The effect of methotrexate on folate metabolism in the rat

ABSTRACT The metabolism of 2-[14C]-folic acid, 2-[14C]-5-methyltetrahydrofolate 5-[14C]-methyltetrahydrofolate, and a mixture of 2-[14C]-folic acid and 3',5',7,9-[3H]-folic acid

has been studied in rats that were dosed with methotrexate (MTX) 24 h before receiving the radioactive folate. Methotrexate increases urinary excretion of radioactivity in rats given

2-[14C]-folic acid, but there was no significant increase in urinary radioactivity in animals given 5-methyltetrahydrofolate. Animals dosed with MTX had less of the dose in the liver, and

excreted more of the dose via the faeces. These results are consistent with the known biochemical effects of methotrexate. Experiments with a mixture of 2-[14C]-folic acid and

3',5',7,9-[3H]-folic acid indicate that there is an increase in scission of the folate molecule following a dose of MTX. Access through your institution Buy or subscribe This is a

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SYNTHASE SUPPORTS TUMOUR TETRAHYDROFOLATE POOLS Article 18 November 2021 METHIONINE SYNTHASE IS ESSENTIAL FOR CANCER CELL PROLIFERATION IN PHYSIOLOGICAL FOLATE ENVIRONMENTS Article 18

November 2021 FOLATE METABOLISM: A RE-EMERGING THERAPEUTIC TARGET IN HAEMATOLOGICAL CANCERS Article Open access 11 March 2021 Authors * P A Barford View author publications You can also

search for this author inPubMed Google Scholar * J A Blair View author publications You can also search for this author inPubMed Google Scholar * M A Malghani View author publications You

can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Barford, P., Blair, J. & Malghani, M. The

effect of methotrexate on folate metabolism in the rat. _Br J Cancer_ 41, 816–820 (1980). https://doi.org/10.1038/bjc.1980.146 Download citation * Issue Date: 01 May 1980 * DOI:

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