16 intestinal hem iron uptake is an endocytic-mediated process

ABSTRACT Background: Hem-iron is the main source of iron intake for humans in developed countries, which is best absorbed than inorganic iron (Fe). Iron deficiency is one of the most

prevalent nutritional deficiencies in developing countries. One of the strategies to improve Fe nutrition is to increase hem-Fe intake in the population. Objective: To study in vitro the

mechanisms of hem-Fe uptake and transport at the intestinal cells. Methods: Caco-2 Cells were grown in Iscove′s media, supplemented with 10% SFB, hem-Fe (Hb-Fe55) (0,1–100 uM Fe). We

determined in cellular extracts, total intracellular Fe by AAE and heme oxygenase and DMT1(Divalent Metal Transporter 1) by Western Blot. We studied endocytic process, temperature effect and

hem-Fe uptake and transport. The radioactivity was counted in the cells and in basolateral media. Results: Cells exposed to hem-Fe showed lower intracellular Fe than cells exposed to

inorganic Fe. Hem-Fe uptake was inhibited by acidification and K+ depletion. The highest uptake and transport were observed in cells incubated with hem-Fe. Heme oxygenase 1 expression

increased and DMT1 expression decreased when hem-Fe increased in the incubation media. Conclusions: Hem-Fe uptake in the intestinal epithelia cells would be by a inorganic Fe independent

mechanism by a saturable and endocytic process dependent. Financing: FONDECYT 1030633 Chile. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Laboratorio de Micronutrientes,

Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile M Arredondo, S Flores, M J Mendiburo, S Núñez, M Olivares & F Pizarro Authors * M Arredondo

View author publications You can also search for this author inPubMed Google Scholar * S Flores View author publications You can also search for this author inPubMed Google Scholar * M J

Mendiburo View author publications You can also search for this author inPubMed Google Scholar * S Núñez View author publications You can also search for this author inPubMed Google Scholar

* M Olivares View author publications You can also search for this author inPubMed Google Scholar * F Pizarro View author publications You can also search for this author inPubMed Google

Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Arredondo, M., Flores, S., Mendiburo, M. _et al._ 16 Intestinal HEM Iron Uptake is an

Endocytic-Mediated Process. _Pediatr Res_ 57, 922 (2005). https://doi.org/10.1203/00006450-200506000-00044 Download citation * Issue Date: 01 June 2005 * DOI:

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