Agrobacterium–mediated transformation of rice (oryza sativa l. )

ABSTRACT We have transformed embryo-derived cultures of two rice cultivars using several different _Agrobacterium_-mediated gene transfer systems. Mature embryos of cv. Nipponbare inoculated

with the wide host range (WHR) supervirulent strain A281(pTiBo542) formed tumorigenic callus tissue that grew on hormone-free medium. The transformed status of this tissue was confirmed by

DNA hybridization analysis that showed transferred DNA (T-DNA) present in the rice genome. Embryos of another variety, cv. Fujisaka 5, gave a hypersensitive response when inoculated with

strain A281 but exhibited extensive root proliferation following inoculation with the limited host range (LHR) strain A856. These roots grew on hormone-free medium and produced octopine.

Fujisaka 5 embryos subsequently inoculated with a disarmed WHR strain conferring kanamycin resistance and β-D-glucuronidase (GUS) activity produced callus that grew on selective levels of

kanamycin and this tissue fluoresced upon incubation with GUS substrate. GUS expression in the rice tissues was confirmed by Western blotting. We conclude that T-DNA has been transferred to,

integrated and then expressed in rice cells. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access

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Department of Botany, University of Maryland, College Park, MD, 20742 AUTHORS AND AFFILIATIONS * Department of Microbiology SC-42, University of Washington, Seattle, WA, 98195 D. M. Raineri 

& E. W. Nester * Department of Biochemistry SJ-70, University of Washington, Seattle, WA, 98195 M. P. Gordon Authors * D. M. Raineri View author publications You can also search for this

author inPubMed Google Scholar * P. Bottino View author publications You can also search for this author inPubMed Google Scholar * M. P. Gordon View author publications You can also search

for this author inPubMed Google Scholar * E. W. Nester View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Raineri, D., Bottino, P., Gordon, M. _et al._ _Agrobacterium_–Mediated Transformation of Rice (_Oryza sativa_ L.). _Nat Biotechnol_ 8, 33–38 (1990).

https://doi.org/10.1038/nbt0190-33 Download citation * Received: 26 June 1989 * Accepted: 30 October 1989 * Issue Date: 01 January 1990 * DOI: https://doi.org/10.1038/nbt0190-33 SHARE THIS

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