809 detection and characterization of erythropoietic inhibitors in anemia of chronic renal failure

ABSTRACT The mechanism of anemia in pts with end-stage renal disease was studied by assessing erythroid colony growth in methylcellulose cultures. Peripheral blood BFU-E from 10 anemic pts

were normal when cultured in control serum (mean 22 BFU-E/105, range 14-41 vs control mean 18±10/105), but declined a mean of 67% when autologous uremic serum was substituted. Sera from 53

of 60 pts cultured with control marrow produced a mean decrease in BFU-E of 74% and in CFU-E of 79%. The serum inhibition was confirmed by reproducing the effect on control marrow colony

growth with specific fractions of pts' serum separated by sephacryl gel chromatography. Neither peritoneal dialysis nor hemodialysis reduced the inhibitory activity, but it disappeared

with successful renal transplantation. Analysis of uremic serum revealed a striking increase in a ribonuclease of M.W. 33,000 in all pts (9,500-40,000u/ml vs control mean 1,047±247u/ml) that

was not eliminated by therapeutic dialysis. Purified ribonuclease produced dose-dependent inhibition of control marrow CFU-E but had unpredictable effects on BFU-E. We conclude that in

anemic uremic pts: erythroid progenitors are adequate; the serum contains erythropoietic inhibitors; a ribonuclease is increased and appears to have a role in the erythropoietic suppression.

ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Univ. of Toronto, Hosp for Sick Children, Div of Hematology, Toronto, Canada Melvin H Freedman, Tom Grunberger, E Fred Saunders, H

Michael Dosch, Daniel C Cattran & Eli Z Rabin Authors * Melvin H Freedman View author publications You can also search for this author inPubMed Google Scholar * Tom Grunberger View

author publications You can also search for this author inPubMed Google Scholar * E Fred Saunders View author publications You can also search for this author inPubMed Google Scholar * H

Michael Dosch View author publications You can also search for this author inPubMed Google Scholar * Daniel C Cattran View author publications You can also search for this author inPubMed 

Google Scholar * Eli Z Rabin View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS

ARTICLE Freedman, M., Grunberger, T., Saunders, E. _et al._ 809 DETECTION AND CHARACTERIZATION OF ERYTHROPOIETIC INHIBITORS IN ANEMIA OF CHRONIC RENAL FAILURE. _Pediatr Res_ 15 (Suppl 4),

577 (1981). https://doi.org/10.1203/00006450-198104001-00834 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-00834 SHARE THIS ARTICLE Anyone

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