Immunoreactive somatomedin c/insulin-like growth factor i in urine from normal subjects, pituitary dwarfs, and acromegalics

ABSTRACT ABSTRACT: Using antibodies to somatomedin C/insulin-like growth factor I (SmC) produced in rabbits using the recombinant hormone, we have developed a radioimmuno-assay for SmC.

Gel-chromatography of urine revealed that the vast majority of immunoreactive SmC was eluted coincident with 125I-SmC and a small portion eluted with fractions having a mol. wt. range of

30,000–40,000. The SmC concentration in urine was determined by radioimmuno-assay after ammonium sulfate extraction. Values did not ordinarily exceed 1 ng/ml. When the values from normal

subjects were expressed as ng/mg creatinine, high levels were observed in the neonatal period. These values fell rapidly in infancy, declined more gradually in childhood, were slightly

elevated at early puberty, and were lowest in adulthood. Urine SmC concentrations in 15 pituitary dwarfs were lower than the averages obtained from age-matched control subjects, and six of

them showed abnormally low values. Three patients with active acromegaly had high SmC values in urine. In conclusion, 1) SmC, mainly of monomeric form, was immunologically detected in urine.

2) Radioimmunoassay for urine SmC revealed that values varied considerably with age in normal subjects and were partially dependent on the human growth hormone status. However, the full

meaning of the findings remains to be elucidated. SIMILAR CONTENT BEING VIEWED BY OTHERS NESFATIN-1 AND NESFATIN-1-LIKE PEPTIDE SUPPRESS GROWTH HORMONE SYNTHESIS VIA THE AC/PKA/CREB PATHWAY

IN MAMMALIAN SOMATOTROPHS Article Open access 07 October 2020 INSULIN-LIKE GROWTH FACTOR-1 LEVEL IS A POOR DIAGNOSTIC INDICATOR OF GROWTH HORMONE DEFICIENCY Article Open access 09 August

2021 PLASMA AND SERUM PRORENIN CONCENTRATIONS IN DIABETES, HYPERTENSION, AND RENAL DISEASE Article 10 June 2022 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of

Pediatrics, Kanagawa Children's Medical Center, Yokohama Susumu Yokoya, Seizo Suwa, Hatae Maesaka & Toshiaki Tanaka * Department of Endocrinology and Metabolism, National

Children's Medical Research Center, Tokyo, Japan Susumu Yokoya, Seizo Suwa, Hatae Maesaka & Toshiaki Tanaka Authors * Susumu Yokoya View author publications You can also search for

this author inPubMed Google Scholar * Seizo Suwa View author publications You can also search for this author inPubMed Google Scholar * Hatae Maesaka View author publications You can also

search for this author inPubMed Google Scholar * Toshiaki Tanaka View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Yokoya, S., Suwa, S., Maesaka, H. _et al._ Immunoreactive Somatomedin C/Insulin-Like Growth Factor I in Urine from Normal Subjects, Pituitary

Dwarfs, and Acromegalics. _Pediatr Res_ 23, 151–154 (1988). https://doi.org/10.1203/00006450-198802000-00002 Download citation * Received: 24 July 1987 * Accepted: 23 September 1987 * Issue

Date: 01 February 1988 * DOI: https://doi.org/10.1203/00006450-198802000-00002 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable

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