Interactions between bacterial carbon monoxide and hydrogen consumption and plant development on recent volcanic deposits

ABSTRACT Patterns of microbial colonization and interactions between microbial processes and vascular plants on volcanic deposits have received little attention. Previous reports have shown

that atmospheric CO and hydrogen contribute significantly to microbial metabolism on Kilauea volcano (Hawaii) deposits with varied ages and successional development. Relationships between CO

oxidation and plant communities were not clear, however, since deposit age and vegetation status covaried. To determine plant–microbe interactions in deposits of uniform ages, CO and

hydrogen dynamics have been assayed for unvegetated tephra on a 1959 deposit at Pu’u Puai (PP-bare), at the edge of tree ‘islands’ within the PP deposit (PP-edge) and within PP tree islands

(PP-canopy). Similar assays have been conducted for vegetated and unvegetated sites on a 1969 Mauna Ulu (MU) lava flow. Net _in situ_ atmospheric CO uptake was highest at PP-edge and PP-bare

sites (2.2±0.5 and 1.3±0.1 mg CO m−2 day−1, respectively), and least for PP-canopy (−3.2±0.9 mg CO m−2 day−1, net emission). Respiration rates, microbial biomass and maximum CO uptake

potential showed an opposing pattern. Comparisons of atmospheric CO uptake and CO2 production rates indicate that CO contributes significantly to microbial metabolism in PP-bare and

MU-unvegetated sites, but negligibly where vegetation is well developed. Nonetheless, maximum potential CO uptake rates indicate that CO oxidizer populations increase with increasing plant

biomass and consume CO actively. Some of these CO oxidizers may contribute to elevated nitrogen fixation rates (acetylene reduction) measured within tree islands, and thus, support plant

successional development. SIMILAR CONTENT BEING VIEWED BY OTHERS CHEMOSYNTHETIC AND PHOTOSYNTHETIC BACTERIA CONTRIBUTE DIFFERENTIALLY TO PRIMARY PRODUCTION ACROSS A STEEP DESERT ARIDITY

GRADIENT Article Open access 25 May 2021 GRASSLAND RESPONSES TO ELEVATED CO2 DETERMINED BY PLANT–MICROBE COMPETITION FOR PHOSPHORUS Article 10 July 2023 PHOTOSYNTHETIC MICROORGANISMS

EFFECTIVELY CONTRIBUTE TO BRYOPHYTE CO2 FIXATION IN BOREAL AND TROPICAL REGIONS Article Open access 28 July 2022 INTRODUCTION Volcanic landscapes typically consist of a mosaic of

successional states (Kitayama et al., 1995). Deposit age, degree of weathering, precipitation, temperature and availability of potential colonists interact with other variables to determine

patterns of ecosystem development at local-to-regional scales (Chadwick et al., 1999; Morisada et al., 2002). Analyses of these patterns have contributed to the formulation of ecological

theories and to a greater understanding of biogeochemical controls of ecosystem dynamics (Hooper and Vitousek, 1997; Vitousek and Farrington, 1997; Chadwick et al., 1999). For instance, the

roles of phosphorus, nitrogen and eolian nutrient inputs have been clearly documented through long-term observations of Hawaiian chronosequences (Crews et al., 1995; Chadwick et al., 1999;

Neff et al., 2000). Although they are among the first arrivals on volcanic deposits, relatively little is known about the temporal and spatial distributions of microbial colonists generally,

about specific microbial functional groups or about interactions among microorganisms and animal and plant communities. Vitousek and co-workers have addressed some of these issues through

their research on nitrogen fixation and nitrogen mineralization on Hawaiian volcanic deposits (Riley and Vitousek, 1995; Vitousek, 1999; Crews et al., 2001; Pearson and Vitousek, 2001).

