Seeing the full picture of metabolism

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Seeing the full picture of metabolism"


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Access through your institution Buy or subscribe Cells, tissues and organisms contain complex mixtures of chemicals, the products and by-products of metabolism. Although mass spectrometry


was routinely used to identify specific classes of metabolites starting in the mid-1950s (Milestone 7), it would take another decade before researchers began using the technique to examine


complex metabolite mixtures. In one early example, E.C. Horning and colleagues separated and characterized organic compounds from urine, mainly acids and steroids, using gas-liquid


chromatography and confirming chemical identity by mass spectrometry. In 1971, they extended their analysis to include drugs and drug metabolites, initiating the use of the method as a means


of establishing a 'metabolic profile' of an individual. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution


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Contact customer support REFERENCES ORIGINAL RESEARCH PAPERS * Dalgliesh, C.E., Horning, E.C., Horning, M.G., Knox, K.L. & Yarger, K. Gas-liquid-chromatographic procedure for separating


a wide range of metabolites occurring in urine or tissue extracts. _Biochem. J._ 101, 792–810 (1966) Article  CAS  Google Scholar  * Horning, E.C. & Horning, M.G. Metabolic profiles:


gas-phase methods for analysis of metabolites. _Clin. Chem._ 17, 802–809 (1971) CAS  PubMed  Google Scholar  * Han, X. & Gross, R.W. Electrospray ionization mass spectroscopic analysis


of human erythrocyte plasma membrane phospholipids. _Proc. Natl. Acad. Sci. USA_ 91, 10635–10639 (1994) Article  CAS  Google Scholar  * Brügger, B., Erben, G., Sandhoff, R., Wieland, F.T.


& Lehmann, W.D. Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. _Proc. Natl. Acad. Sci. USA_ 94,


2339–2344 (1997) Article  Google Scholar  * Fiehn, O. et al. Metabolite profiling for plant functional genomics. _Nat. Biotechnol._ 18, 1157–1161 (2000) Article  CAS  Google Scholar  *


Buchholz, A., Hurlebaus, J., Wandrey, C. & Takors, R. Metabolomics: quantification of intracellular metabolite dynamics. _Biomol. Eng._ 19, 5–15 (2002) Article  CAS  Google Scholar 


FURTHER READING * McFadden, W.H., Teranishi, R., Corse, J., Black, D.R. & Mon, T.R. Volatiles from strawberries. II. Combined mass spectrometry and gas chromatography on complex


mixtures. _J. Chromatogr._ 18, 10–19 (1965) Article  CAS  Google Scholar  * Allen, J. et al. _High-throughput classification of yeast mutants for functional genomics using metabolic


footprinting._ _Nat. Biotechnol._ 21, 692–696 (2003)10.1038/nbt823 Article  CAS  Google Scholar  * Fischer, E. & Sauer, U. Metabolic flux profiling of _Escherichia coli_ mutants in


central carbon metabolism using GC-MS. _Eur. J. Biochem._ 270, 880–891 (2003) Article  CAS  Google Scholar  * Smith, C.A., Want, E.J., O'Maille, G., Abagyan, R. & Siuzdak, G. XCMS:


processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification. _Anal. Chem._ 78, 779–787 (2006) Article  CAS  Google Scholar 


Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Associate Editor, Nature Communications, Kyle Legate Authors * Kyle Legate View author publications You can also search for


this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Legate, K. Seeing the full picture of metabolism. _Nat Methods_ 12


(Suppl 1), 14 (2015). https://doi.org/10.1038/nmeth.3529 Download citation * Published: 10 September 2015 * Issue Date: October 2015 * DOI: https://doi.org/10.1038/nmeth.3529 SHARE THIS


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