formation of porous structure of gelatin in the


lumens of honeycomb like wood cell structure and strong intermolecular hydrogen bonding between infiltrated gelatin and delignified wood inside the wood cell walls and cell wall corners.


SWELLING BEHAVIOUR The swelling behaviour is another key factor which is strongly influenced by the structure of composite hydrogel. To study the swelling behaviour of neat gelatin hydrogel,


delignified wood and composite hydrogels, samples were freeze-dried and then rehydrated in 0.1 M PBS (pH 7.4) buffer at 37 °C for 48 h to reach an equilibrium state. As shown in Fig. 6, the


equilibrium water uptake of delignified wood was 1747.5 ± 14.1% because of its high porosity and hydrophilicity. Freeze-fried neat gelatin hydrogel also showed high water uptake, around


2120.1 ± 153.9%, when 1 mM genipin was applied. The loosely crosslinked gelatin network enabled accommodating large volume of phosphate buffer. The swelling of neat gelatin hydrogel was


restricted when higher concentration of genipin was applied. A water uptake of 825.1 ± 17.7% was obtained at a genipin concentration of 100 mM. A plausible explanation is the formation of a


rigid network through genipin crosslinking, which reduced the accessible hydrophilic groups as well as the extensibility of gelatin network. Different from neat gelatin, delignified


wood/gelatin hydrogels showed less dependence on the genipin concentration. The water uptake values of the composite hydrogels were 431.1 ± 5.7%, 427.9 ± 4.4%, and 407.5 ± 5.2% at a genipin


concentration of 1, 50, and 100 mM, respectively. Such lower water uptake values as compared to delignified wood and neat gelatin were resulted from the infiltration of gelatin into wood


cell wall and the formation of strong intermolecular hydrogen bonding between delignified wood and gelatin after genipin crosslinking. These values also suggested that the corresponding


water content of the PBS rehydrated composite hydrogels were ca. 81.2%, 81.1%, and 80.3%, respectively, same to the original water content in the composite hydrogels (Table 1). This


indicates that unlike the neat gelatin hydrogel, further swelling of the composite hydrogel did not occur. Indeed, the accessibility of delignified wood cell wall and hydrophilicity of both


components were significantly constrained. Particularly, the rigid nature of the cell wall structure in delignified wood restricted the swelling of gelatin. As a comparison, when 30 wt%


gelatin was crosslinked with _N_-(3-dimethy-laminopropyl)-_N_′-ethylcarbodiimide hydrochloride (EDC) within the bacterial cellulose (BC) scaffold27, the double-network (DN) composite


hydrogel showed a compressive stress of 3.7 MPa, a modulus of 1.7 MPa, and an a degree of swelling of 5.8, which is equal to a water uptake value of 480%. Another example is TEMPO-oxidized


CNF/gelatin (1:10 by weight) composite, in which gelatin was crosslinked initially through physical dehydrothermal treatment and then chemically with 6 wt% hexamethylenediamine and/or 1 wt%


genipin. The resulted composite hydrogel showed a compressive stress of 25.6 kPa, an elastic modulus of 35.2 ± 3.1 kPa and a swelling ratio of 10.8 ± 0.6, which is equal to a water uptake


value of around 1080%8. Photo-crosslinked IPN hydrogel consisting of chitosan and gelatin methacryloyl achieved a compressive modulus of 116.08 ± 9.62 kPa and an equilibrium water uptake


value of around 1000%19. Taking advantage of the native aligned cellulose fibrils in wood cell wall and honeycomb like cellular structure of wood5, the delignified wood/gelatin hydrogel


prepared in this work was proved to be both mechanically tough under compression (Fig. 5) and highly resistant to swelling under physiological condition (Fig. 6). Besides, the water content


of delignified wood/gelatin hydrogel (ca.81%, Table 1) is among the highest which is close to that in natural cartilage21. CONCLUSIONS A bio-based composite hydrogel composed of gelatin and


delignified wood was successfully prepared through infiltration of gelatin into the porous wood template at 50 °C and subsequent crosslinking with genipin at 4 °C. The composite hydrogels


demonstrated tunable porous structure with high mechanical strength and high resistance to swelling during rehydration in phosphate buffer (0.1 M, pH 7.4) at 37 °C. With increasing genipin


concentration from 1 to 100 mM, the composite hydrogels showed an increase in compressive yield stress as high as 689.3 ± 34.9 kPa owing to the infiltration of gelatin into the cell wall and


cell wall corner and the formation of gelatin network within lumen of the well-preserved wood cell structure in delignified wood. The elastic modulus of the composite hydrogel was 11.82 ± 


1.51 MPa at a genipin concentration of 100 mM, two magnitude higher than the corresponding neat gelatin hydrogel. The swelling of gelatin was confined within lumen of the wood cell,


resulting in the water uptake at equilibrium state as low as 407.5 ± 5.2%. These unique properties are superior to those for the DN hydrogel of BC/gelatin and IPN hydrogels of CNF/gelatin


and chitosan/gelatin. In addition, both pore size of the infiltrated gelatin and porosity of the freeze-dried composite hydrogels were tunable by varying the genipin concentration. This work


demonstrated wood structure an excellent template in preparation of high-performance composite hydrogels, taking advantages of inherently aligned CNFs and native porous cell wall structure


for mechanical reinforcement and structural confinement. METHODS REAGENTS AND MATERIALS Gelatin from bovine skin (75 Bloom), sodium chlorite, sodium acetate, acetic acid, glycine, sodium


dihydrogen phosphate dihydrate and sodium phosphate dibasic dihydrate were purchased from Sigma-Aldrich and used as received. Genipin powder (98%, HPLC) was a product of Zhixin


Biotechnology, China. Wood blocks (10 × 10 × 10 mm3, _Longitudinal_ × _Radial_ × _Tangential_) were cut from air-dried balsa wood (_Ochroma pyramidale_) sticks with a density of 175 kg/m3


purchased from Wentzel’s Co. Ltd., Sweden. PREPARATION OF DELIGNIFIED WOOD Balsa wood blocks were immersed in 1 wt% sodium chlorite buffered with acetate solution (PH 4.6) overnight at room


temperature prior to heating up. The delignification was then carried out at 80 °C for 24 h. After washing thoroughly with deionized water, the delignified wood blocks were kept in water


until further use. PREPARATION OF DELIGNIFIED WOOD/GELATIN COMPOSITE HYDROGELS Gelatin was dissolved in deionized water at concentration of 30 wt% and stored overnight at 50 °C. The


delignified wood blocks were then transferred into the gelatin solution and kept for 1 week at 50 °C to ensure a complete infiltration of gelatin into the delignified wood.


Gelatin-impregnated wood samples were then taken out and cooled down to 4 °C overnight to allow physical crosslinking of gelatin. The samples were then crosslinked in a genipin solution with


different concentrations: 1, 50, and 100 mM at 4 °C for 48 h. The composite hydrogels were then washed with 1 wt% glycine solution followed by deionized water to remove the unreacted


genipin. Neat gelatin hydrogel was prepared in the same way as the composite hydrogel. ATTENUATED TOTAL REFLECTION FOURIER TRANSFORM INFRARED SPECTROSCOPY (ATR-FTIR) ATR-FTIR spectra of


original wood, delignified wood, delignified wood/gelatin hydrogel, and neat gelatin without genipin crosslinking were obtained with a FTIR spectrometer (Spectrum 2000, Perkin-Elmer, USA).