Nüsslein and Tiedje (1998), Nanba et al. (2004) and Dunfield and King (2004) have documented various aspects of microbial community structure and function on recent Hawaiian volcanic

deposits (44–300 years old), and shown in particular that relatively young deposits harbored very distinct assemblages, especially where plants were present. Analyses of quinone profiles on

Mt Pinatubo mudflows also suggested possible associations of _Actinobacteria_ with developing plant communities (Ohta et al., 2003). Other groups have reported variable relationships among

plant community development, heterotrophic evenness, respiration, and C, N and P contents for successional transects on volcanic soils (Schippers _et al._, 2001). A survey of older soils of

Mt Etna has also shown that respiration varied independently of soil carbon content, and that plant litter decomposition varied as a function of soil development and plant litter source

(Hopkins et al., 2007). King (2003a) showed that microbial uptake of atmospheric CO and hydrogen accounted for a significant fraction of respiratory metabolism on volcanic deposits ranging

from about 21–210 years in age, but that these processes were relatively unimportant at a 300-year-old site supporting a mature forest. Results from Dunfield and King (2004, 2005) and

Gomez-Alvarez et al. (2007) also showed that microbial community structure changed markedly along this chronosequence, with both CO oxidizers and the microbial community as a whole dominated

by _Proteobacteria_ in the forested site, while other phyla, including many unidentified lineages, dominated the remaining sites. The observed patterns along the chronosequence may

partially reflect factors such as physical weathering, accumulated inputs of microbes from the atmosphere, and various stochastic and deterministic processes independent of plant succession.

They also undoubtedly reflect plant-dependent interactions. These may include species-specific interactions, such as those between actinorhizal or legume species (Ohtonen and Väre, 1998;

Smolander and Kitunen, 2002; de Neergaard and Gorissen, 2004) and their symbionts, or more general interactions, such as those resulting from inputs of organic matter (Kent and Triplett,

2002). Similar conclusions have been drawn for successional patterns associated with glacial retreat (Ohtonen et al., 1999; Sigler et al., 2002; Sigler and Zeyer, 2002). Results presented

here document interactions between plant succession and CO dynamics on volcanic deposits independent of substrate age. CO uptake rates at atmospheric and elevated concentrations and

comparisons of activities across gradients of plant development on volcanic deposits of a single age show that atmospheric CO is consumed most rapidly in deposits with little or no rooted

vegetation. In contrast, maximum potential uptake rates, an index of CO oxidizer biomass, are greatest where plant development and organic matter are greatest. These results suggest that

after initial colonization of newly formed substrates, CO oxidizer populations likely depend on organic inputs from plant communities for significant growth. Some of these CO oxidizers may

contribute to increases in acetylene reduction rates (King, 2003a), an index of nitrogen fixation, that were observed with increasing root biomass. MATERIALS AND METHODS SITE DESCRIPTION

Sites were established near Kilauea caldera on a 1959 tephra deposit (Pu’u Puai—PP) and a 1969 lava flow (Mauna Ulu—MU), aspects of which have been previously described (King, 2003a;

Dunfield and King, 2004; Nanba et al., 2004; Gomez-Alvarez et al., 2007). The PP site consists of islands of closed-canopy woody vegetation (mixed _Metrosideros polymorpha_ (Ohia) and

_Morella faya_ (also _Myrica faya_ or fire tree) interspersed with extensive unvegetated patches comprised of tephra (or volcanic cinders); the cinders were approximately 1 cm in diameter.

At PP, samples were collected within tree islands (PP-canopy), at the island edges 3–5 m from canopy centers (PP-edge) and 5 m distant from islands in unvegetated tephra (PP-bare). A

pronounced litter layer (approximately 5–10 cm thick) occurred within and at the edge of tree islands, while at bare sites, little or no litter was present. At MU, samples were collected

from cinder deposits that had accumulated on the surface of pahoehoe lava. Vegetation at MU is more patchy and sparse than at PP. Sample sites were colonized by a pioneering fern, _Sadleria

cyatheoides_ (MU-veg) or were unvegetated (MU-unveg). BULK PHYSICAL AND CHEMICAL ANALYSES Aluminum core tubes (7.2-cm inner diameter) were used to collect material to 5–10 cm depths. Water

contents were estimated by drying at 110 °C for >24 h. pH was determined using slurries of cinders and deionized water (1:2 mass ratio of samples and deionized water). Total organic

carbon and nitrogen were analyzed using a Perkin–Elmer model 2400 elemental analyzer for ground material from the upper two centimeters. Root biomass was determined by harvesting fine roots

