Lamellar bodies and surfactant phospholipids after pulmonary artery occlusion and reperfusion

ABSTRACT 22 dogs were anesthetized (sodium pentabarbital), intubated with a Carlens double lumen endotracheal tube and ventilated mechanically (Vt=15ml/kg) in the supine position. Nine

animals underwent 2 to 24 hrs of balloon left pulmonary artery occlusion (LPAO), 8 had LPAO of 2 to 12 hrs followed by 6 hrs of reperfusion, and 5 served as controls. PaCO2, pH and PaO2 were

maintained in the normal range. At necropsy the right and left lungs were lavaged with 0.9% NaCl and tissue was obtained for phospholipid analysis and electron microscopy (EM).

Phospholipids were separated by 2 dimensional thin layer chromatography on silica gel H. Phosphatidylcholine, phosphatidyiserine, phosphatidyl inositol, phosphatidylethanolamine,

phosphatidylglycerol and sphingomyelin were measured as phosphate and by densitometry. Results: 1. No animal developed atelectasis. 2. There were no qualitative differences in phospholipid

composition, either between study animals and controls or between non-occluded, occluded or reperfused lungs of the same animal. 3. Depletion of lamellar bodies (LB) from type 11 pneumocytes

within 2 hrs of LPAO, followed by reappearance in 12-24 hrs was demonstrated by EM. 4. Six hrs of reperfusion was associated with reappearance of LB. We conclude that LPAO produces

temporary cellular depletion of LB lasting from 2 to 12 hrs and that no qualitative alterations in the phospholipid composition of tissue or alveolar wash result from LPAO. ARTICLE PDF

AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * department of Pediatrics, University of California, San Diego, La Jolla Brian S Saunders, John W Sheoard Jr. & Louis Gluck Authors * Brian S

Saunders View author publications You can also search for this author inPubMed Google Scholar * John W Sheoard Jr. View author publications You can also search for this author inPubMed 

Google Scholar * Louis Gluck View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS

ARTICLE Saunders, B., Sheoard, J. & Gluck, L. LAMELLAR BODIES AND SURFACTANT PHOSPHOLIPIDS AFTER PULMONARY ARTERY OCCLUSION AND REPERFUSION. _Pediatr Res_ 11, 578 (1977).

https://doi.org/10.1203/00006450-197704000-01249 Download citation * Issue Date: 01 April 1977 * DOI: https://doi.org/10.1203/00006450-197704000-01249 SHARE THIS ARTICLE Anyone you share the

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