Identifying lrrc16b as an oncofetal gene with transforming enhancing capability using a combined bioinformatics and experimental approach

ABSTRACT Oncofetal genes are expressed in embryos or fetuses, are downregulated or undetectable in adult tissues, and then re-expressed in tumors. Known oncofetal genes, such as _AFP_,

_GCB_, _FGF18_, _IMP-1_ and _SOX1_, often have important clinical applications or pivotal biological functions. To find new oncofetal-like genes, we used the public information of expressed

sequence tags to systematically analyze gene expression patterns and identified a novel oncofetal-like gene, _LRRC16B_. It increased the proliferation, anchorage-independent growth and

tumorigenesis of transformed cells in xenografts, possibly through its effects on cyclin B1 protein levels. These findings exemplify the feasibility of using bioinformatics to find new

oncofetal-like genes and suggest that more genes with important functional roles will be uncovered in the candidate gene list. Access through your institution Buy or subscribe This is a

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integration of cancer microarray data identifies a robust common cancer signature. _BMC Bioinform_ 8: 275. Article  Google Scholar  Download references ACKNOWLEDGEMENTS This was supported by

Grant NSC-95-2320-B-006-057-MY2 from the National Science Council, Taiwan, and Grant DOH99-TD-C-111-003 from the Department of Health, Taiwan. There is no competing financial interest in

relation to this work. AUTHOR INFORMATION Author notes * C-W Chiang, H-C Cheng and W-T Chang: These authors contributed equally to this work. AUTHORS AND AFFILIATIONS * Institute of Basic

Medical Science, College of Medicine, National Cheng Kung University, Tainan, Taiwan C-C Hsu, C-W Chiang, H-C Cheng, W-T Chang, T-Y Lee, N-H Chow & C-L Ho * Institute of Molecular

Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan C-W Chiang, Z-H Wu, N-H Chow & C-L Ho * Department of Obstetrics and Gynecology, National Cheng Kung

University Hospital, Tainan, Taiwan C-Y Chou * Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan H-W Tsai, C-T Lee, N-H Chow & C-L Ho * Department of

Ophthalmology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan A Chao Authors * C-C Hsu View author publications You can also search for this author

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DECLARATIONS COMPETING INTERESTS The authors declare no conflict of interest. ADDITIONAL INFORMATION Supplementary Information accompanies the paper on the Oncogene website SUPPLEMENTARY

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50 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Hsu, CC., Chiang, CW., Cheng, HC. _et al._ Identifying _LRRC16B_ as an oncofetal gene with

transforming enhancing capability using a combined bioinformatics and experimental approach. _Oncogene_ 30, 654–667 (2011). https://doi.org/10.1038/onc.2010.451 Download citation * Received:

07 February 2010 * Revised: 09 August 2010 * Accepted: 24 August 2010 * Published: 22 November 2010 * Issue Date: 10 February 2011 * DOI: https://doi.org/10.1038/onc.2010.451 SHARE THIS

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Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * oncofetal genes * expressed sequence tags * _LRRC16B_