(<2-mm diameter) from the upper 10 cm of material at each site. PHOSPHOLIPID PHOSPHATE ANALYSIS Phospholipid phosphate contents were analyzed as a surrogate for microbial biomass

(Brinch-Iversen and King, 1990). Triplicate 0.5- to 1-gram fresh weight (gfw) root-free samples from various depths were transferred to screw-cap glass tubes containing 5 ml of a 1:2 mixture

of dichloromethane /methanol. The tubes were sealed, transported to the laboratory on dry ice and subsequently stored at −20 °C prior to further processing. Dichloromethane (1.67 ml) and

deionized water (5 ml) were added to each tube prior to centrifugation (1000 _g_). Dichloromethane (1–2 ml) phases were transferred to glass ampoules and evaporated to dryness at 30 °C with

nitrogen. Phospholipid phosphate was liberated by persulfate digestion and measured colorimetrically (Brinch-Iversen and King, 1990). GAS FLUX ANALYSES Carbon monoxide and hydrogen fluxes

were measured _in situ_ using aluminum collars (about 67 cm2) fitted with a 1-l quartz chamber forming a static headspace (see King, 2003a). Gas exchange assays were initiated by sealing a

chamber to a collar, covering the chamber with aluminum foil, and removing 3-cm3 headspace samples using a needle and syringe at 3- to 4-min intervals for up to 15–20 min. Samples were

assayed in the field using a Trace Analytical RGD gas chromatograph (King, 1999). Starting ambient CO and hydrogen values were typically about 100 and 550 p.p.b., respectively. Additional

assays were conducted using triplicate intact cores (7.5-cm inner diameter × 20-cm length). Triplicate cores from PP and MU sites were returned to a field laboratory within a few hours after

collection and incubated at ambient temperature. After sealing the core tubes, core headspaces were subsampled for CO and hydrogen analysis as above. Analyses of CO2 production were

performed using headspace samples that were injected into an SRI flame ionization gas chromatograph equipped with a stainless steel column (3.2-mm outer diameter × 2 m) containing silica gel

and a methanizer to convert CO2 into methane. Samples for CO2 production were collected at intervals over a 48-h period during incubations at ambient temperature. Instrument responses to

CO2 and methane were determined using standards prepared from pure gases. Responses to CO and hydrogen were determined using standards at near-atmospheric levels or greater; they were

prepared by diluting pure gases in CO- and hydrogen-free air. Maximum potential CO and hydrogen uptake rates were estimated using fresh material from the upper 2-cm depth interval of each PP

and MU site. Triplicate 5-gfw samples were transferred to 110-cm3 jars that were sealed with neoprene stoppers and incubated in darkness at ambient temperature. After adding CO or hydrogen

to a final concentration of about 100 parts per million (p.p.m.; 1 p.p.m. is equivalent to about 0.1 kPa), jar headspaces were subsampled at intervals to determine uptake rates. Maximum

potential CO uptake rates were also estimated for selected depth intervals of PP sites. Similar assays were conducted with freshly collected, washed, soil-free fine (<2 mm) roots from _M.

polymorpha_, _M. faya_ and _S. cyatheoides_; triplicate samples of approximately 1 gfw of each root type were incubated in darkness in sealed jars with an ambient atmosphere (0.1 p.p.m. CO)

and with 100 p.p.m. CO as described by King and Crosby (2002). ACETYLENE REDUCTION RATES Triplicate intact cores (about 10 cm deep) from each of the PP and MU sites were collected using

aluminum core tubes (7.2-cm inner diameter). Acetylene was added to the core headspaces to a final concentration of about 5%. Sealed tubes were incubated at ambient temperature for about 72 

h. Headspace samples were obtained at intervals and transferred to evacuated 3-cm3 gas collection tubes containing 0.5 ml of ammoniacal silver nitrate to precipitate acetylene. Ethylene

(C2H4) concentrations in subsamples were analyzed by flame ionization gas chromatography using a Shimadzu GC-14A. Rates of C2H4 production provided a surrogate estimate of N2-fixation rates.

