Surface-enhanced raman scattering holography

ABSTRACT Nanometric probes based on surface-enhanced Raman scattering (SERS) are promising candidates for all-optical environmental, biological and technological sensing applications with

intrinsic quantitative molecular specificity. However, the effectiveness of SERS probes depends on a delicate trade-off between particle size, stability and brightness that has so far

hindered their wide application in SERS imaging methodologies. In this Article, we introduce holographic Raman microscopy, which allows single-shot three-dimensional single-particle

localization. We validate our approach by simultaneously performing Fourier transform Raman spectroscopy of individual SERS nanoparticles and Raman holography, using shearing interferometry

to extract both the phase and the amplitude of wide-field Raman images and ultimately localize and track single SERS nanoparticles inside living cells in three dimensions. Our results

represent a step towards multiplexed single-shot three-dimensional concentration mapping in many different scenarios, including live cell and tissue interrogation and complex

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SIMILAR CONTENT BEING VIEWED BY OTHERS INTERFEROMETRIC SCATTERING MICROSCOPY Article 10 April 2025 DETECTION OF SURFACE ENHANCED RAMAN SCATTERING ACTIVE HOTSPOT USING NEAR FIELD SCANNING

OPTICAL MICROSCOPY Article Open access 08 May 2024 SINGLE-SHOT ISOTROPIC DIFFERENTIAL INTERFERENCE CONTRAST MICROSCOPY Article Open access 12 April 2023 DATA AVAILABILITY The materials and

data that support the findings of this study are available from the corresponding authors on request. CODE AVAILABILITY The software used for data analysis is available from the

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holography. _Opt. Lett._ 42, 4611–4614 (2017). Article  Google Scholar  Download references ACKNOWLEDGEMENTS We thank M. Rivas for her support with the live-cell experiments. We acknowledge

support by the Ministry of Science, Innovation and Universities (MCIU/AEI: RTI2018-099957-J-I00 and PGC2018-096875-B-I00), the Ministry of Economy (MINECO: CTQ2017-88648-R, RYC-2015-19107

and ‘Severo Ochoa’ programme for Centers of Excellence in R&D CEX2019-000910-S), the Catalan AGAUR (2017SGR1369 and 2017SGR883), Fundació Privada Cellex, Fundació Privada Mir-Puig, the

Generalitat de Catalunya through the CERCA programme and the Universitat Rovira i Virgili (FR 2019-B2). N.F.v.H. acknowledges the financial support by the European Commission (ERC Advanced

Grant 670949-LightNet). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * ICFO — Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Barcelona, Spain Matz Liebel 

& Niek F. van Hulst * Department of Physical and Inorganic Chemistry and EMaS, Universitat Rovira i Virgili, Tarragona, Spain Nicolas Pazos-Perez & Ramon A. Alvarez-Puebla * ICREA —

Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain Niek F. van Hulst & Ramon A. Alvarez-Puebla Authors * Matz Liebel View author publications You can also search for

this author inPubMed Google Scholar * Nicolas Pazos-Perez View author publications You can also search for this author inPubMed Google Scholar * Niek F. van Hulst View author publications

You can also search for this author inPubMed Google Scholar * Ramon A. Alvarez-Puebla View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS M.L.

constructed the optical experiment, performed the measurements and analysed the data. M.L. conceived the experiment. N.P.P. and R.A.A.P. synthesized and characterized the nanoparticles. M.L.

wrote the manuscript. M.L., N.F.v.H. and R.A.A.P. contributed to the interpretation of the data, discussion and writing of the manuscript. CORRESPONDING AUTHORS Correspondence to Matz

Liebel, Niek F. van Hulst or Ramon A. Alvarez-Puebla. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. ADDITIONAL INFORMATION PEER REVIEW INFORMATION

_Nature Nanotechnology_ thanks Pasquale Memmolo and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are available. PUBLISHER’S

NOTE Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Supplementary

Figs. 1–8 and Information S1–S7. SUPPLEMENTARY VIDEO 1 Live-cell tracking video supporting Fig. 6, showing bright-field (grey) and SERS (pink) signals. RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Liebel, M., Pazos-Perez, N., van Hulst, N.F. _et al._ Surface-enhanced Raman scattering holography. _Nat. Nanotechnol._ 15, 1005–1011 (2020).

https://doi.org/10.1038/s41565-020-0771-9 Download citation * Received: 20 March 2020 * Accepted: 26 August 2020 * Published: 28 September 2020 * Issue Date: December 2020 * DOI:

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