Ontogeny of cell type-specific enzyme reactivities in kidney collecting ducts

ABSTRACT ABSTRACT. Highly specific antibodies against vital enzymes of the collecting ducts were used to study the appearance of cell type specific enzyme profiles in developing rat kidneys.

(Na+K)-ATPase, the abundant enzyme of principal cells, could be detected early _in utero_ in most collecting duct cells. However, the characteristic basolateral polarization of this enzyme

did not appear until the first hours after birth. After this, the relative amount of (Na+K)-ATPase immunoreactive cells along collecting ducts decreased steadily, to reach the amount found

in adult rat kidneys by the 30th postnatal day. Carbonic anhydrase immunoreactivity characteristic for intercalated cells was not detectable in fetal kidneys, but appeared soon after birth,

with steadily increasing numbers of cells that were positive. Interestingly, immunoreactive band 3 glycoprotein (anion channel protein of erythrocytes) did not appear until the 5th day of

life, with only a slowly increasing number of cells positive for this probe. These results, showing the sequential appearance of cell type-specific enzyme reactivities along collecting

ducts, likely reflect a similar pattern of functional development of the respective main cell types. These results may provide an explanation for physiologic neonatal acidosis, as the enzyme

profile associated with proton secretion was seen to appear slowly during the first weeks of life in a distinct manner. SIMILAR CONTENT BEING VIEWED BY OTHERS URINARY REABSORPTION IN THE

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STRUCTURE AND THIAZIDE INHIBITION MECHANISM OF THE HUMAN NA–CL COTRANSPORTER Article 15 February 2023 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Bacteriology

and Immunology, University of Helsinki, Helsinki, Finland Harry Holthöfer Authors * Harry Holthöfer View author publications You can also search for this author inPubMed Google Scholar

RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Holthöfer, H. Ontogeny of Cell Type-Specific Enzyme Reactivities in Kidney Collecting Ducts. _Pediatr

Res_ 22, 504–508 (1987). https://doi.org/10.1203/00006450-198711000-00005 Download citation * Received: 24 February 1987 * Accepted: 03 June 1987 * Issue Date: 01 November 1987 * DOI:

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