RESULTS BULK DEPOSIT PROPERTIES Root biomass varied dramatically among sites (Table 1) with a maximum in vegetated areas of 652±165 gram dry weight (gdw) per m2 for PP-canopy and minimum of

50±4 gdw m−2 for MU-veg. Unvegetated PP and MU sites contained negligible identifiable root mass. Total carbon and nitrogen contents paralleled root distributions with values up to 45.7 and

1.7%, respectively, in PP-canopy. C/N ratios were least in PP-bare (15.7), increasing by almost twofold in PP-canopy (27.2). In contrast, pH values decreased substantially from 6.9 in

PP-bare to 3.9 in PP-canopy. Water contents displayed the opposite trend, with PP-bare material holding very little water, while PP-edge and PP-canopy were relatively moist by comparison.

Trends between MU-unveg and MU-veg were comparable (Table 1). PHOSPHOLIPID PHOSPHATE CONTENT Phospholipid phosphate concentrations varied substantially among sites and with deposit depth

(Figure 1). PP-canopy surface material contained phospholipid concentrations more than an order of magnitude higher than surface material from any other site (_P_<0.001, analysis of

variance). Phospholipid phosphate concentrations were similar, however, for PP-edge and PP-bare sites at all depths. At all PP sites, phospholipid phosphate concentrations decreased by an

order of magnitude with increasing depth up to 10 cm; these changes were statistically significant (_P_<0.01, analysis of variance). At MU-veg, phospholipid phosphate concentrations were

an order of magnitude greater than at MU-unveg (_P_<0.01), and about 20-fold lower than at corresponding PP sites. GAS EXCHANGE Volcanic deposits at three of five sites consistently

consumed atmospheric CO: PP-bare, MU-veg and MU-unveg (Table 2). CO was emitted consistently from PP-canopy, but results were more variable for PP-edge, with both uptake and emission

observed (Table 2). Atmospheric CO uptake rates ranged between about 1.2 and 8.1 mg CO m−2 day−1, with no clear trends among sites. CO emission rates ranged from about 1.3 to 8.1 mg CO m−2 

day−1 (Table 2) for PP-canopy, with intermediate PP-edge values. _In situ_ CO exchange rates were similar to those for values obtained from _ex situ_ incubation of intact cores (Table 2).

Estimates for steady-state CO exchange concentrations (concentrations at which CO uptake equals CO production) ranged between 0 and 21 p.p.b. (parts per billion) for all but the PP-canopy

site (Table 2). For the latter, the steady-state concentration was 207±43 p.p.b., well above the typical ambient atmospheric concentrations (75–125 p.p.b.). CO uptake rate constants were

lowest in PP-canopy and MU-unveg (0.1 min−1) and significantly higher for the remaining sites (Table 2). For the MU sites, maximum potential CO uptake rates (0- to 2-cm depth interval) were

significantly greater (_P_<0.01) at MU-veg than MU-unveg. The MU values, however, were substantially less than values for comparable PP sites (Table 2). At PP-canopy and PP-edge sites,

maximum potential CO uptake rates decreased significantly (analysis of variance, _P_<0.01) with deposit depth (Figure 1a). For the 0- to 2-cm and 2- to 5-cm depth intervals, PP-canopy and

PP-edge rates were similar, but PP-canopy rates were significantly greater for the 5- to 10-cm interval. In contrast, maximum potential CO uptake rates were least for PP-bare, and did not

decline significantly with depth. With the exception of a single apparent outlier for the 0- to 2-cm depth interval of PP-edge, maximum potential CO uptake rates were strongly correlated

(_r_=0.99) with phospholipid phosphate concentrations (Figure 1b); a strong correlation (_r_=0.74) remains even if the apparent outlying point is included. All sites consumed atmospheric

hydrogen at rates generally greater than those for CO (Table 2). Trends in uptake versus vegetational development among sites were not statistically significant (_P_>0.5, analysis of

variance), although activity at PP-canopy was least with the highest threshold value (Table 2). Hydrogen was also consumed during _ex situ_ assays to determine maximum potential uptake

rates. Maximum potential hydrogen uptake rates were greater than the corresponding maximum potential CO uptake rates at all sites, and increased from MU-unveg to PP-canopy. A plot of paired

maximum potential hydrogen uptake and maximum potential CO uptake rates indicated that the capacity for hydrogen uptake rose exponentially to an asymptotic maximum as a function of increased

CO uptake capacity (Figure 2). Respiration rates, measured as CO2 production, increased with increasing vegetational development, and ranged from 3.2±0.6 to 103.7±22.8 and from 2.2±0.5 to

74.2±26.5 mmol m−2 day−1 for PP and MU sites, respectively (Table 2). Maximum potential CO uptake rates for the 0- to 2-cm interval of all sites were positively and linearly correlated with

respiration rates (_r_=0.71); maximum CO uptake potentials integrated over 0- to 10-cm depth for the PP sites were highly linearly correlated with respiration rates (_r_=0.99; Figure 3). The

relative contribution of atmospheric CO uptake to respiratory reducing equivalent flow for PP and MU was estimated by assuming a 1:1 stoichiometry for CO2 production and oxygen uptake, and

two and four reducing equivalents formed per mole of CO oxidized and CO2 produced, respectively. The percent of total respiratory reducing equivalent flow due to CO was calculated as: Since

both PP-canopy and PP-edge sites emitted CO during the time the respiration assay was conducted, the relative contribution of CO to respiratory reducing equivalent flow was zero; for

PP-bare, a value of 9.1% was obtained. For MU sites, the values ranged from negligible at MU-veg (0.2%) to 6.4% at MU-unveg (Table 2). ACETYLENE REDUCTION Acetylene reduction rates were

least in MU-unveg and PP-bare sites (0.4–0.8 μmol C2H2 reduced per m2 per day) and reached a maximum for PP-canopy samples (21.5 μmol C2H2 reduced per m2 per day; Table 2). Acetylene

reduction rates were positively and linearly correlated with root biomass across all sites (_r_=0.98), and positively and exponentially correlated with respiration rates (_r_=0.95). In

addition, acetylene reduction rates increased exponentially with increasing maximum CO uptake potential rates integrated over 10 cm depth for the PP sites (_r_=0.99); comparable depth

profiles were not available for the MU sites. ROOT CO PRODUCTION AND OXIDATION Live, fine roots from the fern, _S. cyatheoides_, and the trees, _M. polymorpha_ and _M. faya_, each produced

CO when incubated under ambient conditions. CO production rates for _S_. _cyatheoides_ roots obtained from two distinct sites (MU and a 1982 lava flow; 1.9–3.3 nmol gfw−1 h−1, respectively)

were comparable to rates for _M_. _faya_, 3.5±0.3 nmol gfw−1 h−1 and _M_. _polymorpha_, 1.2±0.1 nmol gfw−1 h−1 (Table 3). Maximum potential CO uptake rates were considerably higher than CO

production rates, and varied significantly among the three taxa. Essentially, identical uptake rates were observed for the two sets of _S_. _cyatheoides_ roots, 12.8±1.6 nmol CO oxidized per

gfw per h. Uptake rates for _M_. _polymorpha_ (9.6±0.6 nmol CO oxidized per gfw per h) were somewhat lower, while rates for _M_. _faya_ (24.4±1.6 nmol CO oxidized per gfw per h) were

somewhat higher. In general, there was a positive correlation between maximum potential root CO oxidation rates and root CO production rates (_r_=0.9; not shown). DISCUSSION Analyses of

receding glaciers and chronosequences of volcanic systems have revealed several aspects of microbial community responses to ecosystem succession, including temporal and spatial changes in

functional diversity, phylogenetic diversity (evenness and richness) and cell-specific activities. For example, Sigler et al. (2002) observed increases in cell number and total activity with

successional age, but maximal activity per cell and greater relative culturability occurred at intermediate stages, and phylotype richness decreased with age. Their results confirmed an

