Automation of dna sequencing reactions and related techniques: a workstation for micromanipulation of liquids
Automation of dna sequencing reactions and related techniques: a workstation for micromanipulation of liquids"
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ABSTRACT We describe the construction of a laboratory workstation for the micromanipulation of liquids. The performance of the workstation is demonstrated by its use in a DNA sequencing
protocol; although its operational units form the backbone of a robotic system with wide applications in molecular biology and biochemistry. Access through your institution Buy or subscribe
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2024 REFERENCES * Sanger, F., Niklen, S., and Coulson, A.R. 1977. DNA sequencing with chain terminating inhibitors. _Proc. Natl. Acad. Sci. USA_ 74:5463–5467. Article CAS Google Scholar *
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_Nature_ 324:163–166. Article CAS Google Scholar * Dale, R.M.K., McClure, B.A., and Houchins, J.P. 1985. A rapid single-stranded cloning strategy for producing a sequential series of
overlapping clones for use in DNA sequencing: Application to sequencing the corn mitochondrial 18S rDNA. _Plasmid_ 13:31–40. Article CAS Google Scholar * Martin, W.J., Warmington, J.R.,
Galinski, B.R., Gallagher, M., Davies, R.W., Beck, M.S., and Oliver, S.G. 1985. Automation of DNA sequencing: A system to perform the sanger dideoxysequencing reactions. _Bio/Technology_
3:911–915. Article CAS Google Scholar * Martin, W.J., Galinski, B.R., and Beck, M.S. 1987. Automatic manipulation of microlitre volumes of liquid reagents. _J. Phys. E: Sci. Instrum._
20:22–26. Article CAS Google Scholar * Tabor, S. and Richardson, C.C. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. _Proc. Natl. Acad. Sci. USA_
84:4767–4771. Article CAS Google Scholar * Smith, L.M., Sanders, U.Z., Kaiser, R.J., Hughes, P., Dodd, C., Connell, C.R., Heiner, C., Kent, S.B.H., and Hood, L.E. 1986. Fluorescence
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automated method for DNA sequence determination. _J. Biochem. Biophys. Meth._ 13:315–323. Article CAS Google Scholar * Stoflet, E.S., Koeberl, D.D., Sarkar, G., and Sommer, S.S. 1988.
Genomic amplification with transcript sequencing. _Science_ 239:491–494. Article CAS Google Scholar Download references AUTHOR INFORMATION Author notes * Rainer Frank: Author to whom
correspondence should be sent. AUTHORS AND AFFILIATIONS * European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg, F.R. Germany Rainer Frank, Armin Bosserhoff, Christian
Boulin, Avi Epstein, Heinrich Gausepohl & Keith Ashman Authors * Rainer Frank View author publications You can also search for this author inPubMed Google Scholar * Armin Bosserhoff View
author publications You can also search for this author inPubMed Google Scholar * Christian Boulin View author publications You can also search for this author inPubMed Google Scholar * Avi
Epstein View author publications You can also search for this author inPubMed Google Scholar * Heinrich Gausepohl View author publications You can also search for this author inPubMed
Google Scholar * Keith Ashman View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE
THIS ARTICLE Frank, R., Bosserhoff, A., Boulin, C. _et al._ Automation of DNA Sequencing Reactions and Related Techniques: A Workstation for Micromanipulation of Liquids. _Nat Biotechnol_ 6,
1211–1213 (1988). https://doi.org/10.1038/nbt1088-1211 Download citation * Received: 18 April 1988 * Accepted: 21 June 1988 * Issue Date: 01 October 1988 * DOI:
https://doi.org/10.1038/nbt1088-1211 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not
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