Inhibition of leukaemia cell proliferation by folic acid–polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into hl-60 cells

ABSTRACT The inhibitory effect of c-myb antisense oligodeoxynucleotides (ODNs) conjugated to folic acid (FA) on HL-60 cell proliferation was examined. Folic acid was covalently linked to a

polylysine chain and purified by gel chromatography. Sterile FA-polylysine was complexed with c-myb sense and antisense. Exposure of HL-60 cells to the FA-polylysine-c-myb antisense ODN

complex resulted in a down-regulation of c-myb expression and a greater inhibition of proliferation than that obtained using free ODNs. Moreover, FA-polylysine conjugate alone or complexed

to c-myb sense ODN was not toxic to cells. The antigenic properties and uptake of the vitamin were not affected by the polylysine chain. These data suggest that this strategy is potentially

useful for the selective delivery of anti-oncogene-targeted ODNs into cancer cells. Access through your institution Buy or subscribe This is a preview of subscription content, access via

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14 June 2024 THE CURRENT LANDSCAPE OF NUCLEIC ACID THERAPEUTICS Article 31 May 2021 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Laboratorio Chemioterapia Sperimentale, Istituto Tumori

Regina Elena, Roma, Italy G Citro Authors * G Citro View author publications You can also search for this author inPubMed Google Scholar * C Szczylik View author publications You can also

search for this author inPubMed Google Scholar * P Ginobbi View author publications You can also search for this author inPubMed Google Scholar * G Zupi View author publications You can also

search for this author inPubMed Google Scholar * B Calabretta View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Citro, G., Szczylik, C., Ginobbi, P. _et al._ Inhibition of leukaemia cell proliferation by folic acid–polylysine-mediated introduction of

c-_myb_ antisense oligodeoxynucleotides into HL-60 cells. _Br J Cancer_ 69, 463–467 (1994). https://doi.org/10.1038/bjc.1994.84 Download citation * Issue Date: 01 March 1994 * DOI:

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