‘r-‘ to ‘K-strategy’ shift during succession, but indicated that successional changes were dynamic and occurring at both individual and community levels. Others have documented interactions

between microbes and vegetation, including changes in carbon utilization and community structure (for example, Ohtonen et al., 1999; Schipper et al., 2001). Previous analyses of Kilauea

volcano based on multiple sites that constituted a chronosequence from 21- to 300-year old showed that atmospheric CO and hydrogen utilization occurred early in successional development and

contributed significantly to respiratory metabolism (King, 2003a). Molecular ecological analyses also revealed significant differentiation among CO-oxidizing and lithotrophic communities

colonizing deposits of different ages and successional development (Dunfield and King, 2004, 2005; Nanba et al., 2004). In this study, we report interactions between successional

development, specific functional processes (CO and hydrogen oxidation and acetylene reduction) and related microbiological and biogeochemical variables for Kilauea volcano deposits with

uniform ages and climatic regimes. Data from both PP and MU sites show that atmospheric CO uptake contributes significantly (6–10%) to total respiratory activity (measured as CO2 production)

in unvegetated areas characterized by very low respiration rates and organic carbon concentrations (Tables 1 and 2). Atmospheric CO uptake rates at PP and MU unvegetated sites are similar

to previously reported estimates (King, 2003a); CO2 production rates fall within ranges reported for other organic carbon poor systems (Hopkins et al., 2007). These observations support a

role for CO as an energy source in carbon-limited systems, even when it is available at only very low concentrations (75–125 p.p.b.). In contrast, atmospheric CO contributes little or

nothing to respiratory activity at PP-canopy and -edge and MU-veg sites (Table 2). Even accounting for the possibility that root respiration might contribute a significant fraction of total

respiration at these sites (up to about 50%; Andrews et al., 1999; Wang et al., 2005), CO oxidation remains a minor fraction of bacterial activity. Instead, at these sites rhizodeposition

and leaf litter no doubt represent the primary sources of organic matter for respiration. These carbon sources support respiration rates for the PP and MU at levels similar to those reported

for other vegetated systems with moderate-to-high organic carbon concentrations (Campbell and Law, 2005; Vogel et al., 2005; Bernhardt et al., 2006). All of the sites assayed consume

atmospheric hydrogen, but there is little consistent variation among them (Table 2). Relative to carbon-based respiratory metabolism, atmospheric hydrogen uptake supports considerable

activity (Table 2). At sites MU-unveg and PP-bare, where organic carbon is limited, hydrogen uptake could contribute to biosynthesis, but CO2 fixation efficiencies are relatively low with

hydrogen lithotrophy (Bowien and Schlegel, 1981), and some hydrogen uptake appears due to exoenzymatic activity (King, 2003a). Although atmospheric CO contributes significantly to bacterial

metabolism at unvegetated sites, CO-oxidizing bacteria were active at all sites. This is evident from time courses of headspace CO concentrations during both _in situ_ and _ex situ_ analyses

(not shown). As in previous studies (King, 1999), a model for CO change based on a zero-order production term and a first-order uptake term explained patterns of CO uptake and emission. In

addition, cinders from all PP and MU sites consume exogenous CO added at elevated concentrations without a lag. Maximum potential CO uptake rates based on these assays correlate linearly

with estimates of microbial biomass (Figure 1b), and depth integrated maximum potential rates for PP correlate linearly with respiration rates (Figure 3). To the extent that maximum

potential CO uptake rates reflect CO oxidizer biomass, these relationships indicate that CO oxidizer biomass accounts for an approximately constant fraction of total biomass, and that

maximum potential activity likely responds to the same variables that determine respiratory activity. In particular, it appears that CO oxidizer biomass may become initially established on

barren deposits through inputs of atmospheric CO and exogenous organic matter (for example, in precipitation). Subsequently, CO oxidizer biomass expands with endogenous organic matter

production, especially that from rooted vegetation. This is consistent with both the preference of CO-oxidizing bacteria for organic substrates, and with their expression of CO oxidation

when substrate availability is low, as is typically the case in soil systems even when bulk organic concentrations are high (Morita, 1997). The distribution of maximum potential hydrogen

uptake rates among sites parallels that for maximum potential CO uptake rates (Table 2), suggesting some common controls for the two activities. This could be due in part to the fact that

