Membrane association of monotopic phosphoglycosyl transferase underpins function

ABSTRACT Polyprenol phosphate phosphoglycosyl transferases (PGTs) catalyze the first membrane-committed step in assembly of essential glycoconjugates. Currently there is no

structure–function information to describe how monotopic PGTs coordinate the reaction between membrane-embedded and soluble substrates. We describe the structure and mode of membrane

association of PglC, a PGT from _Campylobacter concisus_. The structure reveals a unique architecture, provides mechanistic insight and identifies ligand-binding determinants for PglC and

the monotopic PGT superfamily. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your

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SIMILAR CONTENT BEING VIEWED BY OTHERS PEPTIDOGLYCAN BIOSYNTHESIS IS DRIVEN BY LIPID TRANSFER ALONG ENZYME-SUBSTRATE AFFINITY GRADIENTS Article Open access 27 April 2022 MOLECULAR BASIS OF

THE INOSITOL DEACYLASE PGAP1 INVOLVED IN QUALITY CONTROL OF GPI-AP BIOGENESIS Article Open access 02 January 2024 INSIGHT INTO THE DUAL FUNCTION OF LIPID PHOSPHATE PHOSPHATASE PGPB INVOLVED

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Natl Acad. Sci. USA_ 98, 10037–10041 (2001). Article  CAS  PubMed  PubMed Central  Google Scholar  Download references ACKNOWLEDGEMENTS We thank K. Rajashankar for assistance with phasing

and the staff at NECAT (APS) for facilitating X-ray data collection. Financial support for this work was provided by the National Institutes of Health: R01-GM039334 to B.I., the Predoctoral

Training Program in the Biological Sciences (T32-GM007287) to S.E. and the Biomolecular Pharmacology Program Grant (T32-GM008541) to L.C.R. This work is also based upon research conducted at

the Northeastern Collaborative Access Team beamlines 24-ID-E and 24-ID-C, which is funded by the National Institute of General Medical Sciences from the National Institutes of Health (P41

GM103403). AUTHOR INFORMATION Author notes * These authors contributed equally: Leah C. Ray and Debasis Das. AUTHORS AND AFFILIATIONS * Program in Biomolecular Pharmacology, Boston

University School of Medicine, Boston, MA, USA Leah C. Ray & Karen N. Allen * Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA Debasis Das, Sonya Entova 

& Barbara Imperiali * Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA Debasis Das, Vinita Lukose & Barbara Imperiali * Department of Chemistry,

Boston University, Boston, MA, USA Andrew J. Lynch & Karen N. Allen Authors * Leah C. Ray View author publications You can also search for this author inPubMed Google Scholar * Debasis

Das View author publications You can also search for this author inPubMed Google Scholar * Sonya Entova View author publications You can also search for this author inPubMed Google Scholar *

Vinita Lukose View author publications You can also search for this author inPubMed Google Scholar * Andrew J. Lynch View author publications You can also search for this author inPubMed 

Google Scholar * Barbara Imperiali View author publications You can also search for this author inPubMed Google Scholar * Karen N. Allen View author publications You can also search for this

author inPubMed Google Scholar CONTRIBUTIONS L.C.R. crystallized, collected data, determined the structure, refined and analyzed the model of PglC and performed phosphate release kinetics;

D.D. optimized expression, designed and made constructs, expressed and purified PglC, carried out lipid analysis and analyzed the structure. S.E. designed and performed SCAM analyses. V.L.

designed and purified original constructs for crystallization and A.J.L. obtained the original crystallization conditions. L.C.R., D.D. and S.E. wrote the manuscript. B.I. and K.N.A.

conceived the project, designed experiments, assisted with data analysis and interpretation, and critically edited the manuscript. CORRESPONDING AUTHORS Correspondence to Barbara Imperiali

or Karen N. Allen. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. ADDITIONAL INFORMATION PUBLISHER’S NOTE: Springer Nature remains neutral with regard to

jurisdictional claims in published maps and institutional affiliations. SUPPLEMENTARY INFORMATION SUPPLEMENTARY TEXT AND FIGURES Supplementary Figures 1–11 and Supplementary Tables 1–5

REPORTING SUMMARY DATASET 1 Supplementary Dataset 1 RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Ray, L.C., Das, D., Entova, S. _et al._ Membrane

association of monotopic phosphoglycosyl transferase underpins function. _Nat Chem Biol_ 14, 538–541 (2018). https://doi.org/10.1038/s41589-018-0054-z Download citation * Received: 07

November 2017 * Accepted: 14 March 2018 * Published: 16 May 2018 * Issue Date: June 2018 * DOI: https://doi.org/10.1038/s41589-018-0054-z SHARE THIS ARTICLE Anyone you share the following

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