Neuronal and endothelial nitric oxide synthase isoforms: quantification of protein and mrna in the normal rat penis

ABSTRACT Nitric oxide synthase (NOS) is an important enzyme for erection. We evaluated the content of neuronal (nNOS) and endothelial (eNOS) isoforms and their mRNA in the penis and major

pelvic ganglion (MPG) of adult male rats by Western and Northern blot analysis. The cerebellum was evaluated as a control. nNOS protein and its mRNA were detected in abundance in the MPG,

cerebellum, pelvic urethra and within the crura of the penis. In contrast, the penile urethra, neurovascular bundle and the shaft of penis contained smaller amounts of this protein. eNOS

protein was most abundant in the penile and pelvic parts of the urethra, whereas a moderate level was found in the penile shaft, crura, neurovascular bundle, MPG and cerebellum. Similarly

eNOS mRNA was abundant in the penile and pelvic parts of the urethra, MPG and cerebellum. Penile shaft, crura and neurovascular bundle showed moderate amounts of eNOS mRNA. In conclusion,

nNOS and its mRNA are most abundant in the MPG and crura of penis whereas eNOS is most abundant in the urethra and to a lesser extent present in the penis. Importantly eNOS protein and mRNA

were demonstrated in the MPG, where eNOS function has to be studied. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution

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about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS FIBER COUNTS AND ARCHITECTURE OF THE HUMAN DORSAL PENILE NERVE Article

Open access 31 May 2023 IMMUNOHISTOLOGICAL STUDY OF THE DENSITY AND DISTRIBUTION OF HUMAN PENILE NEURAL TISSUE: GRADIENT HYPOTHESIS Article 02 May 2022 CHRONIC PROSTATITIS/CHRONIC PELVIC

PAIN SYNDROME IMPAIRS ERECTILE FUNCTION BY INDUCING APOPTOSIS IN A RAT MODEL OF EXPERIMENTAL AUTOIMMUNE PROSTATITIS Article 21 August 2024 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *

Department of Urology, Suez Canal University, Ismailia, Egypt RM Seyam * Division of Urology, Department of Surgery, McGill University, Montreal, Quebec, Canada HT Huynh & GB Brock

Authors * RM Seyam View author publications You can also search for this author inPubMed Google Scholar * HT Huynh View author publications You can also search for this author inPubMed 

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ARTICLE Seyam, R., Huynh, H. & Brock, G. Neuronal and endothelial nitric oxide synthase isoforms: Quantification of protein and mRNA in the normal rat penis. _Int J Impot Res_ 11,

301–308 (1999). https://doi.org/10.1038/sj.ijir.3900451 Download citation * Received: 28 April 1998 * Revised: 30 October 1998 * Accepted: 24 May 1999 * Published: 12 January 2000 * Issue

Date: 01 December 1999 * DOI: https://doi.org/10.1038/sj.ijir.3900451 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry,

a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * nitric oxide synthase * mRNA *

penis * major pelvic ganglion * urethra