How evolving enzymes can beat the heat and avoid defeat

Random mutations usually destabilize protein tertiary structure. Overexpression of the chaperonin GroEL/GroES protects evolving proteins from the destabilizing effects of adaptive mutations

and improves the quantity and quality of enzyme variants with modified function. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $259.00 per year only $21.58 per issue Learn more Buy this

article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in

* Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Tokuriki, N. & Tawfik, D.S. _Nature_ 459, 668–673 (2009). Article  CAS  PubMed  Google

Scholar  * Bloom, J.D., Labthavikul, S.T., Otey, C.R. & Arnold, F.H. _Proc. Natl. Acad. Sci. USA_ 103, 5869–5874 (2006). Article  CAS  PubMed  Google Scholar  * Bershtein, S., Goldin, K.

& Tawfik, D.S. _J. Mol. Biol._ 379, 1029–1044 (2008). Article  CAS  PubMed  Google Scholar  * Bloom, J.D. et al. _BMC Biol._ 5, 29 (2007). Article  PubMed  PubMed Central  Google

Scholar  * Rutherford, S.L. _Nat. Rev. Genet._ 4, 263–274 (2003). Article  CAS  PubMed  Google Scholar  * Tomala, K. & Korona, R. _Biol. Direct_ 3, 5 (2008). Article  PubMed  PubMed

Central  Google Scholar  * Ignatova, Z. & Gierasch, L.M. _Proc. Natl. Acad. Sci. USA_ 103, 13357–13361 (2006). Article  CAS  PubMed  Google Scholar  * Panavas, T., Sanders, C. &

Butt, T.R. _Methods Mol. Biol._ 497, 303–317 (2009). Article  CAS  PubMed  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Ichiro Matsumura and Andrei A.

Ivanov are in the Department of Biochemistry, Center for Fundamental and Applied Molecular Evolution, Emory University School of Medicine, Atlanta, Georgia, USA., Ichiro Matsumura & 

Andrei A Ivanov Authors * Ichiro Matsumura View author publications You can also search for this author inPubMed Google Scholar * Andrei A Ivanov View author publications You can also search

for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Ichiro Matsumura. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE

Matsumura, I., Ivanov, A. How evolving enzymes can beat the heat and avoid defeat. _Nat Chem Biol_ 5, 538–539 (2009). https://doi.org/10.1038/nchembio0809-538 Download citation * Issue Date:

August 2009 * DOI: https://doi.org/10.1038/nchembio0809-538 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a

shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative