Chemical transformation of chinese hamster cells. I. A comparison of some properties of transformed cells

ABSTRACT Fifty-one subclones from carcinogen-treated cells of 3 tissues (kidney, liver and prostate) of the male Chinese hamster have been studied to determine the relationships of 3

criteria of _in vitro_ transformation: morphological change, increased plating efficiency and growth in soft agar. There was no correlation between increased plating efficiency and the other

2 parameters. Morphological change was not always easily recognisable, particularly in cells derived from liver, and was not always a stable feature of any given subclone. This may be due

to the technique of isolation used (ring cloning) or may be due to chemically-treated cells requiring long periods of culturing before attaining a stable phenotype. When a stable

morphological appearance was achieved, there was good correlation between transformed morphology and colony formation in soft agar. The problems of scoring morphological change as an

assessment of malignant transformation, and the importance of spontaneous morphological changes are discussed. Access through your institution Buy or subscribe This is a preview of

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28 June 2021 Authors * D J Kirkland View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE

CITE THIS ARTICLE Kirkland, D. Chemical transformation of Chinese hamster cells. I. A comparison of some properties of transformed cells. _Br J Cancer_ 34, 134–144 (1976).

https://doi.org/10.1038/bjc.1976.135 Download citation * Issue Date: 01 August 1976 * DOI: https://doi.org/10.1038/bjc.1976.135 SHARE THIS ARTICLE Anyone you share the following link with

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