Mapping the mouse mind | Nature Reviews Genetics

Access through your institution Buy or subscribe The GENSAT project exploits the library of bacterial artificial chromosomes (BACs) associated with the mouse genome sequence. Modified BACs

are produced in which a reporter sequence that encodes an enhanced green fluorescent protein (EGFP) is substituted for the coding region of a gene of interest. Transgenic mouse lines are

then generated that carry the construct, which allows reporter-gene expression to be systematically analysed during development. The resulting data, which have been verified through mRNA _in

situ_ hybridization and immunohistochemistry, have been integrated and analysed to create a digital atlas of the mouse brain that can be viewed online and will be updated whenever new data

are generated. This innovative project is allowing gene expression to be correlated with cell type and the elaborate specializations of morphologically complex cells, such as neurons, to be

visualized. Furthermore, small groups of cells that express a particular gene can be identified and their development followed over time. This is a preview of subscription content, access

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this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: *

Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES ORIGINAL RESEARCH PAPER * Gong, S. et al. A gene expression atlas of the central

nervous system based on bacterial artificial chromosomes. _Nature_ 425, 917–925 (2003) Article  CAS  Google Scholar  FURTHER READING * Heintz, N. BAC to the future: the use of BAC transgenic

mice for neuroscience research. _Nature Rev. Neurosci._ 3, 861–870 (2001) Article  Google Scholar  Download references Authors * Victoria Kitchener View author publications You can also

search for this author inPubMed Google Scholar RELATED LINKS RELATED LINKS WEB SITE GENSAT BAC Transgenic Project RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS

ARTICLE Kitchener, V. Mapping the mouse mind. _Nat Rev Genet_ 4, 931 (2003). https://doi.org/10.1038/nrg1235 Download citation * Issue Date: 01 December 2003 * DOI:

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