some CO oxidizers also oxidize hydrogen (King and Weber, 2007). In addition, many hydrogen oxidizers, like virtually all CO oxidizers, are facultative lithotrophs that grow preferentially

with organic substrates (Bowien and Schlegel, 1981). However, when plotted as paired data, maximum potential hydrogen uptake rates increase exponentially to an asymptote as a function of

maximum potential CO uptake rates (Figure 3). This may reflect a shift from populations that oxidize both CO and hydrogen to populations that oxidize primarily CO as maximum potential CO

uptake rates increase. The fact that CO oxidizers remain active across a gradient of increasing ecosystem development, even though atmospheric CO decreases in significance as a substrate,

indicates that they may contribute to a variety of functions other than trace gas dynamics. Many CO-oxidizing isolates contain nitrogenase (King, 2003b). Some, such as CO-oxidizing rhizobia

(for example, _Bradyrhizobium japonicum_ USDA 6) contribute to symbiotic nitrogen fixation; others, such as CO-oxidizing _Burkholderia_ (for example, _B_. _xenovorans_ LB400), can contribute

to both asymbiotic and plant-associated nitrogen fixation (Chen et al., 2003; Choudhury and Kennedy, 2004). An exponential correlation between depth-integrated maximum potential CO uptake

and a surrogate measure of nitrogen fixation, acetylene reduction, for the PP sites indicates that controls of activity differ for the two processes, irrespective of the extent of

contributions by CO oxidizers. Differential controls for expression of the aerobic carbon monoxide dehydrogenase and nitrogenase systems likely include nitrogen and organic matter

availability, associative interactions with plant roots and responses to physical–chemical variables, for example, pH and water potential. In addition, populations of strictly heterotrophic

nitrogen fixers may proliferate to a much greater extent than nitrogen-fixing CO oxidizers in material at the canopy site, where acetylene reduction rates appear disproportionately high. Of

course, variations in acetylene reduction rates among sites may only partially reflect patterns in nitrogen fixation, since ratios of acetylene reduced to nitrogen fixed may also vary among

sites. Plant roots also affect the distribution and activity of CO oxidizers. Maximum potential CO uptake rates for MU and PP sites rise exponentially with root mass to an asymptote in

PP-edge and PP-canopy sites (Figure 4). This trend likely reflects an increase in organic matter availability that can support CO oxidizer biomass, but may also reflect an increase in CO

availability. In particular, roots of all three plant species examined in this study emit CO at rates comparable to rates previously reported for other plants (Table 3; King and Crosby,

2002). As a result, increases in root biomass across sites represent a gradient of increasing CO within the deposits, which may promote CO oxidizer biomass or activity. In addition, the

ability of roots of all three taxa to consume CO (Table 3) indicates that they may support CO oxidizer populations that can affect bulk and rhizosphere activities as well as exchanges with

the atmosphere (King and Crosby, 2002; King and Hungria, 2002). Interactions between plants and the diversity of CO-oxidizing populations are being assessed at present. In conclusion,

atmospheric CO supports significant metabolic activity on volcanic deposits lacking vascular plant growth. Relationships between maximum potential CO uptake, root mass, organic carbon and

estimates of microbial biomass indicate that subsequent to colonization on barren material, CO-oxidizing populations expand in concert with the microbial community as a whole, even though

the role of CO in metabolism overall diminishes with increasing plant development. CO oxidizers remain an active component of the microbial community throughout succession and may contribute

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507–514. Article  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, USA Gary M

King & Carolyn F Weber Authors * Gary M King View author publications You can also search for this author inPubMed Google Scholar * Carolyn F Weber View author publications You can also

search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Gary M King. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE King,

G., Weber, C. Interactions between bacterial carbon monoxide and hydrogen consumption and plant development on recent volcanic deposits. _ISME J_ 2, 195–203 (2008).

https://doi.org/10.1038/ismej.2007.101 Download citation * Received: 28 June 2007 * Revised: 02 October 2007 * Accepted: 02 October 2007 * Published: 29 November 2007 * Issue Date: February

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is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * biogeochemistry * carbon monoxide * volcanic