Selective capture of acentric fragments by micronuclei provides a rapid method for purifying extrachromosomally amplified dna

ABSTRACT The amplification and overexpression of a number of oncogenes is strongly associated with the progression of a variety of different cancers. We now present a strategy to purify

amplified DNA on double minute chromosomes (DMs) to enable analysis of their prevalence and contribution to tumourigenesis. Using cell lines derived from four different tumour types, we have

developed a general and rapid method to purify micronuclei that are known to entrap extrachromosomal elements. The isolated DNA is highly enriched in DM sequences and can be used to prepare

probes to localize the progenitor single copy chromosomal regions. The capture of DMs by micronuclei appears to be dependent on their lack of a centromere rather than their small size.

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AUTHORS AND AFFILIATIONS * Gene Expression Laboratory, The Salk Institute for Biological Studies, 10010N. Torrey Pines Road, La Jolla, California, 92037, USA Noriaki Shimizu, Teru Kanda 

& Geoffrey M. Wahl * Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, 724, Japan Noriaki Shimizu Authors * Noriaki Shimizu View author publications You

can also search for this author inPubMed Google Scholar * Teru Kanda View author publications You can also search for this author inPubMed Google Scholar * Geoffrey M. Wahl View author

publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Shimizu, N., Kanda, T. &

Wahl, G. Selective capture of acentric fragments by micronuclei provides a rapid method for purifying extrachromosomally amplified DNA. _Nat Genet_ 12, 65–71 (1996).

https://doi.org/10.1038/ng0196-65 Download citation * Received: 12 June 1995 * Accepted: 18 October 1995 * Issue Date: 01 January 1996 * DOI: https://doi.org/10.1038/ng0196-65 SHARE THIS

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