Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation

ABSTRACT Targeted ectopic expression of genes in the adult brain is an invaluable approach for studying many biological processes. This can be accomplished by generating transgenic mice or

by virally mediated gene transfer, but these methods are costly and labor intensive. We devised a rapid strategy that allows localized _in vivo_ transfection of plasmid DNA within the adult

neurogenic niches without detectable brain damage. Injection of plasmid DNA into the ventricular system or directly into the hippocampus of adult mice, followed by application of electrical

current via external electrodes, resulted in transfection of neural stem or progenitor cells and mature neurons. We showed that this strategy can be used for both fate mapping and gain- or

loss-of-function experiments. Using this approach, we identified an essential role for cadherins in maintaining the integrity of the lateral ventricle wall. Thus, _in vivo_ electroporation

provides a new approach to study the adult brain. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS

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institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS GLIAL CELL TYPE-SPECIFIC GENE EXPRESSION IN THE MOUSE CEREBRUM USING THE

_PIGGYBAC_ SYSTEM AND IN UTERO ELECTROPORATION Article Open access 01 March 2021 EFFICIENT GENERATION OF FUNCTIONAL NEURONS FROM MOUSE EMBRYONIC STEM CELLS VIA NEUROGENIN-2 EXPRESSION

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ACKNOWLEDGEMENTS We thank R. Kageyama (Kyoto University) for the gift of the nestin promoter vector30, C. Ibáñez (Karolinska Institutet) for the gift of a BDNF expression plasmid, M.

Wheelock (University of Nebraska Medical Center) for providing the dominant-negative N-cadherin cDNA and K. Fernandes (University of Montreal) for critically reading the manuscript. This

study was supported by grants from the Swedish Research Council, the Swedish Cancer Society, the Foundation for Strategic Research, the Karolinska Institutet, Tobias Stiftelsen and the

European Commission Framework VI Programme, EuroStemCell. F.B.-H. is supported by a postdoctoral fellowship from Canadian Institutes of Health Research. AUTHOR INFORMATION Author notes *

Konstantinos Meletis Present address: Present address: The Picower Institute for Learning and Memory, Massachusetts Institute of Technology, 43 Vassar Street, Cambridge, Massachusetts 02139,

USA., AUTHORS AND AFFILIATIONS * Department of Cell and Molecular Biology, Medical Nobel Institute, Stockholm, SE-171 77, Sweden Fanie Barnabé-Heider, Konstantinos Meletis, Malin Eriksson, 

Olaf Bergmann, Hanna Sabelström, Harald Mikkers & Jonas Frisén * Department of Neuroscience, Box 285, Karolinska Institutet, Stockholm, SE-171 77, Sweden Michael A Harvey * Department of

Molecular Cell Biology and Regenerative Medicine Program, Leiden University Medical Center, P.O. Box 9600, RC Leiden, 2300, The Netherlands Harald Mikkers Authors * Fanie Barnabé-Heider

View author publications You can also search for this author inPubMed Google Scholar * Konstantinos Meletis View author publications You can also search for this author inPubMed Google

Scholar * Malin Eriksson View author publications You can also search for this author inPubMed Google Scholar * Olaf Bergmann View author publications You can also search for this author

inPubMed Google Scholar * Hanna Sabelström View author publications You can also search for this author inPubMed Google Scholar * Michael A Harvey View author publications You can also

search for this author inPubMed Google Scholar * Harald Mikkers View author publications You can also search for this author inPubMed Google Scholar * Jonas Frisén View author publications

You can also search for this author inPubMed Google Scholar CONTRIBUTIONS F.B.-H. designed, performed and analyzed the study and wrote the manuscript; K.M. designed and performed most parts

of the study; M.E. performed and analyzed part of the study (including BAC analysis); O.B. performed part of the study; H.S. performed and analyzed part of the study; M.A.H. performed EEG

analysis; H.M. designed and performed part of the adenoviral study; and J.F. designed the study and wrote the manuscript. CORRESPONDING AUTHOR Correspondence to Jonas Frisén. SUPPLEMENTARY

INFORMATION SUPPLEMENTARY TEXT AND FIGURES Supplementary Figures 1–3, Supplementary Methods (PDF 435 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE

Barnabé-Heider, F., Meletis, K., Eriksson, M. _et al._ Genetic manipulation of adult mouse neurogenic niches by _in vivo_ electroporation. _Nat Methods_ 5, 189–196 (2008).

https://doi.org/10.1038/nmeth.1174 Download citation * Received: 27 September 2007 * Accepted: 13 December 2007 * Published: 20 January 2008 * Issue Date: February 2008 * DOI:

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