The reed-sternberg cell/lymphocyte rosette. I. Properties of rosettes formed between hodgkin's cell lines and allogeneic lymphocytes

Nature

The reed-sternberg cell/lymphocyte rosette. I. Properties of rosettes formed between hodgkin's cell lines and allogeneic lymphocytes"


Play all audios:

Loading...

ABSTRACT The properties of rosettes formed between the Hodgkin's cell lines, L428 and L591, and allogeneic peripheral blood mononuclear cell populations have been investigated.


Immunocytochemical analysis showed that the majority of adherent cells were T-cells of both the CD4 and CD8 subsets. Only relatively few B-cells and monocytes were seen to adhere. However,


when peripheral blood mononuclear cell populations were fractionated, it was found that monocytes were as good as T-cells at forming rosettes with both L428 and L591, though B-cells were


shown to be poor at forming such associations. Treatment of both L428 and L591 with neuraminidase resulted in a significant reduction (P less than 0.01) in the mean number of adherent


lymphocytes and in the numbers of Hodgkin's tumour cells which formed rosettes. Smaller, less significant effects were observed for Cytochalasin B and trypsin. EDTA (10(-2) M) at pH 7.2


had no significant effect on rosetting for L428 or L591. Adherence of allogeneic lymphocytes to L428 or L591 was pH dependent but did not appear to correlate with cell surface charge.


Treatment of L428 cells with Fab fragments prepared from the IgG fraction of a hyperimmune rabbit anti-L428 antiserum, significantly (P less than 0.05) inhibited the adherence of allogeneic


lymphocytes, but only when used at high concentration. The binding requirements of the Hodgkin's cell lines with allogeneic peripheral blood lymphocytes, as described in this study,


appear to be quite different from those described for freshly isolated Hodgkin's tumour cells with autologous intratumoral lymphocytes. This suggests that the two phenomena may be


unrelated. There would appear to be an absolute requirement for cell surface sialic acid for allogeneic lymphocyte attachment to the HD cell lines. This might suggest that the


receptor-ligand system involved contains sialic acid as an integral part of the cell surface receptor structure involved in recognition of the appropriate ligand. Access through your


institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 24 print


issues and online access $259.00 per year only $10.79 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to


local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT


BEING VIEWED BY OTHERS G PROTEIN COUPLED RECEPTOR TRANSCRIPTS IN HUMAN IMMUNE CELLS AND PLATELETS Article Open access 27 September 2024 IDENTIFICATION, SORTING AND PROFILING OF FUNCTIONAL


KILLER CELLS VIA THE CAPTURE OF FLUORESCENT TARGET-CELL LYSATE Article 31 August 2023 SERUM COMPONENTS INFLUENCE ANTIBODY REACTIVITY TO GLYCAN AND DNA ANTIGENS Article Open access 22 August


2023 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * University Department of Pathology, Southampton General Hospital, UK DJ Flavell Authors * DJ Flavell View author publications You can also


search for this author inPubMed Google Scholar * DH Wright View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and


permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Flavell, D., Wright, D. The Reed-Sternberg cell/lymphocyte rosette. I. Properties of rosettes formed between Hodgkin's cell lines and


allogeneic lymphocytes. _Br J Cancer_ 59, 165–173 (1989). https://doi.org/10.1038/bjc.1989.35 Download citation * Issue Date: 01 February 1989 * DOI: https://doi.org/10.1038/bjc.1989.35


SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to


clipboard Provided by the Springer Nature SharedIt content-sharing initiative


Trending News

Ultralow-pressure-driven polarization switching in ferroelectric membranes

ABSTRACT Van der Waals integration of freestanding perovskite-oxide membranes with two-dimensional semiconductors has em...

Big increase in pediatric er visits for breathing problems from small san diego wildfire

2017 SAN DIEGO WILDFIRE INCREASED PEDIATRIC ER VISITS FOR BREATHING PROBLEMS A small wildfire in San Diego County in 201...

7 Questions on the Daily Impact of the Coronavirus

From how we work, socialize, and even pray, the coronavirus has upturned American life. The Pew Research Center’s Claudi...

Latest East Bay news and more | East Bay Times

Brentwood police chief to retire in SeptemberBrentwood Police Chief Tim Herbert will officially retire on Sept.5 after o...

Frequent allelic loss of the rb, d13s319 and d13s25 locus in myeloid malignancies with deletion/translocation at 13q14 of chromosome 13, but not in ly

ABSTRACT In order to identify a commonly deleted region of 13q14 on chromosome 13, we performed fluorescence _in situ_ h...

Latests News

The reed-sternberg cell/lymphocyte rosette. I. Properties of rosettes formed between hodgkin's cell lines and allogeneic lymphocytes

ABSTRACT The properties of rosettes formed between the Hodgkin's cell lines, L428 and L591, and allogeneic peripher...

410 Deleted by author — Medium

SitemapOpen in app Sign upSign inMedium LogoWrite Sign upSign inError410The author deleted this Medium story....

Fortnite buried treasure: week 4 season 8 challenge guide

Fortnite buried treasure is one of the week 4 season 8 challenges for Battle Royale. Search for buried treasure is one o...

Tips for Managing Debt During the Pandemic

2:17 Videos de AARP Tips for Managing Debt During the Pandemic Facebook Twitter LinkedIn Now is a great time to pay off ...

Assurance of salvation: anchored in god's promises and christ's work

Joel R. Beeke discusses the assurance of salvation from a Reformed theological perspective. He emphasizes that assurance...

Top