Inhibition of hepatocyte growth factor-induced motility and in vitro invasion of human colon cancer cells by gamma-linolenic acid

ABSTRACT In this study we have determined the effects of the n-6 essential fatty acid gamma-linolenic acid (GLA) on the motility and invasive/metastatic nature of the human colon cancer cell

lines HT115, HT29 and HRT18. Cell motility was induced by hepatocyte growth factor/scatter factor (HGF/SF) and measured by both colony scattering and dissociation from carrier beads.

Invasiveness was measured in vitro by cellular invasion into extracellular matrix. At concentrations up to 100 microM (which had no effect on cell growth over the duration of the

experiments) both cell motility and invasion induced by HGF/SF were markedly reduced by GLA and its lithium salt. The attachment of these cells to the extracellular matrix components

(Matrigel and fibronectin) was also inhibited. There were also changes in the cell-surface E-cadherin, but not fibronectin receptor at similar concentrations. It is concluded that n-6

essential fatty acids have the ability to inhibit both motility and invasiveness of human colon cancer cells, perhaps by modifying cell-surface adhesion molecules. Access through your

institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 24 print

issues and online access $259.00 per year only $10.79 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to

local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT

BEING VIEWED BY OTHERS DECOUPLING CHEMICAL AND MECHANICAL SIGNALING IN COLORECTAL CANCER CELL MIGRATION Article Open access 10 February 2025 LINOLEIC AND OLEIC ACIDS ENHANCE CELL MIGRATION

BY ALTERING THE DYNAMICS OF MICROTUBULES AND THE REMODELING OF THE ACTIN CYTOSKELETON AT THE LEADING EDGE Article Open access 22 July 2021 ADDRESSING CANCER INVASION AND CELL MOTILITY WITH

QUANTITATIVE LIGHT MICROSCOPY Article Open access 31 January 2022 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Surgery, University of Wales College of Medicine, Cardiff, UK WG

Jiang Authors * WG Jiang View author publications You can also search for this author inPubMed Google Scholar * S Hiscox View author publications You can also search for this author

inPubMed Google Scholar * MB Hallett View author publications You can also search for this author inPubMed Google Scholar * C Scott View author publications You can also search for this

author inPubMed Google Scholar * DF Horrobin View author publications You can also search for this author inPubMed Google Scholar * MCA Puntis View author publications You can also search

for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Jiang, W., Hiscox, S., Hallett, M. _et al._ Inhibition of

hepatocyte growth factor-induced motility and in vitro invasion of human colon cancer cells by gamma-linolenic acid. _Br J Cancer_ 71, 744–752 (1995). https://doi.org/10.1038/bjc.1995.145

Download citation * Issue Date: 01 April 1995 * DOI: https://doi.org/10.1038/bjc.1995.145 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get

shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative