1002 quantitative protection studies in a suckling pat model of group b streptccoccal sepsis

ABSTRACT Group B streptococci (GBS) are a major cause of sepsis and meningitis in the newborn. Neonatal susceptibility to GBS infection appears to be associated with a deficiency of opsonic

antibody to GBS. The present studies were designed to determine if human antibodies protective for one type III GBS strain provide uniform protection for other type III strains. IgG was

affinity purified from pooled human immunoglobulin using a staphylococcal protein A immunoabsorbant. The relative titers of IgG antibodies to group B and type III (native and core) antigens

were determined. To evaluate protection, suckling rats were challenged S.Q. with each of 4 different clinical isolates of GBS type III followed immediately by various concentrations of

purified IgG given IP. With 5 or 10 μg of IgG significant survival occurred in animals challenged with one strain (17/20, 85%), whereas this amount of antibody afforded no protection against

the other 3 strains (0/27). Administration of 20 μg of IgG provided some protection for 2 of these strains (7/23, 3/9), but uniform lethality occurred with the fourth strain even when more

than 40 μg IgG was given. These studies demonstrate that four type III GBS strains require different amounts of human IgG for protection. Strain variability must be examined in future

investigations of vaccine induced or passively acquired IgG for protection against GBS disease. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pediatrics, Uniformed

Services University of the Health Sciences, Bethesda, 20014, MD. Gerald W Fischer, Samuel R Wilson, Kenneth W Hunter & Val G Hemming Authors * Gerald W Fischer View author publications

You can also search for this author inPubMed Google Scholar * Samuel R Wilson View author publications You can also search for this author inPubMed Google Scholar * Kenneth W Hunter View

author publications You can also search for this author inPubMed Google Scholar * Val G Hemming View author publications You can also search for this author inPubMed Google Scholar RIGHTS

AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Fischer, G., Wilson, S., Hunter, K. _et al._ 1002 QUANTITATIVE PROTECTION STUDIES IN A SUCKLING PAT MODEL OF

GROUP B STREPTCCOCCAL SEPSIS. _Pediatr Res_ 15 (Suppl 4), 609 (1981). https://doi.org/10.1203/00006450-198104001-01028 Download citation * Issue Date: 01 April 1981 * DOI:

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