Utility of cell-free dna from bronchial washing fluid in diagnosis and genomic determination for radiology-suspected pulmonary nodules

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Utility of cell-free dna from bronchial washing fluid in diagnosis and genomic determination for radiology-suspected pulmonary nodules"


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ABSTRACT BACKGROUND Bronchial washing fluid (BWF) is a less-invasive specimen. Due to the limited sensitivity of BWF cellular component diagnosis, the aim of this study was to explore the


potential role of BWF supernatant as a source of liquid biopsy of lung cancer. METHODS This prospective study enrolled 76 suspected and 5 progressed lung cancer patients. Transbronchial


biopsy tissues, BWF supernatant (BWF_Sup) and BWF precipitant (BWF_Pre) were tested by a targeted panel of 1021 genes. RESULTS BWF_Sup cell-free DNA (cfDNA) was superior to tissue biopsy and


BWF_Pre in determining mutational allele frequency, tumour mutational burden, and chromosomal instability. Moreover, BWF_Sup and BWF_Pre achieved comparable efficacy to tissue samples in


differentiating malignant and benign patients, but only BWF_Sup persisted differentiated performance after excluding 55 malignancies pathologically diagnosed by bronchoscopic biopsy. Among


67 malignant patients, 82.1% and 71.6% of tumour-derived mutations (TDMs) were detected in BWF_Sup and BWF_Pre, respectively, and the detectability of TDMs in BWF_Sup was independent of the


cytological examination of BWF. BWF_Sup outperformed BWF_Pre in providing more subclonal information and thus might yield advantage in tracking drug-resistant markers. CONCLUSIONS BWF_Sup


cfDNA is a reliable medium for lung cancer diagnosis and genomic profiles and may provide important information for subsequent therapeutic regimens. Access through your institution Buy or


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PROPOSAL OF A NOVEL PIPELINE INVOLVING PRECISE BRONCHOSCOPY OF DISTAL PERIPHERAL PULMONARY LESIONS FOR GENETIC TESTING Article Open access 17 November 2022 COMPARATIVE ANALYSIS OF EGFR


MUTATIONS IN CIRCULATING TUMOR DNA AND PRIMARY TUMOR TISSUES FROM LUNG CANCER PATIENTS USING BEAM_ING_ PCR Article Open access 08 January 2025 MOLECULAR CHARACTERISTICS OF PRIMARY PULMONARY


LYMPHOEPITHELIOMA-LIKE CARCINOMA BASED ON INTEGRATED GENOMIC ANALYSES Article Open access 08 January 2021 DATA AVAILABILITY The datasets supporting the conclusions of this article are


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Article  CAS  PubMed  PubMed Central  Google Scholar  Download references ACKNOWLEDGEMENTS Prior abstract presentation: Oral presentation, Tumour-derived cell-free DNA from bronchoalveolar


lavage fluid (BALF): a potential liquid biopsy analysis in lung cancer patients; April 3, 2019, American Association for Cancer Research (AACR) Annual Meeting, Atlanta, USA. FUNDING None.


AUTHOR INFORMATION Author notes * These authors contributed equally: Xinyu Zhang, Zhuo Yu, Yaping Xu. AUTHORS AND AFFILIATIONS * Zhongshan Hospital, Fudan University, No. 180 Fenglin Road,


Xuhui District, 200032, Shanghai, China Xinyu Zhang, Yencheng Chao, Qin Hu, Chun Li, Maosong Ye, Yingyong Hou & Xin Zhang * Beijing Tsinghua Changgung Hospital, 168 Litang Road,


Changping District, 102218, Beijing, China Zhuo Yu * Geneplus-Beijing Institute, 9th Floor, No. 6 Building, Peking University Medical Industrial Park, Zhongguancun Life Science Park, 102206,


Beijing, China Yaping Xu, Xiuli Zhu, Liang Cui, Jing Bai, Yuhua Gong, Yanfang Guan & Xuefeng Xia * Ruijin Hospital, Shanghai Jiao Tong University, No. 197 Ruijin Second Road, Huangpu


District, 200025, Shanghai, China Min Zhou * First People’s Hospital, Suzhou University, No. 899 Pinghai Road, Gusu District, 215008, Suzhou, China Jian’an Huang * Zhengzhou Central


Hospital, Zhengzhou University, No. 195 Tongbai Road, Zhongyuan District, 450000, Zhengzhou, China Hua Zhang * Shanghai Sixth People’s Hospital, No 600 Yishan Road, Xuhui District, 200233,


