Going in circles | Nature Reviews Molecular Cell Biology

Access through your institution Buy or subscribe The authors introduced into fruit fly DL1 cells a three-exon minigene derived from the laccase2 gene, which is known to produce circRNA.

Depletion of several spliceosome proteins largely eliminated the expression of the minigene linear mRNA. By contrast, expression of the minigene circRNA in these cells was only slightly

decreased or even increased. Importantly, the splicing patterns of the endogenous laccase2 gene and of two other circRNA-producing genes were similarly affected. Furthermore, the depletion

of 25 other core spliceosome proteins resulted in increase in circRNA expression from various endogenous genes. These data suggested that the relative efficiency of back-splicing compared

with canonical splicing increases when the quantity of spliceosome proteins is limited. > failure to terminate transcription can result in circRNA production > from downstream genes

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ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Liang, D. et al. The output of protein-coding genes shifts to

circular RNAs when the pre-mRNA processing machinery is limiting. _Mol. Cell_ http://dx.doi.org/10.1016/j.molcel.2017.10.034 (2017) Download references Authors * Eytan Zlotorynski View

author publications You can also search for this author inPubMed Google Scholar RELATED LINKS RELATED LINKS RELATED LINKS IN NATURE RESEARCH Chen, L. L. The biogenesis and emerging roles of

circular RNAs. _Nat. Rev. Mol. Cell Biol._ 17, 205–211 (2016) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Zlotorynski, E. Going in circles. _Nat Rev

Mol Cell Biol_ 19, 3 (2018). https://doi.org/10.1038/nrm.2017.128 Download citation * Published: 06 December 2017 * Issue Date: January 2018 * DOI: https://doi.org/10.1038/nrm.2017.128 SHARE

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