Clonal characterization of bone marrow derived stem cells and their application for bone regeneration

ABSTRACT Tissue engineering allows the design of functionally active cells within supportive bio‐scaffolds to promote the development of new tissues such as cartilage and bone for the

restoration of pathologically altered tissues. However, all bone tissue engineering applications are limited by a shortage of stem cells. The adult bone marrow stroma contains a subset of

nonhematopoietic cells referred to as bone marrow mesenchymal stem cells (BMSCs). BMSCs are of interest because they are easily isolated from a small aspirate of bone marrow and readily

generate single‐cell‐derived colonies. These cells have the capacity to undergo extensive replication in an undifferentiated state _ex vivo_. In addition, BMSCs have the potential to develop

either _in vitro_ or _in vivo_ into distinct mesenchymal tissues, including bone, cartilage, fat, tendon, muscle, and marrow stroma. Thus, BMSCs are an attractive cell source for tissue

engineering approaches. However, BMSCs are not homogeneous and the quantity of stem cells decreases in the bone marrow in aged population. A sequential loss of lineage differentiation

potential has been found in the mixed culture of bone marrow stromal cells due to a heterogenous population. Therefore, a number of studies have proposed that homogenous bone marrow stem

cells can be generated from clonal culture of bone marrow cells and that BMSC clones have the greatest potential for the application of bone regeneration _in vivo._ You have full access to

this article via your institution. Download PDF SIMILAR CONTENT BEING VIEWED BY OTHERS PURIFICATION AND FUNCTIONAL CHARACTERIZATION OF NOVEL HUMAN SKELETAL STEM CELL LINEAGES Article 14 June

2023 KINSHIP OF CONDITIONALLY IMMORTALIZED CELLS DERIVED FROM FETAL BONE TO HUMAN BONE-DERIVED MESENCHYMAL STROMA CELLS Article Open access 25 May 2021 OSTEOGENIC DIFFERENTIATION OF HUMAN

MESENCHYMAL STROMAL CELLS AND FIBROBLASTS DIFFERS DEPENDING ON TISSUE ORIGIN AND REPLICATIVE SENESCENCE Article Open access 07 June 2021 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND

AFFILIATIONS * Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia Yin Xiao, Shobha Mareddy & Ross Crawford Authors * Yin Xiao View

author publications You can also search for this author inPubMed Google Scholar * Shobha Mareddy View author publications You can also search for this author inPubMed Google Scholar * Ross

Crawford View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Yin Xiao. RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Xiao, Y., Mareddy, S. & Crawford, R. Clonal Characterization of Bone Marrow Derived Stem Cells and Their Application for Bone Regeneration. _Int J

Oral Sci_ 2, 127–135 (2010). https://doi.org/10.4248/IJOS10045 Download citation * Received: 29 May 2010 * Revised: 02 July 2010 * Accepted: 02 July 2010 * Published: 01 September 2010 *

Issue Date: 01 September 2010 * DOI: https://doi.org/10.4248/IJOS10045 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link

Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * bone morrow * stem cell

* mesenchymal * clonal culture * regenerative medicine * differentiation