274 drenergic receptor in mamalian placenta: comparison of rat, guinea pig, rabbit, human and trophoblastic tumors

ABSTRACT β-Adrenergic receptor (βAR) has been previously demonstrated in human placental membranes. Its demonstration in placentas of multiple species and trophoblastic tumors might support

its regulatory function on the trophoblastic cell itself. βAR in rat, guinea pig, rabbit and human placenta and trophoblastic tumors were therefore examined. Term placentas were obtained,

dissected to remove decidua and processed in identical fashion. The β-adrenergic antagonist dihydroalprenolol, (−)[3H]DHA bound to a single, saturable class of sites in all placental

membranes. Placental βAR was coupled with catecholamine sensitive adenylate cyclase in all species. βAR was absent in two human choriocarcinoma cell lines (JEG-3, BeWo) but was present in a

hydatidiform mole obtained freshly from surgery (91 fmoles/mg, KD=1.31 nM). We conclude that 1) variable but consistently high density βAR is present coupled to adenylate cyclase in

mammalian placenta; 2) the affinity (KD) of the βAR is similar in all species; and 3) the presence of high density βAR on a hydatidiform mole containing almost totally trophoblast tissue

suggests a βAR is located on trophoblastic cell membranes. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * U. of Cincinnati Coll. of Med., Dept. Peds., John Moore & Jeffrey A

Whitsett Authors * John Moore View author publications You can also search for this author inPubMed Google Scholar * Jeffrey A Whitsett View author publications You can also search for this

author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Moore, J., Whitsett, J. 274 DRENERGIC RECEPTOR IN MAMALIAN PLACENTA:

COMPARISON OF RAT, GUINEA PIG, RABBIT, HUMAN AND TROPHOBLASTIC TUMORS. _Pediatr Res_ 15 (Suppl 4), 485 (1981). https://doi.org/10.1203/00006450-198104001-00285 Download citation * Issue

Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-00285 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link

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