The resolution was 4 cm-1 and 32 scans were accumulated over a range of 600–4000 cm-1 for each sample. Prior to FTIR characterisation, thin slices of each samples were obtained with


microtome and freeze-dried. OPTICAL MICROSCOPY AND FE-SEM The morphology of crosslinked gelatin within the delignified wood structure was characterized by using an optical microscope (LSM


510 Pa, Zeiss, Germany) in wet state. The microstructure was further characterized by using a field emission scanning electron microscope (FE-SEM, S-4800, Hitachi, Japan) working at low


acceleration voltage (3 kV) and short working distance (8 mm). Thin Sections (200 μm in thickness) were obtained from the cross section of the delignified wood/gelatin hydrogel and


delignified wood with a sliding microtome (SM 2010R, Leica, Germany). The hydrogel sections were then frozen at − 20 °C for 24 h and lyophilized with a freeze-dryer for 48 h. Freeze-dried


sections were fixed onto metal stubs with carbon tape and then sputtered with platinum-palladium with a thickness of 3 nm. Pore size of the infiltrated gelatin in the freeze-dried composite


hydrogel was measured from the FE-SEM images using Image-J software and 100 pores were measured for each sample41,42. COMPRESSION TEST Compression test was carried out on a universal


mechanical tester (Instron-5566, Instron, USA) equipped with a 10 kN loading cell. The strain rate was 1 mm/min. Both gelatin hydrogel and delignified wood/gelatin hydrogels were taken out


from water and tested immediately after wiping off the excessive water on the surface. Load was applied to the composite hydrogel samples along the longitudinal direction of the wood. At


least five specimens were tested for each sample. SWELLING BEHAVIOUR Delignified wood, delignified wood/gelatin hydrogels and neat gelatin hydrogels were freeze-dried and then rehydrated in


phosphate buffer (0.1 M, pH 7.4) at 37 °C for 48 h. The equilibrium water uptake (_Q_) was calculated using the following equation: $${\text{Q }} = {\text{ }}\frac{{W_{e} - W_{d} }}{{W_{d}


}} \times 100\%$$ (1) where _W__e_ is the weight of the sample at equilibrium condition and _W__d_ is the weight of the dry sample. POROSITY The porosity of freeze-dried composite hydrogels


was estimated based on their bulk density (_ρ__b_) calculated from dry weight and apparent volume (geometry), and true density (_ρ__t_) measured with a helium pycnometer (AccuPyc 1330,


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data. _BMC Bioinform._ 18, 529. https://doi.org/10.1186/s12859-017-1934-z (2017). Article  Google Scholar  Download references ACKNOWLEDGEMENTS The authors thank Wallenberg Wood Science


Center (WWSC) and the Swedish Foundation for International Cooperation in Research and Higher Education STINT (CH2017-7275) for supporting this work. S. Wang thanks the China Scholarship


Council (CSC) for supporting his PhD study. FUNDING Open Access funding provided by Kungliga Tekniska Hogskolan. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Glycoscience,


Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Centre, 106 91, Stockholm, Sweden


Shennan Wang, Kai Li & Qi Zhou * Wallenberg Wood Science Center, Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, 100 44, Stockholm, Sweden Shennan Wang, 


Kai Li & Qi Zhou Authors * Shennan Wang View author publications You can also search for this author inPubMed Google Scholar * Kai Li View author publications You can also search for


this author inPubMed Google Scholar * Qi Zhou View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS S.W., K.L. and Q.Z. developed the concepts.


S.W. performed the experiments and analyzed the data. S.W. and Q.Z. wrote the main manuscript. All authors reviewed the manuscript. CORRESPONDING AUTHOR Correspondence to Qi Zhou. ETHICS


DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. ADDITIONAL INFORMATION PUBLISHER'S NOTE Springer Nature remains neutral with regard to jurisdictional claims


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http://creativecommons.org/licenses/by/4.0/. Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Wang, S., Li, K. & Zhou, Q. High strength and low swelling composite hydrogels


from gelatin and delignified wood. _Sci Rep_ 10, 17842 (2020). https://doi.org/10.1038/s41598-020-74860-w Download citation * Received: 02 March 2020 * Accepted: 07 October 2020 * Published:


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