How the glycosyltransferase ogt catalyzes amide bond cleavage

ABSTRACT The essential human enzyme O-linked β-_N_-acetylglucosamine transferase (OGT), known for modulating the functions of nuclear and cytoplasmic proteins through serine and threonine

glycosylation, was unexpectedly implicated in the proteolytic maturation of the cell cycle regulator host cell factor-1 (HCF-1). Here we show that HCF-1 cleavage occurs via glycosylation of

a glutamate side chain followed by on-enzyme formation of an internal pyroglutamate, which undergoes spontaneous backbone hydrolysis. Access through your institution Buy or subscribe This is

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BASIS FOR LEWIS ANTIGEN SYNTHESIS BY THE Α1,3-FUCOSYLTRANSFERASE FUT9 Article 18 May 2023 STRUCTURAL BASIS FOR THE SYNTHESIS OF THE CORE 1 STRUCTURE BY C1GALT1 Article Open access 03 May

2022 GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN 1 (GLT8D1) IS A UDP-DEPENDENT GALACTOSYLTRANSFERASE Article Open access 07 December 2023 REFERENCES * Parker, J.B., Yin, H.,

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National Science and Engineering Research Council (NSERC) of Canada PGS-M and PGS-D3 fellowship recipient. Funding for this work was provided by a National Institutes of Health grant (R01

GM094263) to S.W. We thank C. Thompson (Bruker Daltonics, Billerica, Massachusetts, USA) for access to and assistance with a SolariX XR 7T q-Q-FT-ICR mass spectrometer. We thank D. Vocadlo

(Simon Fraser University, Vancouver, British Columbia, Canada) for providing UDP-5_S_GlcNAc. X-ray diffraction data were collected at the National Synchrotron Light Source at Brookhaven

National Laboratory (Beamline X25). AUTHOR INFORMATION Author notes * Michael B Lazarus Present address: Present address: Department of Pharmacology and Systems Therapeutics, Icahn School of

Medicine at Mount Sinai, New York, New York, USA., AUTHORS AND AFFILIATIONS * Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA John Janetzko 

& Michael B Lazarus * Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA John Janetzko, Michael B Lazarus & Suzanne Walker * Division of

Science, Small Molecule Mass Spectrometry, Harvard University, Cambridge, Massachusetts, USA Sunia A Trauger Authors * John Janetzko View author publications You can also search for this

author inPubMed Google Scholar * Sunia A Trauger View author publications You can also search for this author inPubMed Google Scholar * Michael B Lazarus View author publications You can

also search for this author inPubMed Google Scholar * Suzanne Walker View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS J.J. and S.W. designed

research and analyzed data; J.J. performed LC-MS/MS experiments with assistance from S.A.T.; M.B.L. performed X-ray crystallography experiments and solved the structure of hOGT4.5 (D554N);

J.J. performed all other experiments reported in the paper; J.J. and S.W. wrote the manuscript with input from all authors. CORRESPONDING AUTHOR Correspondence to Suzanne Walker. ETHICS

DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY TEXT AND FIGURES Supplementary Results, Supplementary Figures

1–22 and Supplementary Tables 1–11. (PDF 7286 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Janetzko, J., Trauger, S., Lazarus, M. _et al._ How the

glycosyltransferase OGT catalyzes amide bond cleavage. _Nat Chem Biol_ 12, 899–901 (2016). https://doi.org/10.1038/nchembio.2173 Download citation * Received: 20 December 2015 * Accepted:

06 July 2016 * Published: 12 September 2016 * Issue Date: November 2016 * DOI: https://doi.org/10.1038/nchembio.2173 SHARE THIS ARTICLE Anyone you share the following link with will be able

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