Ctcf-binding site orientation shapes the genome

Access through your institution Buy or subscribe The protocadherin-α (_PCDHA_) and protocadherin-γ (_PCDHG_) gene clusters contain dozens of alternatively expressed exons, most of which are

preceded by a CBS-containing promoter. Using various chromosome conformation capture techniques, the authors found that the _PCDH_ clusters are organized into two TAD-like,

CTCF–cohesin-mediated chromatin domains. The CBS-containing _HS51_ enhancer, which lies between the _PCDHA_ and _PCDHG_ clusters, interacts with only the _PCDHA_ cluster promoters in human

cells and in mouse brain tissues and cells, whereas a newly identified enhancer (located downstream of the _PCDH_ locus) interacts with the _PCDHG_ cluster promoters. Importantly, the

promoter CBSs were found to be in a forward orientation, whereas the enhancer CBSs were in the reverse orientation. > the orientation of enhancer CBSs is important for the > 

three-dimensional architecture of the genome and for gene regulation This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution

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Contact customer support REFERENCES * Guo, Y. et al. CRISPR inversion of CTCF sites alters genome topology and enhancer/promoter function. _Cell_ 162, 900–910 (2015) Article  CAS  Google

Scholar  Download references Authors * Eytan Zlotorynski View author publications You can also search for this author inPubMed Google Scholar RELATED LINKS RELATED LINKS RELATED LINKS IN

NATURE RESEARCH Pombo, A. & Dillon, N. Three-dimensional genome architecture: players and mechanisms. _Nat. Rev. Mol. Cell Biol._ 16, 245–257 (2015) RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Zlotorynski, E. CTCF-binding site orientation shapes the genome. _Nat Rev Mol Cell Biol_ 16, 578–579 (2015). https://doi.org/10.1038/nrm4057

Download citation * Published: 26 August 2015 * Issue Date: October 2015 * DOI: https://doi.org/10.1038/nrm4057 SHARE THIS ARTICLE Anyone you share the following link with will be able to

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