Runx1-it1 acts as a scaffold of stat1 and nurd complex to promote ros-mediated nf-κb activation and ovarian cancer progression

ABSTRACT Dysregulated expression of long-stranded non-coding RNAs is strongly associated with carcinogenesis. However, the precise mechanisms underlying their involvement in ovarian cancer

pathogenesis remain poorly defined. Here, we found that lncRNA RUNX1-IT1 plays a crucial role in the progression of ovarian cancer. Patients with high RUNX1-IT1 expression had shorter

survival and poorer outcomes. Notably, knockdown of RUNX1-IT1 suppressed the proliferation, migration and invasion of ovarian cancer cells in vitro, and reduced the formation of peritoneum

metastasis in vivo. Mechanistically, RUNX1-IT1 bound to HDAC1, the core component of the NuRD complex, and STAT1, acting as a molecular scaffold of the STAT1 and NuRD complex to regulate

intracellular reactive oxygen homeostasis by altering the histone modification status of downstream targets including GPX1. Consequently, RUNX1-IT1 activated NF-κB signaling and altered the

biology of ovarian cancer cells. In conclusion, our findings demonstrate that RUNX1-IT1 promotes ovarian malignancy and suggest that targeting RUNX1-IT1 represents a promising therapeutic

strategy for ovarian cancer treatment. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through

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Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS LINC01320 FACILITATES CELL PROLIFERATION AND MIGRATION OF OVARIAN CANCER VIA REGULATING PURB/DDB2/NEDD4L/TGF-Β

AXIS Article Open access 31 October 2024 LNCRNA DNM1P35 SPONGES HSA-MIR-326 TO PROMOTE OVARIAN CANCER PROGRESSION Article Open access 28 December 2024 ETV5-MEDIATED UPREGULATION OF LNCRNA

CTBP1-DT AS A CERNA FACILITATES HGSOC PROGRESSION BY REGULATING MIR-188-5P/MAP3K3 AXIS Article Open access 09 December 2021 DATA AVAILABILITY The data generated in this study are available

within the article and its supplementary information files. The raw data generated in this study are publicly available in Gene Expression Omnibus (GEO) at GSE224495 and GSE245778.

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  PubMed  Google Scholar  Download references FUNDING This research was funded by the National Key R&D Program of China (2021YFC2501000, 2020YFA0803300), the National Natural Science

Foundation of China (82030089, 82188102), the CAMS Innovation Fund for Medical Sciences (2021–1-I2M-018, 2021-I2M-1-067, 2022-I2M-2-001). AUTHOR INFORMATION Author notes * Qingyu Luo & 

Xiaowei Wu Present address: Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, 02215, USA * These authors contributed equally: Xiao Yu, Pengfei Zhao. AUTHORS AND AFFILIATIONS

* State Key Laboratory of Molecular Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union

Medical College, Beijing, 100021, China Xiao Yu, Pengfei Zhao, Qingyu Luo, Xiaowei Wu, Yabing Nan, Shi Liu, Wenyan Gao & Zhihua Liu * Department of Gynecological Oncology, National

Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China Yating Wang & Bin

Li * Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao, 266000, China Zhumei Cui Authors * Xiao Yu View author publications You can also search

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publications You can also search for this author inPubMed Google Scholar * Wenyan Gao View author publications You can also search for this author inPubMed Google Scholar * Bin Li View

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View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS XY and PZ contributed equally to this work. ZL and ZC conceived the ideas and supervised

the research. XY and PZ performed most of the experiments, analyzed the data and drafted the manuscript with assistance from ZL and ZC. WG performed the bioinformatics analyses. QL, XW, YW,

and BL collected clinical samples. SL and YN assisted with animal studies. All authors reviewed the manuscript and agreed to the published version of the manuscript. CORRESPONDING AUTHORS

Correspondence to Zhihua Liu or Zhumei Cui. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. ADDITIONAL INFORMATION PUBLISHER’S NOTE Springer Nature

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permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Yu, X., Zhao, P., Luo, Q. _et al._ RUNX1-IT1 acts as a scaffold of STAT1 and NuRD complex to promote ROS-mediated NF-κB activation and

ovarian cancer progression. _Oncogene_ 43, 420–433 (2024). https://doi.org/10.1038/s41388-023-02910-4 Download citation * Received: 14 June 2023 * Revised: 23 November 2023 * Accepted: 28

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