Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells

ABSTRACT Drought is a major threat to agricultural production. Plants synthesize the hormone abscisic acid (ABA) in response to drought, triggering a signalling cascade in guard cells that

results in stomatal closure, thus reducing water loss1. ABA triggers an increase in cytosolic calcium in guard cells ([Ca2+]cyt)2,3,4,5,6 that has been proposed to include Ca2+ influx across

the plasma membrane3,5,7,8,9. However, direct recordings of Ca2+ currents have been limited3 and the upstream activation mechanisms of plasma membrane Ca2+ channels remain unknown. Here we

report activation of Ca2+-permeable channels in the plasma membrane of _Arabidopsis_ guard cells by hydrogen peroxide. The H2O2-activated Ca2+ channels mediate both influx of Ca2+ in

protoplasts and increases in [Ca2+]cyt in intact guard cells. ABA induces the production of H2O2 in guard cells. If H2O2 production is blocked, ABA-induced closure of stomata is inhibited.

Moreover, activation of Ca2+ channels by H2O2 and ABA- and H2O2-induced stomatal closing are disrupted in the recessive ABA-insensitive mutant _gca2_. These data indicate that ABA-induced

H2O2 production and the H2O2-activated Ca2+ channels are important mechanisms for ABA-induced stomatal closing. Access through your institution Buy or subscribe This is a preview of

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STOMATAL RESPONSE IN GUARD CELLS THROUGH PH REGULATION Article Open access 05 February 2024 HYDROGEN PEROXIDE IS REQUIRED FOR LIGHT-INDUCED STOMATAL OPENING ACROSS DIFFERENT PLANT SPECIES

Article Open access 14 June 2024 GUARD CELL ENDOMEMBRANE CA2+-ATPASES UNDERPIN A ‘CARBON MEMORY’ OF PHOTOSYNTHETIC ASSIMILATION THAT IMPACTS ON WATER-USE EFFICIENCY Article 29 July 2021

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Scholar  Download references ACKNOWLEDGEMENTS We thank J. Satimanont and C. Kwak for assistance; R. Tsien and M. Yanofsky for use of imaging systems and fluorescence microscopes; and J.

Kwak, D. Lee, R. Vaidyanathan, M. Ghassemian, N. Leonhardt and P. Buschmann for comments on the manuscript. This work was supported by Department of Energy, National Institutes of Health and

National Science Foundation grants to J.I.S. and by a European Biotech Program grant and Fonds der Chemischen Industrie to E.G. Y.M was supported by a fellowship for Scientific Research

from the Ministry of Education, Science, Sports and Culture of Japan, S.T and G.A by Human Frontier Science Program fellowships, and B.K. by a Feodor-Lynen fellowship from the Alexander von

Humboldt Foundation. AUTHOR INFORMATION Author notes * Zhen-Ming Pei, Yoshiyuki Murata, Gregor Benning and Erwin Grill: These authors contributed equally to this work AUTHORS AND

AFFILIATIONS * Division of Biology, Cell and Developmental Biology, and Center for Molecular Genetics, University of California at San Diego, La Jolla, 92093-0116, California, USA Zhen-Ming

Pei, Yoshiyuki Murata, Sébastien Thomine, Birgit Klüsener, Gethyn J. Allen & Julian I. Schroeder * Technische Universität München, Lehrstuhl für Botanik, Freising–Weihenstephan ,

D-85350, Germany Gregor Benning & Erwin Grill Authors * Zhen-Ming Pei View author publications You can also search for this author inPubMed Google Scholar * Yoshiyuki Murata View author

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Thomine View author publications You can also search for this author inPubMed Google Scholar * Birgit Klüsener View author publications You can also search for this author inPubMed Google

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inPubMed Google Scholar * Julian I. Schroeder View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Julian I. Schroeder.

RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Pei, ZM., Murata, Y., Benning, G. _et al._ Calcium channels activated by hydrogen peroxide mediate

abscisic acid signalling in guard cells. _Nature_ 406, 731–734 (2000). https://doi.org/10.1038/35021067 Download citation * Received: 14 February 2000 * Accepted: 18 May 2000 * Published: 17

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