Androgen receptor levels in preputial skin from boys with hypospadias

ABSTRACT The level of high-affinity low capacity binding of [3H] methyltrienolone ([3H]MT) to human preputial skin cytosol in 7 boys with hypospadias and in 7 control subjects have been

measured.[3H]MT has been shown to have a specific affinity to the androgen receptor. The binding component was heat-labile, was totally destroyed by trypsin and the degradation at 0°C was

slow (t 1/2=70 h). The time of dissociation of the 3H MT-receptor complex at 0°C was slow (t 1/2=55 h). DHT and testosterone displaced [3H] MT from the receptor whereas androstendione,

progesterone, estradiol and R 5020 were much less effective competitors and cortisol did not compete. The dissociation constant of the 3H MT-receptor complex varied between 0.17-2.17×10-10M

and the number of binding sites between 1.08-7.35 fmoles/mg protein. No significant differences were found between boys with hypospadias and control subjects. Procedure of collecting skin

samples was approved by the Ethical Committee of Karolinska Sjukhuset. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Depts. of Pediatric Surgery and Pediatric Endocrine Unit,

Karolinska Sjukhuset Dept. of Chemistry, Karolinska Institutet, Stockholm, S-104 01, Sweden J Svensson & M Snochowski Authors * J Svensson View author publications You can also search

for this author inPubMed Google Scholar * M Snochowski View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Svensson, J., Snochowski, M. Androgen receptor levels in preputial skin from boys with hypospadias. _Pediatr Res_ 12, 1093 (1978).

https://doi.org/10.1203/00006450-197811000-00074 Download citation * Issue Date: 01 November 1978 * DOI: https://doi.org/10.1203/00006450-197811000-00074 SHARE THIS ARTICLE Anyone you share

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