A map of fear | Nature Reviews Genetics

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A map of fear | Nature Reviews Genetics"


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Mott et al. report a solution to the twofold problem of stock homogeneity and the need for a finer QTL mapping resolution. Their improved technique is illustrated by mapping five QTL for


emotionality in mice.


The classical way of mapping QTL involves crossing two inbred lines that differ in the mean phenotype of a given trait. Repeated intercrosses or backcrosses onto one parental strain are then


conducted to single out the regions with the QTL from the rest of the genome. To increase the resolution in QTL mapping, Mott et al. abandoned simple crosses between inbred lines in their


mapping studies in favour of a genetically heterogeneous stock, which was established 30 years ago from eight inbred founder strains. The highly recombinant nature of the heterogeneous stock


genome (which is a fine genetic mosaic of its founders) gives a 30-fold increase in the resolution for QTL mapping, allowing mapping of QTL to under 0.5 cM with fewer than 2,000 animals.


Because the heterogeneous stock was derived from eight different lines, it is also genetically diverse.


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