Cyclic amp regulates mrna expression of alpha-1d and alpha-2a adrenergic receptors in cultured human corpus cavernosum smooth muscle cells

ABSTRACT While the physiological effects of contractile (e.g. norepinephrine) and relaxatory (e.g. PGE1, forskolin) agents on corpus cavernosum smooth muscle tone have been characterized,

the regulation of alpha adrenergic receptor mRNA expression in erectile tissue remains to be investigated. The goal of this study was to investigate the modulation of alpha-1 and alpha-2

adrenergic receptor mRNA expression in cultured human corpus cavernosum smooth muscle cells in response to increased intracellular cAMP induced by prostaglandin E1 and forskolin. Human

corpus cavernosum smooth muscle cells were incubated for 24 h with or without PGE1 (5.7 μM), forskolin (10 μM) or an admixture of both. Total RNA was prepared from the cultures. Expression

of alpha-1d adrenergic receptor, alpha-2a adrenergic receptor and m2 muscarinic acetylcholine receptor was determined by RNase protection assays. Loading was normalized by RNase protection

of the housekeeping gene, cyclophilin A. The relative abundance of mRNAs was quantitated by scanning densitometry. Treatment of human corpus cavernosum smooth muscle cells with PGE1 or

forskolin resulted in decreased mRNA expression of alpha-1d and alpha-2a adrenergic receptors and m2 muscarinic acetylcholine receptor when compared to untreated cells. Combinations of PGE1

and forskolin produced a more pronounced decrease in mRNA than either agent alone. PGE1 and forskolin increased intracellular levels of cAMP in human corpus cavernosum smooth muscle cells

and combinations of both agents produced a more pronounced increase in cAMP synthesis. These results suggest that cAMP modulates the expression of alpha adrenergic receptors, one of the

principal contractile receptor systems in the corpora cavernosa. These observations further support the concept that erectile function is a balance between contractile and relaxatory

processes, which in turn regulate structure and function of the corpora cavernosa. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $259.00 per year only $21.58 per issue Learn more Buy this

article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in

* Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS ANO1 CHANNELS ARE EXPRESSED IN MOUSE URETHRAL SMOOTH MUSCLE BUT DO

NOT CONTRIBUTE TO AGONIST OR NEURALLY EVOKED CONTRACTIONS Article Open access 19 May 2025 AGEING INFLUENCES DETRUSOR CONTRACTIONS TO PROSTAGLANDIN, ANGIOTENSIN, HISTAMINE AND 5-HT

(SEROTONIN), INDEPENDENT TO THE RHO KINASE AND EXTRACELLULAR CALCIUM PATHWAYS Article Open access 23 October 2023 IN VITRO EFFECT OF RELAXIN IN THE RAT CORPUS CAVERNOSUM UNDER HYPERGLYCEMIC

AND NORMOGLYCEMIC CONDITIONS Article 12 December 2022 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Urology, Boston University School of Medicine, Boston, 02118, MA, USA A

Traish, NN Kim, Y-H Huang, I Goldstein & RB Moreland * Department of Biochemistry, Boston University School of Medicine, Boston, 02118, MA, USA A Traish Authors * A Traish View author

publications You can also search for this author inPubMed Google Scholar * NN Kim View author publications You can also search for this author inPubMed Google Scholar * Y-H Huang View author

publications You can also search for this author inPubMed Google Scholar * I Goldstein View author publications You can also search for this author inPubMed Google Scholar * RB Moreland

View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to A Traish. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT

THIS ARTICLE CITE THIS ARTICLE Traish, A., Kim, N., Huang, YH. _et al._ Cyclic AMP regulates mRNA expression of alpha-1d and alpha-2a adrenergic receptors in cultured human corpus cavernosum

smooth muscle cells. _Int J Impot Res_ 12 (Suppl 1), S41–S47 (2000). https://doi.org/10.1038/sj.ijir.3900501 Download citation * Received: 01 October 1999 * Accepted: 23 November 1999 *

Published: 25 May 2000 * Issue Date: 01 March 2000 * DOI: https://doi.org/10.1038/sj.ijir.3900501 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative

KEYWORDS * forskolin * cAMP * PGE1 * alpha adrenergic receptor * vascular smooth muscle * corpus cavernosum * erectile dysfunction