Effect of hormone administration on the sialylation and fucosylation of intestinal microvillus membranes of suckling rats

ABSTRACT ABSTRACT: Cortisone, thyroxine, epidermal growth factor, or insulin were administered to 8-day-old rats for 4 days. In comparison to saline-injected controls, cortisone treatment:

1) lowered the sialic acid and raised the fucose content of the intestinal microvillus membranes, 2) increased [3H]fucose incorporation into these membranes, and 3) decreased the membrane

binding of 125I-wheat germ agglutinin, while increasing the binding of 125I-ulex europeus agglutinin I and 125I-peanut agglutinin. Thyroxine treatment had similar effects on fucose content

and 125Iulex europeus agglutinin I binding, but did not alter [3H] fucose incorporation or sialic acid content. At the doses used, epidermal growth factor and insulin had no significant

effects. The effect of cortisone treatment on sialic acid and fucose was commensurate with a 5- to 6-day acceleration of postnatal intestinal maturation. The changes in lectin binding,

however, suggested qualitative differences between developmental and cortisone-induced membrane glycosylation. In addition, this study demonstrates significant quantitative and qualitative

differences in the response of intestinal glycosylation to pharmacologic doses of the four hormones. SIMILAR CONTENT BEING VIEWED BY OTHERS LECTIN HISTOCHEMISTRY OF POSTERIOR LINGUAL GLANDS

OF DEVELOPING RATS Article Open access 26 June 2023 TAMM–HORSFALL PROTEIN IN HUMANE URINE: SEX-DEPENDENT DIFFERENCES IN THE EXCRETION AND _N_-GLYCOSYLATION PATTERN Article Open access 19

October 2023 PREGNANCY-RELATED CHANGES IN THE CANINE SERUM N-GLYCOSYLATION PATTERN STUDIED BY RAPIFLUOR HILIC-UPLC-FLR-MS Article Open access 06 September 2024 ARTICLE PDF AUTHOR INFORMATION

AUTHORS AND AFFILIATIONS * Department of Pediatrics, Pediatric Gastroenterology and Nutrition Section, University of Oklahoma Health Sciences Center, Oklahoma City, 73126, Oklahoma Akhtar

Mahmood & Ramon Torres-Pinedo Authors * Akhtar Mahmood View author publications You can also search for this author inPubMed Google Scholar * Ramon Torres-Pinedo View author publications

You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Mahmood, A., Torres-Pinedo, R. Effect of

Hormone Administration on the Sialylation and Fucosylation of Intestinal Microvillus Membranes of Suckling Rats. _Pediatr Res_ 19, 899–902 (1985).

https://doi.org/10.1203/00006450-198509000-00005 Download citation * Received: 14 January 1985 * Accepted: 15 April 1985 * Issue Date: 01 September 1985 * DOI:

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