Automation of dna sequencing reactions and related techniques: a workstation for micromanipulation of liquids

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Automation of dna sequencing reactions and related techniques: a workstation for micromanipulation of liquids"


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ABSTRACT We describe the construction of a laboratory workstation for the micromanipulation of liquids. The performance of the workstation is demonstrated by its use in a DNA sequencing


protocol; although its operational units form the backbone of a robotic system with wide applications in molecular biology and biochemistry. Access through your institution Buy or subscribe


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PRINCIPLES OF COMPUTER-CONTROLLED LINEAR MOTION APPLIED TO AN OPEN-SOURCE AFFORDABLE LIQUID HANDLER FOR AUTOMATED MICROPIPETTING Article Open access 12 August 2020 SOFT ROBOTIC PATTERNING OF


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2024 REFERENCES * Sanger, F., Niklen, S., and Coulson, A.R. 1977. DNA sequencing with chain terminating inhibitors. _Proc. Natl. Acad. Sci. USA_ 74:5463–5467. Article  CAS  Google Scholar  *


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84:4767–4771. Article  CAS  Google Scholar  * Smith, L.M., Sanders, U.Z., Kaiser, R.J., Hughes, P., Dodd, C., Connell, C.R., Heiner, C., Kent, S.B.H., and Hood, L.E. 1986. Fluorescence


detection in automated DNA sequence analysis. _Nature_ 321:674–679. Article  CAS  Google Scholar  * Ansorge, W., Sproat, B.S., Stegemann, J., and Schwager, C. 1986. A nonradioactive


automated method for DNA sequence determination. _J. Biochem. Biophys. Meth._ 13:315–323. Article  CAS  Google Scholar  * Stoflet, E.S., Koeberl, D.D., Sarkar, G., and Sommer, S.S. 1988.


Genomic amplification with transcript sequencing. _Science_ 239:491–494. Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * Rainer Frank: Author to whom


correspondence should be sent. AUTHORS AND AFFILIATIONS * European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg, F.R. Germany Rainer Frank, Armin Bosserhoff, Christian


Boulin, Avi Epstein, Heinrich Gausepohl & Keith Ashman Authors * Rainer Frank View author publications You can also search for this author inPubMed Google Scholar * Armin Bosserhoff View


author publications You can also search for this author inPubMed Google Scholar * Christian Boulin View author publications You can also search for this author inPubMed Google Scholar * Avi


Epstein View author publications You can also search for this author inPubMed Google Scholar * Heinrich Gausepohl View author publications You can also search for this author inPubMed 


Google Scholar * Keith Ashman View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE


THIS ARTICLE Frank, R., Bosserhoff, A., Boulin, C. _et al._ Automation of DNA Sequencing Reactions and Related Techniques: A Workstation for Micromanipulation of Liquids. _Nat Biotechnol_ 6,


1211–1213 (1988). https://doi.org/10.1038/nbt1088-1211 Download citation * Received: 18 April 1988 * Accepted: 21 June 1988 * Issue Date: 01 October 1988 * DOI:


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