Shanghai, China Tao Ren * First Affiliated Hospital of Zhejiang University, No. 79 Qingchun Road, Shangcheng District, 310002, Hangzhou, China Qian Shen * Fourth Affiliated Hospital of


Zhejiang University, No 88 Jiefang Road, Shangcheng District, 310002, Hangzhou, China Kai Wang * Department of Thoracic Medical Oncology, Hunan Cancer Hospital/the affiliated Cancer Hospital


of Xiangya School of Medicine, Central South University, 283 Tongzipo Road, Yuelu District, 410013, Changsha, Hunan, China Xingxiang Pu * Comprehensive Cancer Center, The Ohio State


University, 460W 12th Ave., Columbus, OH, 43210, USA David P. Carbone Authors * Xinyu Zhang View author publications You can also search for this author inPubMed Google Scholar * Zhuo Yu


View author publications You can also search for this author inPubMed Google Scholar * Yaping Xu View author publications You can also search for this author inPubMed Google Scholar *


Yencheng Chao View author publications You can also search for this author inPubMed Google Scholar * Qin Hu View author publications You can also search for this author inPubMed Google


Scholar * Chun Li View author publications You can also search for this author inPubMed Google Scholar * Maosong Ye View author publications You can also search for this author inPubMed 


Google Scholar * Xiuli Zhu View author publications You can also search for this author inPubMed Google Scholar * Liang Cui View author publications You can also search for this author


inPubMed Google Scholar * Jing Bai View author publications You can also search for this author inPubMed Google Scholar * Yuhua Gong View author publications You can also search for this


author inPubMed Google Scholar * Yanfang Guan View author publications You can also search for this author inPubMed Google Scholar * Min Zhou View author publications You can also search for


this author inPubMed Google Scholar * Jian’an Huang View author publications You can also search for this author inPubMed Google Scholar * Hua Zhang View author publications You can also


search for this author inPubMed Google Scholar * Tao Ren View author publications You can also search for this author inPubMed Google Scholar * Qian Shen View author publications You can


also search for this author inPubMed Google Scholar * Kai Wang View author publications You can also search for this author inPubMed Google Scholar * Yingyong Hou View author publications


You can also search for this author inPubMed Google Scholar * Xuefeng Xia View author publications You can also search for this author inPubMed Google Scholar * Xingxiang Pu View author


publications You can also search for this author inPubMed Google Scholar * David P. Carbone View author publications You can also search for this author inPubMed Google Scholar * Xin Zhang


View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS Prof. Xin Zhang is the guarantor of the content of the manuscript, including the data and


analysis. Xinyu Zhang and YX had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. ZY, Yencheng Chao,


QH, CL, MY, X Zhu, LC, JB, Y Gong, Y Guan, MZ, JH, HZ, TR, QS, KW, YH, XX, DPC and XP contributed substantially to the study design, data analysis and interpretation, and the writing of the


manuscript. CORRESPONDING AUTHORS Correspondence to Xingxiang Pu, David P. Carbone or Xin Zhang. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. ETHICS


APPROVAL AND CONSENT TO PARTICIPATE This study was approved by the Ethics Committee of the Zhongshan Hospital of Fudan University (No. B2018-027) and complied with all relevant ethical


regulations. All participating patients signed written informed consent. CONSENT TO PUBLISH Not applicable. ADDITIONAL INFORMATION PUBLISHER’S NOTE Springer Nature remains neutral with


regard to jurisdictional claims in published maps and institutional affiliations. SUPPLEMENTARY INFORMATION SUPPLEMENTARY FMATERIAL TABLE S1 TABLE S2 TABLE S3 TABLE S4 TABLE S5 TABLE S6


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CITE THIS ARTICLE Zhang, X., Yu, Z., Xu, Y. _et al._ Utility of cell-free DNA from bronchial washing fluid in diagnosis and genomic determination for radiology-suspected pulmonary nodules.


_Br J Cancer_ 127, 2154–2165 (2022). https://doi.org/10.1038/s41416-022-01969-2 Download citation * Received: 12 July 2021 * Revised: 05 July 2022 * Accepted: 23 August 2022 * Published: 17


October 2022 * Issue Date: 07 December 2022 * DOI: https://doi.org/10.1038/s41416-022-01969-2 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content:


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