Surfactant distribution in the piglet model of meconium aspiration. † 1284

_OBJECTIVE:_ This study compares the distribution of exogenous pulmonary surfactant in piglets with and without meconium instillation. Eight 2-4 kg Yorkshire-type piglets were intubated and

supported with positive pressure ventilation (PPV) and oxygen to maintain inspiratory tidal volumes of approximately 10 ml/kg and oxygen saturations of > 95%. Each piglet was randomized

to receive either meconium followed one hour later by surfactant(MS) or surfactant only (SO). In the MS group 5 ml/kg of a 30% solution of human meconium was delivered via a feeding tube in

two divided doses with the piglet in the right then left lateral position. 4 ml/kg 3H-DPPC radiolabeled surfactant was delivered in the same manner to all animals. The pigs were euthanized 4

hours after surfactant instillation in both groups. The lungs were dissected, frozen, and later sectioned into pieces with a mass of 0.71 ± 0.14 g (mean ± 1 SD). The location and mass of

each piece were recorded. Tritium counts were determined by liquid scintillation and the density of surfactant for each piece was calculated by count/mass ratio. Data were standardized as

multiples of the mean (MoM) and plotted as histograms. The variances were compared by the F-test. ANOVA compared density of surfactant by lobes. _Results:_ The histograms indicate

heterogeneous distribution of surfactant in both groups, with more heterogeneity of distribution in the MS group (p<0.002). When mean surfactant density by lobe is considered, there are

no significant differences between the two groups. _Conclusions:_ Tracheal instillation of meconium in this model significantly increased the heterogeneity of surfactant distribution.

Meconium aspiration results in heterogeneous lung mechanics and surfactant therapy may exacerbate this heterogeneity. This study was funded in part by Ross Products Division, Abbott

Laboratories. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Wilford Hall USAF Medical Center, Lackland AFB, TX., Maureen L Hudson, David S Louder & Richard F Stribley Authors * Maureen L

Hudson View author publications You can also search for this author inPubMed Google Scholar * David S Louder View author publications You can also search for this author inPubMed Google

Scholar * Richard F Stribley View author publications You can also search for this author inPubMed Google Scholar ADDITIONAL INFORMATION (Spon. by Robert A. deLemos). RIGHTS AND PERMISSIONS

Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Hudson, M., Louder, D. & Stribley, R. SURFACTANT DISTRIBUTION IN THE PIGLET MODEL OF MECONIUM ASPIRATION.† 1284. _Pediatr

Res_ 39 (Suppl 4), 217 (1996). https://doi.org/10.1203/00006450-199604001-01307 Download citation * Issue Date: 01 April 1996 * DOI: https://doi.org/10.1203/00006450-199604001-01307 SHARE

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