Solution structure of a mammalian pcb-binding protein in complex with a pcb

ABSTRACT Metabolites of polychlorinated biphenyls (PCBs) bind with high affinity to uteroglobin, a small homodimeric protein that also binds progesterone. We present the solution structure

of the reduced form of rat uteroglobin in complex with a PCB methylsulphone, (MeSO2)2-TCB. The structure reveals the molecular basis for the accumulation of (MeSO2)2-TCB by uteroglobin. The

structure also shows how ligand binding and release might be controlled by reduction/oxidation of two intermolecular disulphide bonds. Breakage of these bonds induces a local unfolding of

the N- and C-termini and a separation of helices creating a channel into the binding site. These effects make the ligand binding cavity readily accessible to entry of the ligand. Access

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SIMILAR CONTENT BEING VIEWED BY OTHERS BIOCOORDINATION REACTIONS IN COPPER(II) IONS AND PHOSPHOCHOLINE SYSTEMS INCLUDING PYRIMIDINE NUCLEOSIDES AND NUCLEOTIDES Article Open access 04 July

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REVEALS CATALYTIC CYCLE DYNAMICS DURING NON-NATIVE SUBSTRATE OXIDATION BY CYTOCHROME P450BM3 Article Open access 12 March 2025 REFERENCES * Safe, S., Toxicology, structure-function

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Graphics_ 6, 219–220 (1988). Article  Google Scholar  Download references AUTHOR INFORMATION Author notes * Henry J. Barnes Present address: Immune Complex Corporation, 11099 N.Torrey Pines

Road Suite 240, La Jolla, California, 92037, USA AUTHORS AND AFFILIATIONS * Center for Structural Biochemistry, Karolinska Institutet NOVUM, S-14157, Huddinge, Sweden Torleif Härd *

Department of Medical Nutrition, Karolinska Institutet NOVUM, S-14157, Huddinge, Sweden Henry J. Barnes, Jan-Åke Gustafsson & Johan Lund * Department of Environmental Chemistry,

Wallenberg Laboratory, Stockholm University, S-106 91, Stockholm, Sweden Christina Larsson Authors * Torleif Härd View author publications You can also search for this author inPubMed Google

Scholar * Henry J. Barnes View author publications You can also search for this author inPubMed Google Scholar * Christina Larsson View author publications You can also search for this

author inPubMed Google Scholar * Jan-Åke Gustafsson View author publications You can also search for this author inPubMed Google Scholar * Johan Lund View author publications You can also

search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Härd, T., Barnes, H., Larsson, C. _et al._ Solution

structure of a mammalian PCB-binding protein in complex with a PCB. _Nat Struct Mol Biol_ 2, 983–989 (1995). https://doi.org/10.1038/nsb1195-983 Download citation * Received: 11 August 1995

* Accepted: 20 September 1995 * Issue Date: 01 November 1995 * DOI: https://doi.org/10.1038/nsb1195-983 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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