Gene therapy progress and prospects. Downregulating gene expression: the impact of rna interference

ABSTRACT The control and maintenance of gene expression is critical for cell development and differentiation. Over the last 2 years, our understanding of the role of RNA as a regulator of

gene expression has significantly increased. Small RNA molecules are key elements of a machinery that trigger chromosomal modifications, post-transcriptional gene silencing and protein

translational blockade depending on the source, the RNA and the nature of the interaction with the target nucleic acid. Currently, the best characterized of this group of RNA-mediated gene

regulation pathways is the post-transcriptional gene silencing mechanism known as RNA interference. RNAi is triggered by double-stranded RNA (dsRNA), which induces the formation of a

ribonucleoprotein complex that mediates sequence-specific cleavage of the transcript cognate with the input dsRNA. RNAi has been adapted as a functional genomics tool and it has potential as

a therapeutic approach. This review will summarize our current understanding of the RNAi mechanism and the various applications of RNAi-based technologies. Access through your institution

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OTHERS TARGETING NON-CODING RNAS TO OVERCOME CANCER THERAPY RESISTANCE Article Open access 13 April 2022 AN OLD FRIEND WITH A NEW FACE: TRNA-DERIVED SMALL RNAS WITH BIG REGULATORY POTENTIAL

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and Technology Partnerships, Office of the Director, Center for Cancer Research, NCI, Bethesda, MD, USA N J Caplen Authors * N J Caplen View author publications You can also search for this

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expression: the impact of RNA interference. _Gene Ther_ 11, 1241–1248 (2004). https://doi.org/10.1038/sj.gt.3302324 Download citation * Published: 04 August 2004 * Issue Date: 01 August 2004

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currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * RNA interference (RNAi) * gene silencing * short

(small) interfering RNAs (siRNAs)